Transcriptome Profiling of the Salt-Stress Response in the Halophytic Green Alga Dunaliella salina

Plant Molecular Biology Reporter - Due to the hypersaline environment cell of Dunaliella salina can change its morphology, growth, and pigment for adapting to the stress. Despite the fact D. salina...     

光刺激移植视网膜诱导大鼠上丘FOS的表达

目的 用光反复刺激移植视网膜,观察脑内上丘c-fos的表达情况,检测移植视网膜能否对光刺激作出反应。方法 设实验组与对照组,用免疫组化方法显示脑内上丘c—fos的表达。结果 实验组移植视网膜与非移植视网膜大鼠在光反复刺激后1小时,脑内上丘出现明显的c-fos免疫反应阳性神经元,停止光刺激后2～3小时阳性神经元数量达到高峰。结论 光刺激移植视网膜能诱导脑内上丘c—fos的表达,提示移植视网膜具有对光刺激作出反应的能力。     

EAD: elasticity aware deduplication manager for datacenters with multi-tier storage systems

The popularity of Big Data applications places pressures on storage systems to efficiently scale to meet the demand. At the same time, new developments like solid-state drives have changed to traditional storage hierarchy. Cloud storage systems are transitioning towards a hybrid architecture consisting of large amounts of memory, solid-state disks (SSDs), and traditional magnetic hard disks (HD). This paper presents elasticity aware deduplication (EAD), a data deduplication framework designed for multi-tier cloud storage architectures consisting of SSD and HD. EAD dynamically adjusts the deduplication parameters at runtime in order to improve performance. Experimental results indicate that EAD is able to detect more than 98% of all duplicate data, but it only consumes less than 5% of expected memory space. Additionally, EAD saves approximately 74% of overall IO access cost compared to the traditional design.     

Th1/Th2类细胞因子的变化对急性免疫性肝损伤的影响研究

目的探讨Th1/Th2类细胞因子的变化对ConA诱导的急性免疫性肝损伤的机制,以及脾脏对急性免疫性肝损伤的影响作用。方法将Balb/c小鼠随机分为两组：正常对照组,肝损伤组。正常对照组尾静脉注射等量生理盐水,肝损伤组尾静脉注射12.5mg/Kg ConA一次。各组分别于ConA注射后8h,24h,72h取材,进行下列研究：①HE染色观察各组小鼠肝脏病理学改变。②经眼球取血,收集血清测ALT和AST。③收集各组小鼠血清及新鲜肝、脾组织（各100mg）,获取肝、脾组织裂解液。用多参数细胞因子检测技术即FlowCytomix技术,通过流氏细胞仪对荧光素PE信号强度的检测,实现对各组小鼠血清、肝组织、脾组织内多种Th1/Th2类因子的定性定量分析。结果①HE染色：正常对照组肝组织结构正常。肝损伤组8h时表现为急性肝损伤表现,24h时可见大片坏死灶,72h时肝损伤缓解。②血清ALT和AST检测：正常对照组3个时间点内无明显升高,肝损伤组3个时间段内ALT和AST均高于正常对照组,有显著性差异。③Th1/Th2细胞因子检测结果：肝脏：肝损伤组8h时Th1和Th2类细胞因子均明显升高,与正常对照组比较有显著性差异,24h后开始下降,降至正常水平或正常水平以下,呈明显下降趋势。血清：肝损伤组Th1,Th2类细胞因子8h均升高,24h后逐步降低。脾脏：肝损伤组Th1,Th2类细胞因子8h时均升高,与正常对照组比较,有显著性差异,24h时明显降低。结论①ConA诱导的急性免疫性肝损伤主要是由Th1类细胞、巨噬细胞和Th2类细胞分泌的炎性因子所造成。②脾脏通过Th1/Th2类细胞因子的分泌对急性免疫性肝损伤起到免疫调控作用。     

Characterization and comparisons of microbiota in different intestinal segments between adult Chinese Shanxi Black Pigs and Large White Pigs

The carcass weight of Chinese Shanxi Black Pigs is relatively lower in comparison with that of the counterparts, i.e., Large White Pigs, although the former are resistant to harsh conditions. Since gut microflora has been recognized to play a key role in pork production, it is of interest to explore the microbial communities in different intestinal segments of pigs and its potential relatedness with host features. In this study, 16S rRNA gene amplicon sequencing, accompanied by the inter- and intra-group comparisons, was implemented to investigate the structure composition and potential functions of microbial communities of four distinct intestinal segments [duodenum (D), jejunum (J), ileum (I), and cecum (C)] between adult Chinese Shanxi Black Pigs and Large White Pigs. Comparative survey revealed that the dominant phylum in both breeds was Firmicutes, followed by Proteobacteria, and Bacteroidetes. At the genus level, Lactobacillus was predominant in all samples, and Prevotella was specifically prevalent in the cecum. Further inspection showed the differences of dominant species in the same segments between these two groups. Notably, unique taxa in C and D segments were more than that in I and J segments. Additionally, each segment was characterized by specifically enriched genera, and distinctive pathways were predicted in certain intestinal segments. In short, the findings presented a coherent picture of structure composition and predicted functionalities of gut microbiota in diverse intestinal segments of adult Chinese Shanxi Black Pigs and Large White Pigs, and extend the understanding of potential link between intestinal microbiota and their hosts.     

Gene expression profile analysis of human intervertebral disc degeneration

In this study, we used microarray analysis to investigate the biogenesis and progression of intervertebral disc degeneration. The gene expression profiles of 37 disc tissue samples obtained from patients with herniated discs and degenerative disc disease collected by the National Cancer Institute Cooperative Tissue Network were analyzed. Differentially expressed genes between more and less degenerated discs were identified by significant analysis of microarray. A total of 555 genes were significantly overexpressed in more degenerated discs with a false discovery rate of < 3%. Functional annotation showed that these genes were significantly associated with membrane-bound vesicles, calcium ion binding and extracellular matrix. Protein-protein interaction analysis showed that these genes, including previously reported genes such as fibronectin, COL2A1 and β-catenin, may play key roles in disc degeneration. Unsupervised clustering indicated that the widely used morphology-based Thompson grading system was only marginally associated with the molecular classification of intervertebral disc degeneration. These findings indicate that detailed, systematic gene analysis may be a useful way of studying the biology of intervertebral disc degeneration.     

CTx促进远端视神经切断后视网膜节细胞轴突再生与c-Jun表达的关系

目的探讨霍乱毒素(CTx)促进成年金黄地鼠视神经远端切断后视网膜节细胞(RGCs)轴突再生与c-Jun的表达关系。方法远端切断视神经并对接一段自体坐骨神经,玻璃体内注射CTx及/或植入小段坐骨神经分支(SN)。动物随机分为AG CTx组;AG SN组;AG SN CTx组,各组动物分别存活4W,用荧光金(FG)逆行标记和c-Jun免疫荧光组织化学双标法观察轴突再生的RGCs内c-Jun表达情况。结果再生RGCs内有c-Jun蛋白表达,玻璃体内给予CTx或植入SN组RGCs表达c-Jun的再生RGCs分别为35·8±9·57和32·2±7·25个,约占其再生总数的94%及90%,两组相比无显著性差异(P>0·05);CTx与SN联用组c-Jun阳性再生的RGCs为150·2±43·92个,占再生总数的97%,与前两组相比,均有显著性差异(P<0·05)。结论视神经远端切断后约90%以上的再生RGCs有c-Jun表达,提示c-Jun表达与视神经远端受损后节细胞轴突再生密切相关,CTx及外周神经对RGCs轴突再生及c-Jun表达有协同促进作用。     

视神经远端切断后再生视网膜节细胞Bcl-2的表达

目的　成年金黄地鼠视神经远端切断后再生视网膜节细胞 (RGCs)Bcl 2的表达与再生的关系。方法　远端切断视神经并对接一段自体坐骨神经 ,玻璃体内注射CTx及 /或植入小段坐骨神经分支 (SN)。动物随机分为AG +CTx组 ;AG +SN组 ;AG +SN +CTx组 ,各组动物分别存活 4W ,用粒蓝 (GB)逆行标记和Bcl 2免疫荧光组织化学双标法观察再生的RGCs和Bcl 2表达。结果　再生RGCs胞质内有Bcl 2蛋白表达 ,玻璃体内给予CTx或植入SN组表达Bcl 2的再生RGCs分别为 32 2± 4 71和 2 9 4± 3 75个 ,约占其再生总数的 82 5 0 %及 80 96 % ,两组相比无显著性差异 (P >0 0 5 ) ;CTx与SN联用组Bcl 2阳性再生的RGCs为 15 1 8± 35 6 9个 ,占再生总数的 91 2 2 % ,与前两组相比 ,均有显著性差异 (P <0 0 5 )。结论　视神经远端切断后约 80 %的再生RGCs有Bcl 2表达 ,提示Bcl 2表达可能与节细胞再生有密切关系。     

C-fos基因在大鼠缺血视网膜内的表达

实验用FOS免疫组化方法（ABC法）研究了缺血诱导的大鼠视网膜内c-fos原癌基因的表达情况。实验动物用升高眼压的方法做成视网膜缺血模型,依缺血后存活时间不同分15′、30′、1h、2h、4h、6h、12h七个组,每只大鼠右眼为缺血眼,左眼做自身对照眼,另设正常对照组。动物腹腔麻醉,4％多聚甲醛灌注固定,取双眼冰冻切片,片厚15μm。实验结果显示缺血后15′组大鼠视网膜内核层最先出现少量卵圆型浅棕色的FOS阳性神经元胞核,30分钟至1h FOS表达逐渐增强,节细胞层也出现FOS阳性胞核。缺血后2h FOS表达达最高峰,缺血后4h FOS阳性胞核逐渐减少,12h达正常组水平、自身对照眼及正常对照眼网膜节细胞层偶见FOS阳性胞核。