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1.
Díez-Baños P Pedreira J Sánchez-Andrade R Francisco I Suárez JL Díaz P Panadero R Arias M Painceira A Paz-Silva A Morrondo P 《The Journal of parasitology》2008,94(4):925-928
A coprological survey to analyze the presence of flock resistance to benzimidazoles (BZ) and macrocyclic lactones (ML) was performed in sheep under field conditions. Fecal samples were collected from 2,625 sheep in 72 commercial farms from Galicia (NW Spain). The in vitro (FECRT, fecal egg count reduction test) and in vivo (EHA, egg hatch assay, and LFIA, larval feeding inhibition assay) tests were used to assess the efficacy of these anthelmintics. Coprocultures were also developed to obtain knowledge on the main genera of trichostrongylid nematoda prior to, and after, the administration of the anthelmintics. By using the FECRT, BZ resistance was observed in 13 (18%) flocks, whereas ML resistance was only detected in 2 (3%) farms. The number of resistant flocks to BZ was 21 (29%) by using the EHA and 7 (10%) by means of the LFIA. None of the flocks used in this study showed simultaneous resistance to both employed anthelmintics. The results from the in vitro and in vivo tests revealed that 92% of the flocks FECRT resistant to BZ were also resistant with the EHA. The LFIA confirmed all the farms resistant to ML by using the in vivo test. After the administration of BZ, nematode larvae belonging to Teladorsagia circumcincta (32.2%), Trichostrongylus spp. (29%), Nematodirus spp. (6.5%), and Chabertia ovis (3.2%) were identified. In the flocks receiving ML, only T. circumcincta was identified (57%). We recommend the use of in vitro tests because they are more efficient. As the use of macrocyclic lactones is increasing in this region, further investigation is needed for detecting resistance to the anthelmintic family compounds by the LFIA. 相似文献
2.
Luís Vázquez Rosario Panadero Vicente Dacal Francisco Javier Pato Ceferino López Pablo Díaz María Sol Arias Gonzalo Fernández Pablo Díez-Baños Patrocinio Morrondo 《Experimental & applied acarology》2011,53(4):399-409
During the 2007 and 2008 hunting seasons (April–October) the skin of 367 roe deer (Capreolus capreolus L.), hunted in different preserves from Galicia (Northwestern Spain), were examined for ticks (Acari: Ixodidae). The overall
prevalence of infestation by ticks was 83.1%. The predominant species was Ixodes ricinus (83.1%), whereas a single Dermacentor marginatus specimen appeared in one roe deer. All developmental stages of I. ricinus were found parasitizing roe deer, the adults being the most frequent (82.2%), followed by nymphs (45.6%) and larvae (27.2%).
The mean intensity of infestation by I. ricinus was 43.2 ± 49.85; most of them were adults (30.7 ± 31.64) and in a lesser extend nymphs (16.9 ± 24.74) and larvae (10.7 ± 29.90).
Ixodes ricinus was present all over the study with percentages that oscillated between 100% in spring and 57.4% in autumn. CHAID algorithm
showed the sex of roe deer as the most influential factor in tick prevalence, followed by the climatic area. The different
developmental stages of I. ricinus were more frequent in males than in females, and the prevalence of adults and larvae were higher in roe deer from coastal
areas than in those from mountainous and central areas, whereas nymphs were more frequent in mountainous areas. Host age and
density were not determinants for tick infestation. Our results confirm that roe deer are important hosts for I. ricinus in northwestern Spain, serving as a vehicle for the geographic distribution of these ticks. 相似文献
3.
Fernando Amor Patrocinio Ortega Xim Cerdá Raphaël R. Boulay 《Ethology : formerly Zeitschrift fur Tierpsychologie》2011,117(11):1031-1039
Nycthemeral rhythm is an important biological trait that allows animals to escape predation and competition and, conversely, to coincide with mutualists. Although laboratory studies have shown that the rhythm depends on both endogenous factors and cyclic environmental cues, the latter is often poorly understood, particularly in the wild. Because insects are mostly ectothermal organisms, their activity rhythm is often thought to depend directly on ground temperature. In Mediterranean habitats, Cataglyphis ants are well known for their unusual thermoresistance, allowing them to forage in summer at the central hours of the day when the ground reaches temperatures that are lethal to their competitors. However, we show that the rhythm of Cataglyphis floricola in south‐western Spain is governed by light cues rather than by temperature. First, variations in ant traffic at the nest entrance were better explained by solar elevation angle than by ground temperature on both seasonal and daily scales. Second, if ants waited for the ground to reach a threshold temperature to start their activity, we would expect similar temperatures regardless of the opening hour. However, we found a significant increase in ground temperature as opening hour got later in the day. Third, by using a simple experimental set‐up that increased the apparent solar elevation over the nest entrance, we provoked a delay of nest closure time. We discuss the relevance of these results with respect to the life history of Cataglyphis species and their possible consequences in relation to global warming. 相似文献
4.
P. Díaz S. Remesar J. M. Venzal M. E. Vzquez‐Lpez G. Fernndez C. Lpez P. Díez‐Baos P. Morrondo R. Panadero 《Medical and veterinary entomology》2019,33(3):427-430
Ixodes ricinus, comprising the predominant tick species in Europe, can transmit important human pathogens, including Borreliella spp., the causal agent of Lyme borreliosis. One hundred and seventy five roe deer hunted in two areas (plateau and mountain) of Galicia (northwest Spain) were examined for the presence of ticks; all roe deer were infested by I. ricinus. Nymphs (n = 1000), males (n = 1449) and females (n = 1000) of I. ricinus were analysed in pools of up to 10 ticks to detect both Borreliella and Borrelia DNA. The average number of I. ricinus per roe deer was similar in both areas, regardless of the life stage; although the percentage of Borreliella and Borrelia positive pools was higher in ticks collected from roe deer hunted in the plateau area, no significant differences were detected. Sequence analysis at the flagellin gene allowed the identification of four Borreliella species (Borreliella afzelii, Borreliella garinii, Borreliella lusitaniae and Borreliella valaisiana) and Borrelia miyamotoi in adult males; only B. valaisiana and B. miyamotoi were detected in nymphs and all females were negative. All Borreliella and Borrelia species found in roe deer were previously identified in questing I. ricinus collected in the same study area, although the prevalence was lower in the present study. The analysis of male I. ricinus ticks collected from roe deer gives a good estimation of Borreliella diversity in questing ticks. 相似文献
5.
Isidro Toro-Moyano Bienvenido Martínez-Navarro Jordi Agustí Caroline Souday José María Bermúdez de Castro María Martinón-Torres Beatriz Fajardo Mathieu Duval Christophe Falguères Oriol Oms Josep Maria Parés Pere Anadón Ramón Julià José Manuel García-Aguilar Anne-Marie Moigne María Patrocinio Espigares Sergio Ros-Montoya Paul Palmqvist 《Journal of human evolution》2013
The Orce region has one of the best late Pliocene and early Pleistocene continental paleobiological records of Europe. It is situated in the northeastern sector of the intramontane Guadix-Baza Basin (Granada, Andalusia, southern Spain). Here we describe a new fossil hominin tooth from the site of Barranco León, dated between 1.02 and 1.73 Ma (millions of years ago) by Electron Spin Resonance (ESR), which, in combination with paleomagnetic and biochronologic data, is estimated to be close to 1.4 Ma. While the range of dates obtained from these various methods overlaps with those published for the Sima del Elefante hominin locality (1.2 Ma), the overwhelming majority of evidence points to an older age. Thus, at the moment, the Barranco León hominin is the oldest from Western Europe. 相似文献
6.
Gláucia F. Cota Marcos R. de Sousa Andrea Laender Pessoa de Mendon?a Allan Patrocinio Luiza Siqueira Assun??o Sidnei Rodrigues de Faria Ana Rabello 《PLoS neglected tropical diseases》2014,8(4)
BackgroundVisceral leishmaniasis (VL) is an emerging condition affecting HIV-infected patients living in Latin America, particularly in Brazil. Leishmania-HIV coinfection represents a challenging diagnosis because the clinical picture of VL is similar to that of other disseminated opportunistic diseases. Additionally, coinfection is related to treatment failure, relapse and high mortality.ObjectiveTo assess the clinical-laboratory profile and outcomes of VL-HIV-coinfected patients using a group of non HIV-infected patients diagnosed with VL during the same period as a comparator.MethodsThe study was conducted at a reference center for infectious diseases in Brazil. All patients with suspected VL were evaluated in an ongoing cohort study. Confirmed cases were divided into two groups: with and without HIV coinfection. Patients were treated according to the current guidelines of the Ministry of Health of Brazil, which considers antimony as the first-choice therapy for non HIV-infected patients and recommends amphotericin B for HIV-infected patients. After treatment, all patients with CD4 counts below 350 cells/mm3 received secondary prophylaxis with amphotericin B.ResultsBetween 2011 and 2013, 168 patients with suspected VL were evaluated, of whom 90 were confirmed to have VL. In total, 51% were HIV coinfected patients (46 patients). HIV-infected patients had a lower rate of fever and splenomegaly compared with immunocompetent patients. The VL relapse rate in 6 months was 37% among HIV-infected patients, despite receiving secondary prophylaxis. The overall case-fatality rate was 6.6% (4 deaths in the HIV-infected group versus 2 deaths in the non HIV-infected group). The main risk factors for a poor outcome at 6 months after the end of treatment were HIV infection, bleeding and a previous VL episode.ConclusionAlthough VL mortality rates among HIV-infected individuals are close to those observed among immunocompetent patients treated with amphotericin B, HIV coinfection is related to a low clinical response and high relapse rates within 6 months. 相似文献
7.
Paul Palmqvist Mathieu Duval Antonio Diéguez Sergio Ros-Montoya Ma. Patrocinio Espigares 《Historical Biology》2016,28(6):734-752
Lozano-Fernández et al. (Lozano-Fernández I, Blain HA, López-García JM, Agustí J. 2014. Biochronology of the first hominid remains in Europe using the vole Mimomys savini: Fuente Nueva 3 and Barranco León D, Guadix-Baza Basin, south-eastern Spain. Hist Biol: Int J Paleobiol. doi:10.1080/08912963.2014.920015) recently published age estimates for two Late Villafranchian sites of Orce (Guadix-Baza basin, SE Spain), BL-D and FN-3, which provide some of the earliest evidence of human presence in Western Europe. The estimates were obtained from mean Lm1 values of the water vole Mimomys savini preserved in the sites and a couple of rectilinear equations derived in the Atapuerca TD section for site age on tooth length. However, this chronometric tool has problems that discourage its use in biostratigraphy, including: (1) the assumption of an orthogenetic trend of Lm1 increase during the evolution of the M. savini/Arvicola lineage; (2) the use of a chronology for the TD section not supported by original ESR data; (3) the discrepancies between the mean Lm1 values published for the TD levels and (4) the chronological ranges predicted when the standard deviations are used, which are exceedingly large as to be of value for biostratigraphic purposes. As a result, the pseudo numerical ages estimated for the Orce sites only add noise to the timing of the first human dispersal in Europe, which is based on a combination of results from well-established techniques such as palaeomagnetism, biostratigraphy and ESR. 相似文献
8.
Michael J. Jowers Fernando Amor Patrocinio Ortega Alain Lenoir Raphaël R. Boulay Xim Cerdá Juan A. Galarza 《Molecular ecology》2014,23(10):2529-2542
Gene flow is the main force opposing divergent selection, and its effects are greater in populations in close proximity. Thus, complete reproductive isolation between parapatric populations is not expected, particularly in the absence of ecological adaptation and sharp environmental differences. Here, we explore the biogeographical patterns of an endemic ant species, Cataglyphis floricola, for which two colour morphs (black and bicolour) coexist in parapatry throughout continuous sandy habitat in southern Spain. Discriminant analyses of six biometric measurements of male genitalia and 27 cuticular hydrocarbons reveal high differentiation between morphs. Furthermore, the low number of shared alleles derived from nuclear markers (microsatellites) between the morphs at their contact zone suggests the absence of recent gene flow. Mitochondrial DNA (COI) phylogenetic analysis and median‐joining networks show that the black morph is basal to the bicolour morph, with unique haplotypes recovered for each morph. Mismatch distribution analysis and Bayesian skyline plots suggest that they are undergoing different demographic changes, with the bicolour and black morphs at demographic equilibrium and expansion, respectively. Thus, our results show complete reproductive isolation between the two colour morphs as evidenced from genetic, chemical and morphological data. We suggest that these divergence events could be explained by historical vicariance during the Pleistocene, in which reproductive traits experienced strong divergent selection between the morphs initiating or culminating speciation. 相似文献
9.
Arias MS Martínez-Carrasco C León-Vizcaíno L Paz-Silva A Díez-Baños P Morrondo P Alonso F 《The Journal of parasitology》2012,98(4):754-759
Wild ruminants sharing pastures with domestic livestock are at risk of infection by liver trematodes. Detection of antibodies provides a very useful tool to gain more knowledge about the distribution of these parasites. Non-lethal methods are strongly encouraged for the analysis of the risk of infection among wild ruminants. A seroepidemiological survey was conducted to analyze exposure to hepatic trematodes ( Fasciola hepatica and Dicrocoelium dendriticum ) in wild ruminants from southern Spain. Blood samples were collected from 69 bovids (Mouflon + Spanish ibex) and 143 cervids (red deer + fallow deer) from Sierra de Cazorla, Segura and Las Villas Natural Park. The samples were analyzed using the excretory/secretory antigens of each trematode to determine the IgG response. All the animals were examined at necropsy for the presence of flukes, and the species, age, and gender of the animals were recorded. Fasciola hepatica were only observed in cervids (3%; 95% confidence interval [CI] = 2-8), while D. dendriticum specimens were recorded in 1% (0-8) of bovids and 4% (CI = 2-9) of the cervids. The IgG-seroprevalence against F. hepatica was significantly higher in the cervids. Statistical differences according to gender were observed. The bovids exhibited the greatest percentages of positive cases to D. dendriticum antigens, and the DdES-seroprevalence was related to age of the animals. When considering all the factors, the FhES-seroprevalence was initially distributed according to the type of ruminant (cervids), gender (male), and age (>2 yr). 相似文献
10.
Panadero R Fernández M Vázquez L López C Dacal V Cienfuegos S Díaz P Morrondo P Díez-Baños P 《Medical and veterinary entomology》2007,21(3):225-230
From March 2004 to March 2005, 862 bovine oesophagi were collected at weekly intervals from a slaughterhouse in Lugo in northwest Spain in order to establish the occurrence and chronobiology of the first-instar (L1) oesophageal phase of Hypoderma lineatum (De Villers) (Diptera: Oestridae) in different climatic areas from this region. First instars were observed in oesophagi from May to February (10 months/year). The annual prevalence was 18.2%. Maximum prevalence occurred in July and October. Prevalence in the coastal area was significantly lower than in the other areas. A total of 846 L1s were extracted from 120 infested oesophagi, 814 from the submucosa and 32 from external locations. The mean intensity of infestation was low (7.6 +/- 6.2); only 3.3% of the oesophagi harboured more than 30 L1s. Maximum numbers of L1/animal were observed in November and January. There were no differences in intensity of infection between climatic areas. The length of L1s ranged from 4.6 +/- 0.86 mm in May to 14.6 +/- 1.04 mm in January. The growth of larvae collected from external sites was parallel to those found in the submucosa. Mountainous areas produced the L1s of greatest length, followed by central and transition zones and, finally, coastal zones. To avoid adverse reactions resulting from the application of systemic insecticides, it will be necessary to adjust the time at which cattle are treated according to geographical or climatic area. 相似文献