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排序方式: 共有214条查询结果,搜索用时 78 毫秒
1.
Mutagenesis of human granulocyte colony stimulating factor 总被引:9,自引:0,他引:9
T Kuga Y Komatsu M Yamasaki S Sekine H Miyaji T Nishi M Sato Y Yokoo M Asano M Okabe 《Biochemical and biophysical research communications》1989,159(1):103-111
To define the structure-function relationship, we have made a number of mutants of human granulocyte colony-stimulating factor (hG-CSF) by in vitro mutagenesis. The results indicate that most of the mutations located in the internal and C-terminal regions of the molecule abolished the activity, whereas the mutants without N-terminal 4, 5, 7, or 11 amino acids retained the activity. N-terminal amino acids were also altered by cassette mutagenesis using a synthetic oligonucleotide mixture. Among them, KW2228, in which Thr-1, Leu-3, Gly-4, Pro-5 and Cys-17 were respectively substituted with Ala, Thr, Tyr, Arg and Ser, showed more potent granulopoietic activity than that of intact hG-CSF both in vitro and in vivo. 相似文献
2.
3.
Hiroshi Suzuki Hideaki Taguchi Kazuko Nishimura Makoto Miyaji Akira Nakamura Hironori Nakajima 《Mycopathologia》1988,104(1):7-17
Summary The authors succeeded in establishing a murine model of systemic candidiasis being disseminated from the primary gastrointestinal lesions caused by oral inoculation of Candida albicans. Using this model, an attempt was made for detecting the Candida antigen by enzyme-linked immunosorbent assay using avidin-biotin (AB-ELISA) from the serum of infected mice.Gastrointestinal candidiasis was formed in all of the 20 mice treated with the drugs (antibiotics, antineoplastic agents, hydrocortisone, etc.) and inoculated orally with C. albicans. Fourteen of these mice suffered from submucosal candidiasis, and C. albicans was cultured from the visceral organs in 12 of them. The assay by AB-ELISA was able to detect 1.0 ng/ml Candida mannan in the mouse serum. The Candida antigen was detected in the sera of 11 of the 14 mice with submucosal candidiasis. However, the antigen could not be detected in the sera of the 6 mice with intramucosal candidiasis.The assay by AB-ELISA is more sensitive and specific for the diagnosis of systemic candidiasis than other serological assays. 相似文献
4.
The relationship between paracoccidioidomycosis in ddY mouse and its estrous cycle was studied. Adult ddY mice of both sexes were used as experimental animals. Estrous cycle of female mice was examined before inoculation of Paracoccidioides brasiliensis yeast cells and mice were divided into 5 groups such as proestrus, estrus, metestrus-I, metestrus-II and diestrus. Each mouse was inoculated intravenously with 106
P. brasiliensis yeast cell units and sacrificed on day 28 after inoculation. Their internal organs were cultured, and in addition, their histopathologies were studied. As a result, there was no difference in the organ cultures among the male and the female mice of 5 groups. However, histopathologically, the female groups at estrus, metestrus-I and metestrus-II were affected more severely than the male group, and the susceptibility of the female mice to the fungus was closely related to their estrous cycles.Abbreviations BHI-D
brain heart infusion agar supplemented with 1.0% of anhydrous dextrose
- PAS
periodic acid-Schiff techniques
- PBS
phosphate buffered saline solution
- SD
standard deviation 相似文献
5.
Francisco Yegres Nicole Richard-Yegres Kazuko Nishimura Makoto Miyaji 《Mycopathologia》1991,114(2):71-76
Three strains of Cladosporium carrionii, two human isolates and one from a xerophilous plant, were used to study the effect of culture conditions in 106 newborn ddY mice. Growth in a complex medium (YPG) and a basal synthetic medium (BSM) was compared. Filamentous forms developed during static incubation while conidia were readily formed with shaking. Mice inoculated intraperitoneally were sacrified and autopsied after 4 weeks. Mortality was related only to sporulated exponential phase growing cells. Invasiveness ability was preserved in all experimental conditions. BSM medium that inhibited exopigment formation appeared more suitable than YPG to obtain intact cells for further studies.Biochemical and physiological alteration associated with shape changes during differentiation of vegetative cells into spores could play an important role in virulence of C. carrionii 相似文献
6.
Ken-ichi Miyaji 《Ecological Research》1986,1(3):303-322
In order to demonstrate in detail the relationship between the longevity and productivity of leaves within a canopy, a new
life table approach, the ‘bioeconomic life table’, was applied to the leaves of kidney bean plants (Phaseolus vulgaris L.) in relation to planting density and their position within the canopy.
The net photosynthetic rate for upper leaves under full daylight tended to decline gradually due to leaf senescence from about
20 days after leaf emergence, and for the lower leaves the decrease was very rapid due to both shading and senescence about
10 days after emergence.
Analysis of the survivorship curves and daily surplus production of leaves suggested that the lower and middle leaves, especially
the latter, survived without surplus production of dry matter after they had reached mean longevity, and while the upper leaves
at high density had a much shorter mean longevity, they had very large values of daily surplus production throughout the survival
period.
For the total foliage, the summed value of accumulated surplus production during the survival period was about five times
as large as the summed value of the dry weight of the dead leaves, regardless of planting density.
The daily rate of canopy leaf respiration was almost proportional to that of canopy gross photosynthesis for the various leaf
area indices of the canopy, so that there was no optimum leaf area index that maximized canopy daily surplus production. 相似文献
7.
Shinji Hosoi Mitsuo Satoh Hiromasa Miyaji Tatsunari Nishi Tamio Mizukami Mamoru Hasegawa Seiga Itoh Tatsuya tamaoki 《Cytotechnology》1995,19(1):1-10
Pro-UKS1 was designed as a thrombin-resistant derivative of pro-urokinase (pro-UK) by introducing a glycosylation site using site-directed mutagenesis. An expression plasmid for pro-UKS1, pMo1UKS1SEd1-5, was constructed and introduced into Namalwa KJM-1 cells (Hosoiet al., 1988), and cells resistant to G418 and Methotrexate (MTX) were obtained. Amongst them, the highest pro-UKS1 producer (resistant to 500 nM of MTX), clone 41-8, was selected and further characterized. Clone 41-8 was cultured in serum-free ITPSGF medium (Hosoiet al., 1988). Under the conventional conditions, the concentration of pro-UKS1 reached 26 g ml–1. Addition of glucose and tri-iodothyronine (T3) improved productivity, and the maximal productivity of pro-UKS1 was 67 g ml–1 day–1. In this conditioned medium, content of pro-UKS1 was above 80% of total proteins.Abbreviations BSA
bovine serum albumin
- dhfr
dihydrofolate reductase
- HEPES
4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid
- kb
kilobase pairs
- kDa
kilodaltons
- MTX
Methotrexate
- PBS
phosphate buffered saline
- pro-UK
pro-urokinase
- SDS-PAGE
sodium dodecyl sulfate-polyacrylamide gel electrophoresis
- T3
tri-iodothyronine
- Tween-PBS
phosphate buffered saline containing 0.05% Tween 80 相似文献
8.
The chief histopathological features found in patients with cryptococcosis are both a cystic (gelatinous) lesion and a granulomatous
reaction. These two tissue reactions are definitely different from each other, because a cyst is not accompanied with a significant
cellular response, while a granuloma is formed as a result of various cell reactions. Therefore, it is very interesting that
these two types of lesion can be observed in the same patient or in the same animal infected with Cryptococcus neoformans. From our previous paper (II) the authors reach such a thought that two steps may be required for the granuloma formation
against C. neoformans infection: first, of phagocytosis by sessile macrophages of C. neoformans and second is related to T-cell function. This experiment was done to verify that the granulomatous response against C. neoformans infection might occur easily in the organs rich in sessile macrophages as compared with those poor in them and a polysaccharide
capsule surrounding cryptococci may have effects to inhibit a migration of polymorphonuclear leucocytes or monocytes toward
C. neoformans. C. neoformans strain RIB 12 (serological type A, mating type α) was used in this experiment. After a culture of a brain heart infusion glucose agar slant at 37 C for 3 days, yeast cells
of the strain were harvested, and suspended in 1/15 M(pH7.4) sterile phosphate buffered saline solution. Infective inoculum was prepared by adjusting the number of the yeast cells
to 105, 106 or 5×106/0.2 ml in a hemacytometer. Fourty-two male mice strain ddY were divided into 3 groups consisting of 14 each and one group
was allotted to one of the cell suspensions. Each mouse was inoculated with 0.2 ml of the cell suspension into a tail vein
and one mouse from each group was sacrificed at adequate intervals. At necropsies the brain, thymus, lungs, heart, liver,
kidneys, spleen, pancreas, mesenteric lymph nodes, a part of the small intestine, testes and fat tissue were removed. From
these organs histopathological sections, stained with HE or by PAS, were prepared. To investigate effects of a polysaccharide
capsule to a migration of polymorphonuclear leucocytes or monocytes, double infections with C. neoformans and Aspergillus fumigatus, and an observation by the ‘Agar-Implantation method’ were done.
As results, granulomata were formed easily in the organs rich in macrophages or lymphocytes such as the liver, spleen, lymph
nodes, thymus, lungs, small intestine and fat tissue. On the contrary, in organs poor in the macrophages such as the brain,
heart, pancreas, kidneys, adrenal glands and testes, the chief histopathological feature was a cyst formation containing numerous
yeast cells. In the double infection, two types of lesions such as cysts and abscesses were observed in the sections of the
brain. The former occurred against C. neoformans infection and the latter, against A. fumigatus infection. Even though a cyst was very close to an abscess, polymorphonuclear leucocytes or monocytes were never induced
to C. neoformans. In the observation using the ‘Agar-Implantation method’, a severe cellular infiltration occurred to a perfect (teleomorphic)
state of C. neoformans and very weak response, to yeast cells with a polysaccharide capsule. The difference may be due to the existence of the capsule,
because a perfect state of C. neoformans is not surrounded by it. 相似文献
9.
Kazuki Miyaji Maki Kato Nobuyo Ohtani Mitsuaki Ohta 《Journal of applied animal welfare science : JAAWS》2013,16(4):355-362
The objective of this study was to evaluate the effects of diet on the feline stress response by measuring plasma and urinary cortisol. A study diet was developed with a unique combination of nutrients that supports the management of stressful situations. The specific formulation of the diet included alpha-casozepine, which is believed to have an anxiolytic effect, and tryptophan supplementation. Tryptophan is the precursor for the synthesis of the neurotransmitter serotonin. Twenty-one indoor cats were fed with the study diet (n = 10) or a control diet (n = 11) for 8 weeks, after which physiological responses were evaluated. The study diet significantly increased the ratio of plasma tryptophan to large neutral amino acids and decreased urinary cortisol concentrations after being consumed daily for 8 weeks, but there was no effect on plasma cortisol levels following a stressful event (veterinary examination and blood draw). Further studies, such as behavioral analyses, are needed to clarify the effects of the study diet. 相似文献
10.