全文获取类型
收费全文 | 173篇 |
免费 | 7篇 |
出版年
2022年 | 2篇 |
2019年 | 1篇 |
2018年 | 2篇 |
2017年 | 1篇 |
2016年 | 3篇 |
2015年 | 8篇 |
2014年 | 8篇 |
2013年 | 29篇 |
2012年 | 16篇 |
2011年 | 8篇 |
2010年 | 4篇 |
2009年 | 3篇 |
2008年 | 9篇 |
2007年 | 13篇 |
2006年 | 11篇 |
2005年 | 8篇 |
2004年 | 9篇 |
2003年 | 11篇 |
2002年 | 12篇 |
2001年 | 1篇 |
1999年 | 2篇 |
1998年 | 4篇 |
1995年 | 1篇 |
1991年 | 1篇 |
1990年 | 2篇 |
1989年 | 1篇 |
1988年 | 1篇 |
1983年 | 2篇 |
1982年 | 1篇 |
1981年 | 2篇 |
1980年 | 1篇 |
1978年 | 1篇 |
1976年 | 1篇 |
1973年 | 1篇 |
排序方式: 共有180条查询结果,搜索用时 109 毫秒
1.
Tatsuo Nakahara Makoto Hirano Takashi Matsumoto Toshihide Kuroki Yoshinori Tatebayashi Tetsuyuki Tsutsumi Kouji Nishiyama Hiroaki Ooboshi Kaoru Nakamura Hiroshi Yao Akio Shiraishi Michinori Waki Hideyuki Uchimura 《Neurochemical research》1990,15(6):609-611
DNA and RNA contents in 20 brain regions or nuclei of the rat were determined by a highly sensitive method using high-performance liquid chromatography with electrochemical detection. The high DNA and RNA contents were found in the hypothalamic nuclei, especially the median eminence-arcuate nucleus. These results may be available for the preparation of nucleic acids as the regional control. 相似文献
2.
Kohei Kawashima Eiji Watanabe Ken-ichi Isobe Michinori Ogura Ei-ichi Nagura Kazumasa Yamada Itsuro Sobue Kenji Mizoguchi Yasuhiko Ito Yoshiyuki Nagai Izumi Nakashima 《Cellular immunology》1982,67(2):279-286
During the course of immunization of (C3H × DBA/2)F1 mice (genotype H-2k/b) with L cell (H-2k/k)/L1210 leukemia cell (H-2d/d) hybrids and L1210 leukemia cells, some of them produced a good titer of anti-self-H-2 (H-2d) antibodies. Antigens recognized by this anti-self-H-2 antiserum were shown to be controlled by the H-2K-IA-IB-IJ-IE subregions of the H-2d but not H-2k nor H-2b haplotypes of parental as well as F1 origins and to have a tissue distribution identical to that of class 1 H-2 (H-2K/D) antigens. 相似文献
3.
Michinori Ichikawa Masashi Urayama Gen Matsumoto 《The Journal of membrane biology》1991,120(3):211-222
Summary The effects of calmodulin (CaM) antagonists (W-7, W-5, trifluoperazine, chlorpromazine, quinacrine, diazepam, propericyazine and carmidazolium) on the sodium and potassium channels were studied on the intracellularly perfused and voltage-clamped giant axon of the squid. It was found that the drugs are more potent blockers of the sodium current than of the potassium current. The drugs also reduce the sodium gating current. The blockage of the sodium and gating current can be explained by assuming that the drugs interact with the sodium gating subunit in one of its closed states. The site of action is probably the intracellular surface of the axolemma where presumably a Ca2+-calmodulin complex can be formed. 相似文献
4.
1. The extent of racemization and the coupling yield in peptide synthesis were studied under high dilution conditions. The azide method yielded the best results. 2. Five linear penta-peptide precursors related to gramicidin S were subjected to cyclization in order to study how the difference in the sequence influences the yield and the ratio of cyclic dimer to monomer. The azide with the sequence of -L -Pro-L -Val-L -Orn(Z)-L -Leu-D -Phe- afforded diZ-gramicidin S in a high yield of 63%. 3. Alternaria mali toxin III, a cyclotetradepsipeptide phytotoxin, was synthesized. The activated linear tetradepsipeptide containing a D -Dap(Z) (N3-Z-D -2,3-diaminopropionic acid) residue at the N-terminus afforded the cyclic precursor (53%). The Dap residue in the precursor was converted into a ΔAla residue by Hofmann degradation to give the desired product. 相似文献
5.
Michinori Nakamura 《Bioscience, biotechnology, and biochemistry》2013,77(5):398-405
The properties of UDPG → fructose transglucosylase, partially purified from immature soybeans or broad beans, were investigated. Magnesium ions had a stimulating effect on this enzyme. Evidence was presented to show that UDPG, the glucosyl group donor for sucrose synthesis, was regenerated from UDP and G-1-P in the presence of ATP. 相似文献
6.
Akihiro Matsuda Kohjiro Nagao Michinori Matsuo Noriyuki Kioka Kazumitsu Ueda 《Journal of neurochemistry》2013,126(1):93-101
High cholesterol turnover catalyzed by cholesterol 24‐hydroxylase is essential for neural functions, especially learning. Because 24(S)‐hydroxycholesterol (24‐OHC), produced by 24‐hydroxylase, induces apoptosis of neuronal cells, it is vital to eliminate it rapidly from cells. Here, using differentiated SH‐SY5Y neuron‐like cells as a model, we examined whether 24‐OHC is actively eliminated via transporters induced by its accumulation. The expression of ABCA1 and ABCG1 was induced by 24‐OHC, as well as TO901317 and retinoic acid, which are ligands of the nuclear receptors liver X receptor/retinoid X receptor (LXR/RXR). When the expression of ABCA1 and ABCG1 was induced, 24‐OHC efflux was stimulated in the presence of high‐density lipoprotein (HDL), whereas apolipoprotein A‐I was not an efficient acceptor. The efflux was suppressed by the addition of siRNA against ABCA1, but not by ABCG1 siRNA. To confirm the role of each transporter, we analyzed human embryonic kidney 293 cells stably expressing human ABCA1 or ABCG1; we clearly observed 24‐OHC efflux in the presence of HDL, whereas efflux in the presence of apolipoprotein A‐I was marginal. Furthermore, the treatment of primary cerebral neurons with LXR/RXR ligands suppressed the toxicity of 24‐OHC. These results suggest that ABCA1 actively eliminates 24‐OHC in the presence of HDL as a lipid acceptor and protects neuronal cells. 相似文献
7.
Yoshiro Kinoshita Michinori Misaka Shuhei Kubota Hisao Ishikawa 《Bioscience, biotechnology, and biochemistry》2013,77(11):1975-1981
A mixture of two thionocarbamates was subjected to the acid-catalyzed rearrangement. A sample of the reaction mixture was analyzed by glpc and resolved into four components. From the cross-over result, it has been concluded that the acid-catalyzed rearrangement of alkyl thionocarbamates into the isomeric thiolcarbamates proceeds by an intermolecular alkylating mechanism. This conclusion was supported by the detection of a transalkylated intermediate. 相似文献
8.
Critical examinations were made on the conditions for preparing the sugar solutions to be analyzed by ion-exchange chromatography of sugar-borate complexes by the method of Khym and Zill. A procedure was proposed which gave the best recovery of sugars with minimum hydrolysis of sucrose. By means of this procedure, sugar solutions were prepared from potato tubers which had been stored at a high (30°C) or low temperature (6°C). Results of the chromatographic separation and determination of component sugars showed that main sugars present in potato tubers were sucrose, glucose, and fructose. Maltose and pentoses could not be detected. The contents of sucrose, glucose, and especially, fructose were far greater in potatoes stored at a low temperature than in those stored at a high temperature. 相似文献
9.
Michinori Nakamura 《Bioscience, biotechnology, and biochemistry》2013,77(1):52-58
Effects of materials extracted from potatoes with hot 80% ethanol on the course of phosphorylase reactions were examined. These materials had no effect on phosphorylase activities as determined by the rate of liberation of inorganic phosphate from glucose-1-phosphate in the presence of a large amount of primer; however, in the presence of a small amount of primer or in its absence, these materials had significant effects on the liberation of inorganic phosphate and starch formation. These results suggested the presence of a small amount of primers in these materials. Evidence was presented against the participation of d-enzyme on the formation of starch when partially purified preparations of potato phosphorylase or crude extracts from potatoes were used as the enzyme. 相似文献
10.
Hajime Taniguchi Fumihiko Odashima Makoto Igarashi Yoshiharu Maruyama Michinori Nakamura 《Bioscience, biotechnology, and biochemistry》2013,77(8):2107-2115
A bacterium which can utilize potato starch granules as sole carbon source was isolated and identified as Bacillus circulans from its physiological and biochemical properties. Scanning electron microscopic observation of potato starch granules recovered from the culture broth revealed that granules were degraded gradually from their surface resulting in elongated granules with layered structures on their surface. This bacterium produced extracellular amylase which can digest potato starch granules in vitro. The amylase has a unique property in that it produces only maltohexaose from gelatinized starch in the early stage of the reaction. For the production of this amylase potato starch was found to be most effective while soluble sugars including gelatinized starch and maltose had little effect. 相似文献