全文获取类型
收费全文 | 8854篇 |
免费 | 871篇 |
国内免费 | 103篇 |
出版年
2022年 | 108篇 |
2021年 | 193篇 |
2020年 | 127篇 |
2019年 | 143篇 |
2018年 | 158篇 |
2017年 | 139篇 |
2016年 | 217篇 |
2015年 | 375篇 |
2014年 | 375篇 |
2013年 | 470篇 |
2012年 | 590篇 |
2011年 | 517篇 |
2010年 | 336篇 |
2009年 | 324篇 |
2008年 | 417篇 |
2007年 | 336篇 |
2006年 | 339篇 |
2005年 | 319篇 |
2004年 | 311篇 |
2003年 | 271篇 |
2002年 | 289篇 |
2001年 | 192篇 |
2000年 | 221篇 |
1999年 | 182篇 |
1998年 | 108篇 |
1997年 | 99篇 |
1996年 | 80篇 |
1995年 | 85篇 |
1994年 | 60篇 |
1993年 | 73篇 |
1992年 | 141篇 |
1991年 | 141篇 |
1990年 | 148篇 |
1989年 | 137篇 |
1988年 | 109篇 |
1987年 | 130篇 |
1986年 | 114篇 |
1985年 | 102篇 |
1984年 | 106篇 |
1983年 | 75篇 |
1982年 | 61篇 |
1981年 | 69篇 |
1980年 | 49篇 |
1979年 | 76篇 |
1978年 | 45篇 |
1977年 | 67篇 |
1975年 | 53篇 |
1974年 | 50篇 |
1973年 | 64篇 |
1971年 | 48篇 |
排序方式: 共有9828条查询结果,搜索用时 312 毫秒
1.
In this work, incorporation of plasmid DNA, pre-complexed with PEI, into polyelectrolyte multilayers has been studied to further develop platforms for local gene delivery. Polyplex embedding in synthetic and naturally degradable architectures was efficient for transfection of human hepato-cellular carcinoma cells. 相似文献
2.
Isolation of viral coat protein mutants with altered assembly and aggregation properties 总被引:1,自引:0,他引:1 下载免费PDF全文
A method was developed to screen bacteria for synthesis of mutant proteins with altered assembly and solubility properties using bacteriophage MS2 coat protein as a model self-associating protein. Colonies expressing coat protein from a plasmid were covered with an agarose overlay under conditions that caused the lysis of some of the cells in each colony. The proteins thus liberated diffused through the overlay at rates depending on their molecular sizes. After transfer of the proteins to a nitrocellulose membrane, probing with coat protein-specific antiserum revealed spots whose sizes and intensities were related to the aggregation state of coat protein. The method was employed in the isolation of assembly defective mutants and to find soluble variants of an aggregation-prone coat protein mutant. 相似文献
3.
4.
Thorsten Thye Genevieve Scarisbrick Edmund N. L. Browne Margaret Amanua Chinbuah John Gyapong Ivy Osei Ellis Owusu-Dabo Stefan Niemann Sabine Rüsch-Gerdes Christian G. Meyer Rolf D. Horstmann 《PloS one》2009,4(7)
The gene of Cytotoxic T Lymphocyte-associated Antigen 4 (CTLA4), a negative regulator of T lymphocytes, contains a single-nucleotide polymorphism (SNP) at position +6230A->G (ct60A->G), which has been found associated with several autoimmune diseases and appears to reduce T-cell inhibitory activity. In Ghana, West Africa, we compared the frequencies of CTLA4 +6230 A/G and 6 haplotype-tagging SNPs in 2010 smear-positive, HIV-negative patients with pulmonary tuberculosis (TB) and 2346 controls matched for age, gender and ethnicity. We found no difference in allele frequencies between cases and controls. However, +6230A and a distinct CTLA4 haplotype and a diplotype comprising the +6230A allele were significantly less frequent among cases with large opacities in chest radiographs compared to those with small ones (Pcorrected [cor] = 0.002, Pcor = 0.00045, P = 0.0005, respectively). This finding suggests that an increased T-cell activity associated with the CTLA4 +6230G allele contributes to pathology rather than to protection in pulmonary TB. 相似文献
5.
A total dosage up to 1 mg PGF2a as i.v. infusions of 10–40 μg/min. was investigated on patients with arrhythmias of several kinds. We found therapeutic effects in 5 of 6 patients with constant extrasystoles and in one patient with digitalis - induced partial AV-block respectively. In 3 of 4 patients with acute tachyarrhythmias the results were not convincing, probably due to a dosage not high enough. An increase of the diastolic stimulation threshold usually seen with other antiarrhythmics was not to be observed in 3 patients. The mechanism of action of PGF2a has not yet been clarified. 相似文献
6.
With enzyme histochemical methods, the distribution and the activities of various oxidative and hydrolytic enzymes in the apocrine glands of the general body surface of the domestic cat were investigated. The results obtained support the view that these glands are clearly active in function. On the whole, however, the relatively weak enzyme activities in the secretory portion of the glands point to only unimportant secretion production rates. 相似文献
7.
C Gerdemann C Eicken A Magrini H E Meyer A Rompel F Spener B Krebs 《Biochimica et biophysica acta》2001,1548(1):94-105
The amino acid sequences of two isozymes of catechol oxidase from sweet potatoes (Ipomoea batatas) were determined by Edman degradation of BrCN cleavage fragments of the native protein and by sequencing of amplified cDNA fragments. Sequence alignment and phylogenetic analysis of plant catechol oxidases revealed about 80% equidistance between the two I. batatas catechol oxidases and approximately 40--60% to catechol oxidases of other plants. When H(2)O(2) was applied as substrate the 39 kDa isozyme, but not the 40 kDa isozyme, showed catalase-like activity. The structure of the 40 kDa isozyme was modeled on the basis of the published crystal structure of the 39 kDa isozyme [T. Klabunde et al., Nat. Struct. Biol. 5 (1998) 1084]. The active site model closely resembled that of the 39 kDa isozyme determined by crystallography, except for a mutation of Thr243 (40 kDa isozyme) to Ile241 (39 kDa isozyme) close to the dimetal center. This residue difference affects the orientation of the Glu238/236 residue, which is thought to be responsible for the catalase-like activity of the 39 kDa isozyme for which a catalytic mechanism is proposed. 相似文献
8.
9.
10.