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排序方式: 共有76条查询结果,搜索用时 31 毫秒
1.
Regulation by phosphate of alkaline phosphatase in rat kidney 总被引:3,自引:0,他引:3
F Melani G Ramponi M Farnararo E Cocucci A Guerritore 《Biochimica et biophysica acta》1967,138(2):411-420
2.
Vanessa Castelli Francesco Melani Claudio Ferri Michele d'Angelo Mariano Catanesi Davide Grassi Elisabetta Benedetti Antonio Giordano Annamaria Cimini Giovambattista Desideri 《Journal of cellular biochemistry》2020,121(12):4862-4869
Oxidative stress is considered the common effector of the cascade of degenerative events in many neurological conditions. Thus, in this paper we tested different nutraceuticals in H2O2 in vitro model to understand if could represent an adjuvant treatment for neurological diseases. In this study, nutraceuticals bacopa, lycopene, astaxanthin, and vitamin B12 were used alone or in combination in human neuronal differentiated SH-SY5Y cells upon hydrogen peroxide-induced injury and neuroprotective, neuronal death pathways were analyzed. The nutraceuticals analyzed were able to protect H2O2 cytotoxic effects, through increasing cell viability and proteins involved in neuroprotection pathways and restoring proteins involved in cell death pathways. On this basis, it is possible to propose the use of these compounds as dietary supplement for the prevention or as adjuvant to the only symptomatic treatments so far available for neurodegenerative diseases. 相似文献
3.
Parathyroidectomy Improves Survival In Patients with Severe Hyperparathyroidism: A Comparative Study
Patricia Taschner Goldenstein Rosilene Motta Elias Lilian Pires de Freitas do Carmo Fernanda Oliveira Coelho Luciene Pereira Magalh?es Gisele Lins Antunes Melani Ribeiro Custódio Fábio Luiz de Menezes Montenegro Silvia Maria Titan Vanda Jorgetti Rosa Maria Affonso Moysés 《PloS one》2013,8(8)
Background and objectives
Secondary hyperparathyroidism (SHPT) in CKD is associated with an increased risk for mortality, but definitive data showing that parathormone control decreases mortality is still lacking. This study aimed to compare the mortality of patients with severe SHPT submitted to parathyroidectomy(PTX) with those who did not have access to surgery.Methods
This is a retrospective study in a cohort of 251 CKD patients with severe SHPT who were referred to a CKD-MBD Center for PTX from 2005 until 2012.Results
Most of our patients had indication of PTX, but only 49% of them had access to this surgical procedure. After a mean follow-up of 23 months, 72 patients had died. Non-survivors were older; more often had diabetes, lower serum 25 vitamin D and mostly had not been submitted to surgery. The relative risk of death was lower in the PTX patients (0.428; 95% CI, 0.28 to 0.67; p<0.0001). After adjustments, mortality risk was dependent on age (1.04; 95% CI, 1.01 to 1.07; p = 0.002), 25 vitamin D (0.43; 95% CI, 0.24 to 0.81; p = 0.006) and no access to PTX (4.13; 95% CI, 2.16 to 7.88; p<0.0001). Results remained the same in a second model using the PTX date as the study start date for the PTX group.Conclusions
Our data confirms the benefit of PTX on mortality in patients with severe SHPT. The high mortality encountered in our population is significant and urges the need to better treat these patients. 相似文献4.
5.
6.
This protocol describes a method for the dissection of egg chambers from intact Drosophila females and culture conditions that permit live imaging of them, with a particular emphasis on stage 9. This stage of development is characterized by oocyte growth and patterning, outer follicle cell rearrangement and migration of border cells. Although in vitro culture of egg chambers of later developmental stages has long been possible, until recently stage 9 egg chambers could only be kept alive for short periods, did not develop normally, and border cell migration failed entirely. We have established culture conditions that support overall egg chamber development including border cell migration in vitro. This protocol makes possible direct observation of molecular and cellular dynamics in both wild-type and mutant egg chambers, and opens the door to testing of pharmacological inhibitors and the use of biosensors. The entire protocol takes approximately 24 h while the preparation of egg chambers for live imaging requires only 15-20 min. 相似文献
7.
Iron-based phosphorus chelator: Risk of iron deposition and action on bone metabolism in uremic rats
Wander Barros do Carmo Brbara Bruna Abreu Castro Luísa Cardoso Manso Priscylla Aparecida Vieira do Carmo Clvis Antnio Rodrigues Melani Ribeiro Custdio Vanda Jorgetti Helady Sanders-Pinheiro 《Experimental biology and medicine (Maywood, N.J.)》2022,247(5):446
Phosphate chelators are frequently used in patients with chronic kidney disease (CKD). New iron-based chelators remain understudied and offer a promising therapeutic option for the control of bone and mineral disorders of chronic kidney disease (BMD-CKD). We assessed the effect of the phosphorus chelator, chitosan-iron III (CH-FeCl), compared to calcium carbonate (CaCO3) in BMD-CKD and the potential iron overload in uremic rats. Thirty-two animals were divided into four groups, namely the control, CKD, CKD/CH-FeCl, and CKD/CaCO3 groups. CKD was induced by adding 0.75% (4 weeks) and 0.1% (3 weeks) adenine to the diet. The chelators were administered from week 3 through week 7. The renal function, BMD-CKD markers, and histomorphometry of the femur were assessed at week 7. The CKD group showed a significant increase in creatinine (83.9 ± 18.6 vs. 41.5 ± 22.1 µmol/L; P = 0.001), phosphate (3.5 ± 0.8 vs. 2.2 ± 0.2 mmol/L; P = 0.001), fractional excretion of phosphorus (FEP) (0.71 ± 0.2 vs. 0.2 ± 0.17; P = 0.0001), and FGF23 (81.36 ± 37.16 pg/mL vs. 7.42 ± 1.96; P = 0.011) compared to the control group. There was no accumulation of serum or bone iron after the use of CH-FeCl. The use of chelators reduced the FEP (control: 0.71 ± 0.20; CKD/CH-FeCl: 0.40 ± 0.16; CKD/CaCO3 0.34 ± 0.15; P = 0.001), without changes in the serum FGF23 and parathyroid hormone levels. Histomorphometry revealed the presence of bone disease with high remodeling in the uremic animals without changes with the use of chelators. The CH-FeCl chelator was efficient in reducing the FEP without iron accumulation, thereby paving the way for the use of this class of chelators in clinical settings in the future. 相似文献
8.
Rebaudo R Melani R Balestrino M Cupello A Haglid K Hydèn H 《Neurochemical research》2000,25(4):541-545
Long term potentiation (LTP) was induced in the CA1 region of rat hippocampal slices by tetanization of the Schaffer collaterals. Local pretreatment of CA1 with serum of rabbits immunized against S-100 prevented the potentiation. However, treatment of the slices with a membrane permeant cAMP analogue, such as 8-Br-cAMP, could protect against the blocking effect of anti S-100 serum. We suggest that in the rat endogenous S-100b is involved in transduction mechanisms during LTP induction, via its ability to stimulate adenylate cyclase. Possible mechanisms of this action are discussed. 相似文献
9.
Rebaudo R Melani R Carità F Rosi L Picchio V Ruggeri P Izvarina N Balestrino M 《Neurochemical research》2000,25(11):1493-1495
Intracerebroventricular (ICV) administration of creatine increased cerebral phosphocreatine in normal rats by 67%, the highest increase so far reported in an in vivo model. We used osmotic minipumps (Alzet, Palo Alto, CA, USA) to administer creatine, 0.5mM, to the lateral ventricle at the rate of 10 l/h for 3 days. Brain phosphocreatine in saline-treated controls was 33 ± 17 M/g protein (mean ± SD, N = 9). In creatine-treated rats (0.5 mM for 3 days) such content was 55 ± 17 M/g protein (mean ± SD, N = 7). This difference is statistically significant (p = 0.02, t-test). The increase we found in cerebral phosphocreatine is of an order of magnitude comparable to the increase previously found in in vitro experiments, and may be effective in protecting brain tissue from ischemic damage. 相似文献
10.
Ibrahim MM de Angelis R Lima AS Viana de Carvalho GD Ibrahim FM Malki LT de Paula Bichuete M de Paula Martins W Rocha EM 《PloS one》2012,7(3):e33775