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Kuluev B. R. Gumerova G. R. Mikhaylova E. V. Gerashchenkov G. A. Rozhnova N. A. Vershinina Z. R. Khyazev A. V. Matniyazov R. T. Baymiev An. Kh. Baymiev Al. Kh. Chemeris A. V. 《Russian Journal of Plant Physiology》2019,66(5):694-706
Russian Journal of Plant Physiology - CRISPR/Cas genome editing of plants is realized in three basic variants, including knockout mutations as indels, insertion of alien DNA fragments, and base... 相似文献
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Baymiev An. Kh. Baymiev Al. Kh. Kuluev B. R. Shvets K. Yu. Yamidanov R. S. Matniyazov R. T. Chemeris D. A. Zubov V. V. Alekseev Ya. I. Mavzyutov A. R. Ivanenkov Ya. A. Chemeris A. V. 《Microbiology》2020,89(1):13-27
Microbiology - Specific amplification of nucleic acids is a convenient and quick alternative to the culture-based method of detecting bacterial cells. However, conventional PCR and other... 相似文献
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E. S. Saltykova A. A. Karimova A. R. Gataullin L. R. Gaifullina R. T. Matniyazov M. A. Frolova A. I. Albulov A. G. Nikolenko 《Applied Biochemistry and Microbiology》2016,52(5):553-557
High-molecular weight chitosan (200 kDa, 75% deacetylated) and N-succinoyl chitosan (300 kDa, 75% deacetylated) were shown to have a preadaptive effect and increase the lifespan of honeybees due to the induction of protective antioxidant and immune mechanisms. Chitosan with a molecular weight of 200 kDa had a fungistatic effect on a pathogenic fungus that causes ascospherosis, a disease of bee larvae and pupae. 相似文献
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Gerashchenkov G. A. Rozhnova N. A. Kuluev B. R. Kiryanova O. Yu. Gumerova G. R. Knyazev A. V. Vershinina Z. R. Mikhailova E. V. Chemeris D. A. Matniyazov R. T. Baimiev An. Kh. Gubaidullin I. M. Baimiev Al. Kh. Chemeris A. V. 《Molecular Biology》2020,54(1):24-42
Molecular Biology - CRISPR/Cas technology of genome editing is a powerful tool for making targeted changes in the DNA of various organisms, including plants. The choice of the precise nucleotide... 相似文献
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An. Kh. Baymiev R. S. Yamidanov R. T. Matniyazov D. K. Blagova Al. Kh. Baymiev A. V. Chemeris 《Molecular Biology》2011,45(6):904-910
A series of expression vectors containing TurboGFP and TurboRFP genes of fluorescent proteins under the control of the T5
phage constitutive promoter was created for a vital staining of nodule bacteria. These vectors were either obtained using
the broad host range pBBRI replicon for labeling of strains, where a marker gene was expressed from a transformed plasmid,
or they were prepared using the pRL765 gfp plasmid for labeling of strains via the introduction of genes of fluorescent proteins
into the bacterial chromosome. Transformation was shown to be the most convenient method of transfer of constructions into
cells of nodule bacteria, as there exists the possibility of spontaneous plasmid mobilization and, consequently, its transition
from cells of labeled strains into other soil bacteria if the mob locus is present in vectors needed for conjugation. Fluorescent labeled strains of Rhizobium sp., Mesorhizobium sp., Ensifer (Sinorhizobium) sp., Bradyrhizobium sp., Phyllobacterium sp., and Agrobacterium sp. were prepared using the obtained vector constructions. The suitability of the obtained strains for both in vivo and in
vitro experiments was demonstrated. 相似文献
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