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Aurelio Ciancio Mariantonietta Colagiero Laura Cristina Rosso Santos Nelida Murga Gutierrez Gaetano Grasso 《Mycoscience》2013,54(5):378-386
A new species of Hirsutella was isolated from unidentified mites on Petri plates inoculated with soil and root fragments collected from asparagus rhizosphere at Virú, Northern Peru. The fungus differs from other Hirsutella species by an envelope surrounding the conidium, conidia dimension and DNA sequences. In PDA cultures, the mycelium produced aerial hyphae with conidiogenous cells mainly at right angles, occasionally showing a secondary conidiophore. The solitary conidia are cymbiform, slightly apiculate, 5.0–6.0 × 3.0–4.0 μm. Phylogenetic analyses with partial rRNA and β-tubulin gene sequences confirmed the fungus as an Hirsutella (Ophiocordycipitaceae). Closest species shown by maximum likelihood and neighbor-joining trees were H. nodulosa and H. aphidis, from which the new species differs for conidium or conidiogenous cells dimensions, lack of synnemata and host type. A recombination event was also detected in the rRNA of the holotype strain, involving Ophiocordyceps sinensis as major parent and O. cochlidiicola as minor parent. A complement, inverted insertion was also found in its rRNA, involving part of the ITS2 and 5.8S regions, flanked by two short nucleotide arrays. Due to conidia dimension and phylogenetic position, the fungus is described as Hirsutella tunicata sp. nov. A review of mononematous Hirsutella species is provided. 相似文献
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Silvia Jane Lombardi Gianfranco Pannella Massimo Iorizzo Maria Victoria Moreno-Arribas Patrizio Tremonte Mariantonietta Succi Elena Sorrentino Vincenzo Macciola Massimo Di Renzo Raffaele Coppola 《World journal of microbiology & biotechnology》2018,34(11):161
In this study, the effect of sequential inoculation with non-Saccharomyces (Hanseniaspora guilliermondii) and Saccharomyces cerevisiae yeast on the distinctive characteristics of the Campanino white wine was investigated. For this purpose, three independent winemaking experiments were carried out on an industrial scale (batches A, B and C). In detail, the first one was carried out using the sequential inoculation technique while the other two, using a S. cerevisiae single-strain starter or no inoculation representing the control batches. Microbiological and chemical parameters and sensorial profiles of the wines were defined. Interestingly, the results showed that when sequential cultures (H. guilliermondii in a sequential mixture with S. cerevisiae) were used, a better wine aroma and quality was observed. More specifically, the wine obtained by sequential inoculation showed lower acetic acid values and enhanced volatile profiles than the wine from the control batches. Finally, sensorial analysis confirmed that the sequential cultures led to an improvement in wine flavour. Therefore, results suggest that the sequential inoculation using non-Saccharomyces and Saccharomyces yeast represents a biotechnological practice that can improve the quality features of traditional white wine. It has been shown for the first time that on an industrial scale H. guilliermondii could be used in sequential inoculum with S. cerevisiae in making white Campanino wine. 相似文献
Graphical abstract
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Air quality indices: a review 总被引:1,自引:0,他引:1
Antonella Plaia Mariantonietta Ruggieri 《Reviews in Environmental Science and Biotechnology》2011,10(2):165-179
National directives on air quality oblige nations to monitor and report on their air quality, allowing the public to be informed
on the ambient pollution levels. The last is the reason for the always increasing interest, demonstrated by the number of
publications on this topic in recent years, in air quality/pollution indices: since the concentration of individual pollutants
can be confusing, concentration measurements are conveniently transformed in terms of an air quality index. In this way, complex
situations are summarized in a single figure, letting comparisons in time and space be possible. In this paper we will give
an overview about the Air Quality/Pollution Indices proposed in literature and/or adopted by countries, trying also to categorize
them into homogeneous groups. For the classification different approaches can be followed. Since in real life exposure to
mixtures of chemicals occurs, with additive, synergistic or antagonistic effects, here we will distinguish between indices
that consider the conjoint effect of pollutants and indices only based on the actual most dangerous pollutant. This brief
review on air pollution indices shows, on one side, the wide interest in the problem, on the other, the lack of a common strategy
which allows to compare the state of the air for cities that follow different directives. The main differences between the
indices will be also described. 相似文献
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Anna Reale Tiziana Di Renzo Mariantonietta Succi Patrizio Tremonte Raffaele Coppola Elena Sorrentino 《World journal of microbiology & biotechnology》2011,27(2):237-244
Eleven sourdoughs from Molise region (Southern-Italy) were subjected to microbiological analyses in order to select predominant
lactobacilli species to be utilised as starter culture for bread production. A multiple approach was used, consisting of the
growth in different culture media, DGGE analysis, 16S rRNA gene sequencing and RAPD-PCR typing. Forty-three lactobacilli were
identified and four different species, facultatively or obligately heterofermentative lactobacilli, were found. Lactobacillus plantarum and Lactobacillus brevis represented the prevailing lactobacilli, while Lactobacillus casei and Lactobacillus paracasei ssp. paracasei were detected only in few samples. The use of different media demonstrated that there is no efficient medium for the study
of sourdoughs and the cultivation in different substrates remains the best tool to obtain a picture of lactic acid bacteria
population. DGGE and 16S rRNA gene sequencing allowed to obtain a reliable identification of strains, while RAPD-PCR resulted
a suitable method for typing lactobacilli at strain level. 相似文献
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Nicola Coppola Mariantonietta Pisaturo Caterina Sagnelli Evangelista Sagnelli Italo F. Angelillo 《PloS one》2014,9(4)
Background & aim
To compare the efficacy of pegylated-interferon (Peg-IFN) α-2a or α-2b and ribavirin given as dual therapy versus triple therapy (Peg-IFN and ribavirin plus boceprevir or telaprevir) in patients with HCV-1 chronic hepatitis naïve for anti-HCV therapy or relapsers to dual therapy in relation to the presence of constitutional, clinical and virological predictors of treatment response.Methods
Included in the meta-analysis were studies meeting these criteria: original data from randomized trials on the efficacy of dual versus triple therapy in therapy-naïve patients or relapsers; at least one primary outcome clearly defined: sustained virological response in patients with or without rapid virological response (RVR), with genotype 1a or 1b, low or high HCV load, IL28-B CC or non-CC genotype, mild or severe fibrosis; odds ratio estimates of relative risk (RR) and 95% confidence intervals; English language; and published up to the end of June 2013.Results
Seven original studies met the inclusion criteria, allowing a meta-analysis on 3,652 patients. Triple therapy was more effective than dual, regardless of IL-28B genotype, HCV sub-genotype, liver fibrosis, and baseline HCV load. In 1,045 patients who achieved RVR, SVR was more frequently achieved with dual therapy (RR = 1.11; p = 0.002) than triple. The same results were achieved when only the therapy-naïve patients were considered.Conclusions
Triple therapy provides a significantly higher SVR rate than dual therapy, but dual therapy obtains a significantly higher SVR rate in patients with RVR. The data stress the clinical importance of a 4-week lead-in phase in direct-acting antiviral-based treatment. 相似文献10.
Luca Dondini Orietta Lain Vera Vendramin Marisa Rizzo Davide Vivoli Marco Adami Michela Guidarelli Federica Gaiotti Francesco Palmisano Alessandra Bazzoni Donato Boscia Filippo Geuna Stefano Tartarini Paola Negri Mariantonietta Castellano Vito Savino Daniele Bassi Raffaele Testolin 《Molecular breeding : new strategies in plant improvement》2011,27(3):289-299
Sharka is a severe apricot viral disease caused by the plum pox virus (PPV) and is responsible for large crop losses in many countries. Among the known PPV strains, both PPV-D (Dideron) and PPV-M (Marcus) are virulent in apricot, the latter being the most threatening. An F1 apricot progeny derived from Lito, described in the literature as resistant, crossed to the susceptible selection BO81604311 (San Castrese × Reale di Imola) was used to study the genetic control of resistance to PPV. A population of 118 individuals was phenotyped by inoculating both PPV-D and PPV-M strains in replicated seedlings and scored for 3 years. An additional set of 231 seedlings from the same cross was also phenotyped for 2 years. SSR-based linkage maps were used for quantitative trait locus (QTL) analysis. A major QTL of resistance to both PPV-M and PPV-D strains was found in the top half of the Lito linkage group 1, where a QTL was previously described in Stark Earli-Orange, the donor of Lito resistance. The LOD score was considerably enhanced when the recovery of plants from infection was taken into account. The results obtained in Lito were compared with those observed in a second apricot cross progeny (Harcot × Reale di Imola) in which QTL of resistance to sharka were also mapped in the same linkage group 1 for both PPV strains. Several models of resistance to sharka disease are discussed considering the segregation frequencies, the QTL alignment in the two maps and the information gathered from the literature. 相似文献