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排序方式: 共有65条查询结果,搜索用时 31 毫秒
1.
Characterization of rco-1 of Neurospora crassa, a pleiotropic gene affecting growth and development that encodes a homolog of Tup1 of Saccharomyces cerevisiae. 总被引:2,自引:1,他引:1 下载免费PDF全文
C T Yamashiro D J Ebbole B U Lee R E Brown C Bourland L Madi C Yanofsky 《Molecular and cellular biology》1996,16(11):6218-6228
2.
Rco-3, a Gene Involved in Glucose Transport and Conidiation in Neurospora Crassa 总被引:2,自引:0,他引:2 下载免费PDF全文
Macroconidiation in Neurospora crassa is influenced by a number of environmental cues, including the nutritional status of the growing organism. Conidia formation is normally observed when the fungus is exposed to air. However, carbon limitation can induce conidiation in mycelia submerged in an aerated liquid medium. A mutant was previously isolated that could conidiate in submerged culture without imposing nutrient limitation and the gene responsible for this phenotype (rco-3) has now been cloned. RCO3 exhibits sequence similarity to members of the sugar transporter gene superfamily, with greatest similarity to glucose transporters of yeast. Consistent with this structural similarity, we find that glucose transport activity is altered in the mutant. However, growth of the mutant in media containing alternate carbon sources does not suppress conidiation in submerged culture. The properties of the mutant suggest that RCO3 is required for expression of glucose transport activity, glucose regulation of gene expression, and general carbon repression of development. 相似文献
3.
N Madi G Steiger J Estreicher J A Schifferli 《Journal of immunology (Baltimore, Md. : 1950)》1992,148(3):723-728
C3b-coated immune complexes (IC) adhere to complement receptor 1 (CR1) on human E in the circulation. E from AIDS patients have an acquired low CR1 number. To study immune adherence and IC elimination in AIDS, radiolabeled hepatitis B surface Ag/antibody complexes were injected i.v. in six AIDS patients and in 14 healthy controls. The binding of IC to E was reduced in AIDS patients (mean binding 2 min after injection: 24.9 +/- 13.3%) compared with healthy individuals (63 +/- 3.7%) (p = 0.0005). The low binding correlated directly with the number of CR1/E and to the capacity of these E to bind IC in vitro. During the first 15 min disappearance of IC was faster in AIDS patients than in normal subjects and correlated with CR1 number. Thereafter, elimination was very slow in AIDS patients, which suggested that a fraction of IC might be released back into the circulation similarly to what has been observed for C3b-coated E. When the data were analyzed with a mathematical model allowing for such release to occur, five of six AIDS patients had a high release rate compared with little or no release in normal individuals (p less than 0.001). Thus, low CR1 on E is responsible for defective immune adherence, and might determine abnormal disappearance of IC from the circulation as well. 相似文献
4.
Plant Cell, Tissue and Organ Culture (PCTOC) - Date palm (Phoenix dactylifera L.) micropropagation still faces many problems, such as reduced growth and development of callus, low multiplication... 相似文献
5.
6.
Li Y Sagar MB Wassler M Shelat H Geng YJ 《Biochemical and biophysical research communications》2007,357(1):157-161
Over-consumption of ethanol (EtOH) represents a major health problem. This study was to test the cytotoxicity of EtOH in cardiac stem cells or myoblasts, and the potential protective effect of apolipoprotein-J (ApoJ), a stress-responding, chaperone-like protein in high-density lipoprotein, on EtOH-injured cardiac myoblasts. In culture, EtOH-exposed canine fetal myoblasts underwent apoptosis in a concentration- and time-dependent manner. Expression ApoJ by cDNA transfection markedly reduced EtOH-induced apoptosis in the cells. ApoJ expression also restored partially the mitochondrial membrane potential and prevented the release of cytochrome-c from mitochondria into cytoplasma. Thus, ApoJ serves as a cytoprotective protein that protects cardiac stem cells against EtOH cytotoxicity. 相似文献
7.
Tomislav Bedeković Nina Lemo Ivana Lojkić Ana Beck Mirko Lojkić Josip Madić 《Acta veterinaria Scandinavica》2011,53(1):1-4
One hundred and thirty three "wild" muskoxen, 81 of which of known body mass, were successfully immobilized using etorphine (M99), and xylazine (Rompun®), delivered by use of a dart gun. A dose of 0.05 mg/kg M99, supplemented by 0.15 mg/kg Rompun was found to be very effective. This dose is much higher than currently recommended e.g. by Handbook of Wildlife Chemical Immobilization. 相似文献
8.
Ferreira MS Xavier Mda P Tinga AC Rose TL Fumian TM Fialho AM de Assis RM Carvalho Costa FA de Oliveira SA Leite JP Miagostovich MP 《PloS one》2012,7(3):e33754
This 15-year study aimed to determine the role of the main viruses responsible for acute infantile gastroenteritis cases in a day care center in the city of Rio de Janeiro, Brazil. From 1994 to 2008, 539 fecal samples were obtained from 23 outbreaks as well as sporadic cases that occurred in this period. The detection of Rotavirus group A (RVA), norovirus (NoV) and astrovirus (AstV) was investigated both by classical and molecular methods of viral detection. RVA was detected by enzymatic immune assay and/or polyacrylamide gel electrophoresis and genotyped by using semi-nested multiplex PCR. NoV and AstV were subsequently tested by real time PCR in all RVA-negative samples and genotyped throughout genome sequencing. Three protocols for molecular characterization of NoV nucleotide sequencing were performed with the partial nucleotide sequencing of genomic regions known as region B (polymerase gen), C and D (capsid gen).Viruses were identified in 47.7% (257/539) of the cases, and the detection rates of RVA, NoV and AstV in16.1% (87/539), 33.4% (151/452), and 6.3% (19/301), respectively. Most gastroenteritis cases were reported in autumn and winter, although NoV presented a broader monthly distribution. Viruses' detection rates were significantly higher among children aged less than 24 months old, although NoV cases were detected in all age groups. RVA genotypes as G1P[8], G9P[8], G2P[4], G3P[8] and G1+G3P[8] and RVA was no longer detected after 2005. NoV characterization revealed genotypes variability circulating in the period as GI.2, GI.3, GI.8 GII.2, GII.3, GII.4, GII.4 variants 2001 and 2006b, GII.6, GII.7, GII.12 and GII.17. AstV genotypes 1, 2, 4 and 5 were also characterized. Those data demonstrate the impact of NoV infection in cases of infantile gastroenteritis, surpassing RVA infection responsible for high morbidity rate in children under five years old. 相似文献
9.
S. Messaoudi A. Madi H. Prévost M. Feuilloley M. Manai X. Dousset N. Connil 《Anaerobe》2012,18(6):584-589
Lactobacillus salivarius SMXD51 was previously isolated from the cecum of a Tunisian poultry and found to produce a bacteriocin-like substance highly active against the foodborne pathogen Campylobacter jejuni. The aim of this study was to examine some probiotic properties of the strain: acid and bile tolerance, capacity of adhesion, stimulation of immune defences (IL-6, IL-8, IL-10 and β-defensin 2), and modulation of the barrier integrity. The results showed that L. salivarius SMXD51 can tolerate gastrointestinal conditions (acid and bile), adhere to intestinal cells and stimulate the immune system. The bacterium strengthened the intestinal barrier functions through the increase of the transepithelial electrical resistance (TEER) and reinforcement of the F-actin cytoskeleton. One hour pretreatment with L. salivarius SMXD51 protected against Pseudomonas aeruginosa PAO1-induced decrease of TEER and damage of the F-actin cytoskeleton. Our results highlight that L. salivarius SMXD51 fulfils the principle requirements of an efficient probiotic and may be seen as a reliable candidate for further validation studies in chicken. 相似文献
10.
Aggregation of the root-inhabiting, asymbiotic N-fixingAzospirillum brasilense Cd (ATCC-29729), was studied. Aggregation occurred towards the end of the exponential phase and during the stationary phase. More aggregates were formed in media supplemented with organic acids than in those containing sugars as a sole carbon source. Maximum growth with no aggregation was obtained in a medium containing both fructose and malate as carbon sources. Aggregation was increased by poly-L-lysine and carbodiimide as well as by increasing the C/N ratio and decreasing combined nitrogen in the growth medium. Aggregates were stable at pH levels of >8 and <6, but dispersed at pH 7.1. Treatment of Azospirillum with NaEDTA resulted in loss of both aggregative capacity and the ability of adsorb to wheat roots without losing cell viability. When extracted bacteria were suspended in their dialysed NaEDTA extract, both their aggregative and adsorptive capacities were restored.The dialysed NaEDTA extract agglutinated bacterial cells and red blood cells, especially of type O. When the extract was run through a sepharose gel, it separated into three main fractions, of which only one showed agglutinating capacity. Gel electrophoresis of this fraction revealed a single band (MW 97,000) which reacted positively to Schiff's reagent and Coomassie brilliant blue R-250, typical to a glycoprotein. Bacterial agglutination by this fraction was strongly inhibited by D-glucose, melibiose and -metyl glucoside. No evidence as to the involvement of cellulose fibrils in aggregation was found. It is suggested that glycoprotein(s) and glucose residues located on the outer surface of the cells are involved in aggregation of Azospirillum. 相似文献