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1.
Osteogenesis imperfecta due to recurrent point mutations at CpG dinucleotides in the COL1A1 gene of type I collagen 总被引:7,自引:3,他引:4
Charles J. Pruchno Daniel H. Cohn Gillian A. Wallis Marcia C. Willing Barbra J. Starman Xiaoming Zhang Peter H. Byers 《Human genetics》1991,87(1):33-40
Summary Most individuals with osteogenesis imperfecta (OI) are heterozygous for dominant mutations in one of the genes that encode the chains of type I collagen. Each of the more than 30 mutations characterized to date has been unique to the affected member (s) of the family. We have determined that two individuals with a progressive deforming variety of OI, OI type III, have the same new dominant mutation [1(I)gly154 to arg] and that two unrelated infants with perinatal lethal OI, OI type II, share a second new dominant muation [1(I)gly1003 to ser]. These mutations occurred at CpG dinucleotides, in a manner consistent with deamination of a methylated cytosine residue, and raise the possibility that CpG dinucleotides are common sites of recurrent mutations in collagen genes. Further, these findings confirm that the OI type-III phenotype, previously thought to be inherited in an autosomal recessive manner, can result from new dominant mutations in the COL1A1 gene of type-I collagen. 相似文献
2.
二核苷酸重复多态性的非同位素检测及其在基因诊断中的应用 总被引:9,自引:1,他引:8
本文报道了一种检测二核苷酸重复多态性的简便的非同位素法,利用重复序列两侧的特异引物进行PCR扩增,产生的等位片段在薄层变性聚丙烯酰胺凝胶电泳上分离,再用灵敏的银染法显色。该方法不需要标记PCR产物,简便、快速,分辨率可达1bp,并可用多对引物同时进行多重PCR分析。用此方法对DMD家系成员dystrophin基因的5'-脑型外显子止游区和3'-非翻译区的两个(CA)。位点进行了扩增片段长度多态性分 相似文献
3.
腹腔注射链脲佐霉素(65mg/kg)诱发Wistar大鼠糖尿病。糖尿病发病4周后,向饲料中加尼群地平(30mg·kg-1·d-1)。结果表明,糖尿病4周时大鼠心室舒张功能首先受损,8周后心室舒张和收缩功能均明显受累。尼群地平处理对糖尿病大鼠的心肌收缩性有一定的改善作用。提示尼群地平对大鼠糖尿病性心肌病有一定有益作用。 相似文献
4.
5.
Xiaoming Li Xiaopeng Duan Jiawei Qiao Shilin Li Yunhao Cai Jianqi Zhang Yuan Zhang Xiaotao Hao Yanming Sun 《Liver Transplantation》2023,13(1):2203044
The power conversion efficiencies (PCEs) of all-polymer solar cells (all-PSCs) have already exceeded 17%. However, the limited absorption range of an all-polymer system results in significantly reduced short-circuit current density (Jsc), which eventually influences the PCE improvement. To broaden the light absorption of polymer acceptors, herein, benzotriazole is introduced in the core unit of small molecule acceptors and thus two narrow-bandgap polymer acceptors named PTz-BO and PTz-C11 featuring the same molecular backbone and different side-chain length are synthesized. Compared with PTz-C11, the PTz-BO based-all PSCs deliver a slightly reduced Jsc, a large open-circuit voltage (Voc) and a low voltage loss below 0.50 V. Moreover, ternary all-PSCs are constructed by introducing PTz-C11 as a guest component. Benefiting from the reduced recombination, improved exciton generation and dissociation, and balanced charge transport, a high efficiency of 16.58% is obtained for the ternary all-PSCs, with a high Jsc over 25 mA cm−2 without sacrificing the Voc. Such result represents the highest efficiency reported for benzotriazole-based all-PSCs in the literature thus far. This work demonstrates the great potential of benzotriazole for the synthesis of efficient narrow-bandgap polymer acceptors. 相似文献
6.
原生质体电融合酵母多倍体生理特性的研究 总被引:5,自引:0,他引:5
对原生质体电融合技术获得的11株酵母多倍体融合株,进行了一系列生理特性的分析比较.结果发现,在电融合过程中,融合株的细胞体积及DNA含量并不随着核倍性呈线性递增.而是有其特殊的变化规律。当糖化酵母sta1、sta2和sta3在细胞核中各自纯合时,明显表现出表达的剂量效应。其中sta1、sta2纯合的融合株.在YEPS培养中,GA分泌可被淀粉大量诱导,表现出一定的二次生长特性。从分析结果推测,在亲株5301-14D及HU-TY—1A中可能有对GA分泌不利的因素。 相似文献
7.
四川55种鱼生活史型的研究 总被引:1,自引:0,他引:1
四川55种鱼生活史型的研究刁晓明,罗一兵李波(西南农业大学水产系,重庆630716)(重庆大学计算机系,630000)LifeHistoryPatternsof55FishSpeciesinSichuan¥DiaoXiaoming;LuoYibing... 相似文献
8.
本文首次研究了田菁蛋白胨一般化学成份、氨基酸组份、肽链平均长度、微量元素、维生素以及红外、紫外光谱等。本文还将田菁蛋白胨氨基酸、红外光谱等和英国大豆蛋白胨作了比较。研究结果附图表于后。 相似文献
9.
A new method for estimating gross phosphorus mineralization and immobilization rates in soils 总被引:7,自引:1,他引:6
Phosphorus availability in soils is controlled by both the sizes of P pools and the transformation rates among these pools. Rates of gross P mineralization and immobilization are poorly known due to the limitations of available analytical techniques. We developed a new method to estimate P transformation rates in three forest soils and one grassland soil representing an Alfisol, an Ultisol, and Andisol, and a Mollisol. Three treatments were applied to each soil in order to separate the processes of mineral P solubilization, organic P mineralization, and solution P immobilization. One set of soils was retained as control, a second set was irradiated with -rays to stop microbial immobilization, and a third was irradiated and then autoclaved, also stop phosphatase activity. All three sets of samples were then incubated with anion exchange resin bags under aerobic conditions. Differences in resin P among the three treatments were used to estimate gross P mineralization and immobilization rates. Autoclaving did not affect resin-extractable P in any of the soils. Radiation did not alter resin-extractable P in the forest soils but increased resin-extractable P in the grassland soil. This increase was corrected in the calculation of potential P transformation rates. Effects of radiation on phosphatase activity varied with soils but was within 30% of the original values. Rates of P gross mineralization and immobilization ranged from 0.6–3.8 and 0–4.3 mg kg-soil-1 d-1, respectively, for the four soils. The net rates of solubilization of mineral P in the grassland soil were 7–10 times higher than the rates in forest soils. Mineralization of organic P contributed from 20–60% of total available P in the acid forest soils compared with 6% in the grassland soil, suggesting that the P mineralization processes are more important in controlling P availability in these forest ecosystems. This new method does not require an assumption of equilibrium among P pools, and is safer and simpler in operation than isotopic techniques. 相似文献
10.
温室气体大量排放导致的全球变暖是最为关注的环境问题之一,这会直接影响植物的生长与发育进而影响群落组成乃至生态系统的结构和功能。水分利用效率作为植物叶片通过光合作用调节水分生理过程的指标,是联系生态系统碳循环与水循环关系的关键,反映了植被生态系统对立地环境快速调整和资源变化的适应策略,是当前全球变化研究中的重点。植物叶片碳稳定同位素比值(δ13C)是反映植物长期水分利用效率的关键指标,但全球气候变暖对植物叶片δ13C值的影响仍存在较大争议。该研究利用Meta分析整合全球范围内51份相关研究文献中的371组数据,较为系统地评估模拟增温对植物叶片δ13C及其生理生态指标的影响。结果表明,模拟增温能够使叶片δ13C值显著升高0.6%(P<0.001),同时对叶片呼吸速率Rd、气孔导度Gs、净光合作用速率Pn、碳C的效应值分别为0.237、0.062、-0.140、-0.019 (P<0.05)。模拟增温处理在增强植物光合作用的同时提高了叶片呼吸速率(Rd),导致光合产物不断被消耗、叶片碳(C)降低,最终使叶片Pn降低并且叶片δ13C产生富集现象。通过进一步对影响因素分析发现,叶片δ13C值对增温的响应主要受增温时间、高程和年均气温等控制(相对重要性指数分别为1.00、0.97和0.92)。另外,模拟增温时选用不同的增温模式对叶片δ13C值也具有显著不同的影响,采用红外线加热、土柱置换和电缆增温等方法对叶片δ13C值具有正向促进作用(效应值分别为0.70、0.44和0.35),而采用遮阳屏与开顶箱增温等方法具有负向作用(效应值分别为-0.17和-0.09)。研究结论对于深入理解全球变化背景下植物水分利用的响应特征具有重要的理论意义,以期为今后该领域的植物生长研究提供理论依据和有效支撑。 相似文献