溶血磷脂酸在皮肤损伤疾病中的作用及其分子机制

皮肤作为人体最大器官覆盖于全身,能阻挡有害物质的侵入,保护人体内环境稳态,参与人体代谢过程。皮肤损伤、炎症和纤维化等,都会导致皮肤屏障功能的减退,影响正常的生命活动。溶血磷脂酸(lysophosphatidic acid,LPA)是十分活跃的磷脂信号分子,参与多种生理和病理生理过程。LPA是维持体内平衡所必需的生物活性脂质介质,在皮肤中通过不同的信号通路发挥多功能磷脂信使作用。本文综述了皮肤中溶血磷脂酸受体(lysophosphatidic acid receptor,LPA1-6)及其细胞信号通路的作用及机制,综述了LPA在皮肤创面愈合、皮肤瘢痕、皮肤黑色素瘤、硬皮病、皮肤瘙痒、过敏性皮炎、皮肤屏障、皮肤疼痛,皮肤毛发生长中的作用及分子机制,有助于了解LPA在皮肤中的生理和病理生理作用。深入研究LPA的作用机制将有助于挖掘其在皮肤治疗中的作用,开发以LPA为靶点的药物。     

心不甘中甾体皂甙元的分离和结构鉴定(2)

自心不甘(Tupistra aurantiaca Wall et Backer)根的醋酸乙酯萃取物经硅胶柱层析分离除可得到1β、2β、3β、4β、5β、7α-hexahydroxyspirost-25(27)-en-6-one外,还得到7个游离的甾体皂甙元A—G,其中A及B分别为3-epiruscogenin及3-epi-neoruscogenin,F为△~(25(27))-pentrogenin(6)、C、D和E系新化合物,经IR、MS、~1H NMR及~(13)C NMR谱鉴定分别推定为ranmogenin A(3)、B(4)和C(5)(兰茂甙元甲、乙和丙)。     

心不甘中甾体皂甙元的分离和结构鉴定(3)

本文报告心不甘(Tupistra aurantiaca Wall et Backer)根的粗甙经盐酸水解后的总甙元中分离的甙元E和F的结构鉴定。甙元F经鉴定为△~(25(27))-pentrogenin(2)。甙元E(ranmo-genin D)为新化合物,其结构经IR、MS、~1H NMR和~(13)C NMR谱分析推定如(1)所示。     

Platinum-supported Bilayer Lipid Membranes modified by ferrocene and its derivatives

The biferrocene-containing Schiff base complexes (1) and (2) were synthesized and characterized by elemental analyses and spectral data. The Pt-supported Bilayer Lipid Membranes (BLMs) modified by ferrocene and its derivatives were studied by cyclic voltametry (CV) and the electrochemical properties of this system are reported. The oxidation mechanism of electrocatalysis of ascorbic acid on the Pt-supported BLMs is discussed.     

HPV感染对阴道微生态炎症反应的影响

目的探究HPV感染与阴道微生态、炎症反应的关系。方法选取2017年11月至2018年11月在我院治疗的70例HPV感染患者(研究组),随机选取同一时期来我院进行体检的健康者70例(对照组)。治疗前后分别对研究组和对照组研究对象进行HPV检测、阴道分泌物检查以及炎症反应检查,观察并记录两组研究对象的情况。结果治疗前研究组患者HPV感染情况[高危型HPV感染31例(44.3%),低危型HPV感染为39例(55.7%)]明显高于对照组[高危型HPV感染2例(2.8%),低危型HPV感染5例(7.1%)](t=38.314 0、33.345 0,均P0.05);研究组患者阴道微生态(乳杆菌为3 000±50、厌氧菌为1 050±100、需氧菌为800±65)明显低于对照组(乳杆菌为5 114±222、厌氧菌为2 334±100、需氧菌为1 500±100)(t=15.395 1、14.521 1、16.126 0,均P0.05);研究组阴道炎症发病情况[阴道毛滴虫阴道炎29例(41.43%)、念珠菌阴道炎为22例(31.43%)、细菌性阴道炎19例(27.14%)]明显多于对照组[阴道毛滴虫阴道炎11例(15.71%)、念珠菌阴道炎9例(12.86%)、细菌性阴道炎8例(11.43%)](χ~2=11.340 0、7.002 0、5.552 0,均P0.05)。结论临床上HPV感染很常见,患者多伴发阴道炎症,HPV感染患者阴道正常菌群变少,阴道微生态环境紊乱。     

土著入侵种黄帚橐吾的替代防控展望

黄帚橐吾是青藏高原高寒草场中分布最广、危害极大的优势毒草,严重影响了该地区群落结构和草地畜牧业的发展。在介绍土著入侵种和替代防控概念及相关研究进展的基础上,针对黄帚橐吾目前缺乏高效经济生态防控措施的现状,结合国内外对入侵植物进行替代防控的经验与原则,提出了对黄帚橐吾进行替代防控的生态防控设想和黄帚橐吾替代植物选用的基本原则,初步划定了防控黄帚橐吾的替代植物范围。通过引种或者驯化乡土草种,在青藏高原培育禾本科、豆科植物或者建植禾豆混播人工草地,使其具备替代防控黄帚橐吾的潜力。     

High nitrogen inhibits photosynthetic performance in a shade-tolerant and N-sensitive species Panax notoginseng

Photosynthesis Research - Nitrogen (N) is a primary factor limiting leaf photosynthesis. However, the mechanism of high-N-driven inhibition on photosynthetic efficiency and photoprotection is still...     

会仙喀斯特湿地三种典型植物叶片碳同位素(δ13C)特征及其指示意义

为探讨会仙喀斯特湿地不同生长环境下植物叶片碳(C)、氮(N)、磷(P)、稳定碳同位素(δ~(13)C)特征及其生态学指示意义,该文以挺水植物芦苇、浮水植物水葫芦和沉水植物金鱼藻为研究对象,分析了三种典型不同生活型植物叶片的δ~(13)C特征及种间和微生境的差异,并基于植物碳同位素与碳酸酐酶显著正相关的二端元模型,估算了植物利用光合作用固定的碳酸氢根离子(HCO3-)的碳量。结果表明:(1)三种植物叶片δ~(13)C的变化范围为-28.47‰～-21.69‰,平均值为-24.83‰,不同生活型植物间δ~(13)C存在差异,金鱼藻水葫芦芦苇。(2)植物δ~(13)C值与叶片C、N和P元素含量间呈显著正相关,与C/N、C/P和N/P呈显著负相关关系,与底泥的有机质、速效氮、总氮、速效磷和总磷含量呈显著正相关。(3)会仙喀斯特湿地三种不同生活型植物叶片N/P平均值为10.34,表现出植物受N、P共同影响的特征。(4)δ~(13)C的变化特征,揭示了三种水生植物可能通过增加磷利用效率来促进低水分利用率环境下的碳的合成,通过提高植物水分利用效率的策略来代偿较低的氮素利用效率。(5)芦苇光合作用固定HCO3-碳量为159.60 t·a~(-1)·km~(-2),水葫芦为10.80 t·a~(-1)·km~(-2),金鱼藻为9.24 t·a~(-1)·km~(-2),平均值为59.88 t·a~(-1)·km~(-2)。会仙喀斯特湿地植物的不同生活型、光合作用途径和生长微环境,是影响叶片δ~(13)C变化的主要因素。     

Polymorphisms of Glucose-Regulated Protein 78 and Risk of Colorectal Cancer: A Case-Control Study in Southwest China

Glucose-regulated protein 78 (GRP78), an endoplasmic reticulum chaperone, up-regulation serves as an efficient mechanism to promote malignant transformation of colorectal cancer (CRC) and protect CRC cells against apoptosis. Recently, the analysis of GRP78 polymorphisms has already determined that GRP78 rs391957 polymorphism could predict clinical outcome in CRC patients. Thus, we tested whether GRP78 polymorphisms are related to the risk of CRC. In this study, we detected two GRP78 polymorphisms (rs391957 (C>T) and rs430397 (G>A)) in 414 CRC cases and 502 hospital-based cancer-free healthy controls in Southwest China using a polymerase chain reaction–restriction fragment length polymorphism technique. Compared with the CC genotype, carriers of CT and TT genotypes of rs391957 polymorphism had higher risks of CRC (odds ratio (OR) = 1.39, 95% confidence interval (CI) = 1.06–1.83 for CT genotype and OR = 2.10, 95% CI = 1.06–4.14 for TT genotype, respectively). In CRC cases, the variant T allele was significantly associated with tumor invasion stage (P = 0.030), but not with status of lymph nodes metastasis (P = 0.052). Compared with the GG genotype, carriers of GA and AA genotypes of rs430397 polymorphism had higher risks of CRC (OR = 1.63, 95% CI = 1.23–2.15 for GA genotype and OR = 2.92, 95% CI = 1.23–6.94 for AA genotype, respectively). The rs430397 polymorphism was not associated with the clinicopathological characteristics of CRC. These data provide the first evidence that GRP78 rs391957 and rs430397 polymorphisms could serve as markers to predict the risk of CRC.