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排序方式: 共有641条查询结果,搜索用时 15 毫秒
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Yanyan Han Elfriede Eppinger Ingrid G. Schuster Luise U. Weigand Xiaoling Liang Elisabeth Kremmer Christian Peschel Angela M. Krackhardt 《The Journal of biological chemistry》2009,284(48):33409-33417
The formin protein formin-like 1 (FMNL1) is highly restrictedly expressed in hematopoietic lineage-derived cells and has been previously identified as a tumor-associated antigen. However, function and regulation of FMNL1 are not well defined. We have identified a novel splice variant (FMNL1γ) containing an intron retention at the C terminus affecting the diaphanous autoinhibitory domain (DAD). FMNL1γ is specifically located at the cell membrane and cortex in diverse cell lines. Similar localization of FMNL1 was observed for a mutant lacking the DAD domain (FMNL1ΔDAD), indicating that deregulation of autoinhibition is effective in FMNL1γ. Expression of both FMNL1γ and FMNL1ΔDAD induces polarized nonapoptotic blebbing that is dependent on N-terminal myristoylation of FMNL1 but independent of Src and ROCK activity. Thus, our results describe N-myristoylation as a regulative mechanism of FMNL1 responsible for membrane trafficking potentially involved in a diversity of polarized processes of hematopoietic lineage-derived cells. 相似文献
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Dieter Ehlers Ina Sakowski Werher Mohrig 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1989,159(4):481-489
Summary The high affinity of granulocytes of guinea pig and man to glass surfaces is modified by serum. Native serum contains both an adherence-promoting activity, which is related to complement, and components which reduce the adhesiveness of granulocytes. These components are stable at 56°C for 30 min and are tightly bound to the glass surface. -Lipoproteins are candidates for this adherence reducing ability of serum. Adherence promotion by native serum is mediated by coating the glass surface with C3b/C3bi. Human granulocytes from the peripheral blood adhered to glass surfaces coated by native human or guinea pig serum with C3b/C3bi to almost the same extent as in the presence of native serum, but on guinea pig granulocytes elicited in the peritoneal cavity, a cell surface metalloproteinase degraded the C3b/C3bi, thus reducing the adhesiveness of these cells. This proteinase was inhibited by MgEDTA, DTT, and 1,10-phenanthroline, whereby the high adhesiveness of granulocytes was restored to C3b/C3bi-coated glass.Abbreviations
BA
benzamidine hydrochloride
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BTS
Bacillus thuringiensis subtoxicus
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DTT
dithiothreitol
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EAC
-amino-caproic acid
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gp
guinea pig
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LDL
low density lipoproteins
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SEM
scanning electron microscopy 相似文献
5.
Mouse soleus muscle Na-K pump activity: direct correlation with in vitro and in vivo oxygen consumption 总被引:1,自引:0,他引:1
To evaluate the contribution of active sodium-potassium transport to energy utilization, measurements of sodium-potassium pump activity in isolated soleus muscles of normal mice were carried out and related to measurements of oxygen consumption by the tissue. A highly significant positive correlation (r = 0.89, p less than 0.001) was found between these two parameters. In addition, both the in vitro oxygen consumption and Na-K pump activity of the muscles were found to correlate with whole body oxygen utilization by the experimental animals. The results support a role for the sodium-potassium pump in the determination of energy turnover. 相似文献
6.
Shlomo Ben-Efraim Luise Komlos Jaffa Notmann Jacob Hart Isaac Halbrecht 《Cancer immunology, immunotherapy : CII》1985,19(1):53-56
Summary In vitro treatment with 2 g/2×106 cells melphalan (l-PAM: l-phenylalanine mustard) significantly decreased the total number of T lymphocytes from peripheral blood (PBL) of healthy human donors and of the OKT4 population (precursor suppressor/helper/inducer) T cells as defined by monoclonal antibodies OKT3 and OKT4, respectively. No changes in the OKT
8
+
lymphocyte population (cytotoxic/mature suppressor cells) were observed following the same treatment. Preincubation of PBL with l-PAM at concentrations that do not affect the rate of DNA synthesis in PHA-stimulated lymphocytes inhibited the generation of T suppressor lymphocytes by ConA, as shown by their effect on PHA stimulation. Treatment of allogeneic PBL with l-PAM had no effect on mature suppressor T cells already induced by Con A, as shown by incubation of PBL with l-PAM after incubation with ConA. 相似文献
7.
Hans -Ortwin Nalbach Jochen Zeil Luise Forzin 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1989,165(5):643-649
1. | We recorded compensatory eye stalk movements in response to pitch and roll stimulation of the visual, statocyst, and leg-proprioceptive systems in different species of crabs (Carcinus maenas, Heloecius cordiformis, Pachygrapsus marmoratus) (Fig. 2). |
2. | The relative contribution of visual, statocyst and leg-proprioceptive inputs to eye stabilization in space varies greatly among different species (Fig. 3). |
3. | We suggest that for stabilizing the eyes in space, the contribution of various sensory inputs in different species of crabs correspond to the availability of cues in their habitat. Semiterrestrial crabs living in a habitat with well defined and predictable visual geometry stabilize their eye stalks mainly by visual cues. Crabs living on solid substrate make strong use of leg proprioceptive input. Swimming crabs, and other predominantly aquatic crabs, rely mainly on their statocysts. |
8.
Chathurika Henpita Rajesh Vyas Chastity L. Healy Tra L. Kieu Aditi U. Gurkar Matthew J. Yousefzadeh Yuxiang Cui Aiping Lu Luise A. Angelini Ryan D. O'Kelly Sara J. McGowan Sanjay Chandrasekhar Rebecca R. Vanderpool Danielle Hennessy-Wack Mark A. Ross Timothy N. Bachman Charles McTiernan Smitha P. S. Pillai Warren Ladiges Mitra Lavasani Johnny Huard Donna Beer-Stolz Claudette M. St. Croix Simon C. Watkins Paul D. Robbins Ana L. Mora Eric E. Kelley Yinsheng Wang Timothy D. O'Connell Laura J. Niedernhofer 《Aging cell》2023,22(4):e13782
Cardiomyopathy is a progressive disease of the myocardium leading to impaired contractility. Genotoxic cancer therapies are known to be potent drivers of cardiomyopathy, whereas causes of spontaneous disease remain unclear. To test the hypothesis that endogenous genotoxic stress contributes to cardiomyopathy, we deleted the DNA repair gene Ercc1 specifically in striated muscle using a floxed allele of Ercc1 and mice expressing Cre under control of the muscle-specific creatinine kinase (Ckmm) promoter or depleted systemically (Ercc1−/D mice). Ckmm-Cre+/−;Ercc1−/fl mice expired suddenly of heart disease by 7 months of age. As young adults, the hearts of Ckmm-Cre+/−;Ercc1−/fl mice were structurally and functionally normal, but by 6-months-of-age, there was significant ventricular dilation, wall thinning, interstitial fibrosis, and systolic dysfunction indicative of dilated cardiomyopathy. Cardiac tissue from the tissue-specific or systemic model showed increased apoptosis and cardiac myocytes from Ckmm-Cre+/-;Ercc1−/fl mice were hypersensitive to genotoxins, resulting in apoptosis. p53 levels and target gene expression, including several antioxidants, were increased in cardiac tissue from Ckmm-Cre+/−;Ercc1−/fl and Ercc1−/D mice. Despite this, cardiac tissue from older mutant mice showed evidence of increased oxidative stress. Genetic or pharmacologic inhibition of p53 attenuated apoptosis and improved disease markers. Similarly, overexpression of mitochondrial-targeted catalase improved disease markers. Together, these data support the conclusion that DNA damage produced endogenously can drive cardiac disease and does so mechanistically via chronic activation of p53 and increased oxidative stress, driving cardiac myocyte apoptosis, dilated cardiomyopathy, and sudden death. 相似文献
9.
Jens M. Olesen Yoko L. Dupont Bodil K. Ehlers Alfredo Valido Dennis M. Hansen 《Nordic Journal of Botany》2003,23(5):537-539
Jasminum odoratissimum is a Madeira and Canary Islands endemic showing classic heterostyly, i.e. with long-styled flowers with anthers at a low level in the corolla tube and short-styled flowers with anthers at a high level in the corolla tube. Short-styled flowers have large pollen, whereas long-styled flowers have small pollen. The two types are present in equal frequencies in the population. 相似文献
10.
The basic organization of the Plathelminthes 总被引:2,自引:2,他引:0
Ulrich Ehlers 《Hydrobiologia》1995,305(1-3):21-26
The basic organization of the Plathelminthes is summarized. Special attention is given to epidermal structures, musculature, extracellular matrices, nervous system and sensory structures, digestive system, totipotent stem cells, protonephridia, reproductive system and life cycle. The latest common ancestor of the Plathelminthes lacked any parenchymal cells and tissues; Plathelminthes do not represent Parenchymia. Discussions concerning the relationships of the Plathelminthes with other Metazoa must be based on the characteristics of the plathelminth stem species. 相似文献