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ZHANG KezhongJIANG PeihongLU DaruHUANG WeidaCHEN LiXUE Jerry L. HsuehQIU Xinfang 《中国科学:生命科学英文版》1998,41(4):406-412
Mammary gland specific expression vectors for human clotting factor IX (hFIX) and LacZ reporter gene driven by bovine β-casein
gene were constructed. Vectors were packaged by stearylamine (SA) liposome and were transferred to lactating mice via tail
vein. Both hFIX and Lac2 gene could be expressed in the mammary gland of the treated mice. The highest production of hFIX
protein was 80.28 ng per mL milk, and more than 85% of hFIX protein appeared to be γ-carboxylation and biologically active.
The results suggested that the 2.0 kb sequence of β-casein gene including promoter, exon 1 was effective to drive hFIX gene
expression in mammary gland and intron 1 of β-casein gene had an effect on the tissue specific expression. The expression
level in mouse milk injected with hFIX minigene vector containing hFIX endogenous intron 1 was increased by above 3 times
of that injected with hFIX cDNA vector.
Project supported by the State High Technology Development Program and Shanghai Science and Technology Commission. 相似文献
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