Synaptic polarity and sign-balance prediction using gene expression data in the Caenorhabditis elegans chemical synapse neuronal connectome network

Graph theoretical analyses of nervous systems usually omit the aspect of connection polarity, due to data insufficiency. The chemical synapse network of Caenorhabditis elegans is a well-reconstructed directed network, but the signs of its connections are yet to be elucidated. Here, we present the gene expression-based sign prediction of the ionotropic chemical synapse connectome of C. elegans (3,638 connections and 20,589 synapses total), incorporating available presynaptic neurotransmitter and postsynaptic receptor gene expression data for three major neurotransmitter systems. We made predictions for more than two-thirds of these chemical synapses and observed an excitatory-inhibitory (E:I) ratio close to 4:1 which was found similar to that observed in many real-world networks. Our open source tool (http://EleganSign.linkgroup.hu) is simple but efficient in predicting polarities by integrating neuronal connectome and gene expression data.     

A simple integrative electrophysiological model of bursting GnRH neurons

In this paper a modular model of the GnRH neuron is presented. For the aim of simplicity, the currents corresponding to fast time scales and action potential generation are described by an impulsive system, while the slower currents and calcium dynamics are described by usual ordinary differential equations (ODEs). The model is able to reproduce the depolarizing afterpotentials, afterhyperpolarization, periodic bursting behavior and the corresponding calcium transients observed in the case of GnRH neurons.     

Changes in plasma testosterone, thyroxine and triiodothyronine in relation to sperm production and remex moult in domestic ganders

Changes in plasma testosterone (T), thyroxine (T4), triiodothyronine (T3), semen output and remex moult were studied in domestic ganders. A bimodal pattern in both plasma T and sperm concentration was observed during the annual cycle. Ganders started to produce semen at the end of January; maximum semen volume (0.32 +/- 0.04 ml) and sperm concentration (148 +/- 38 x 10(3)/mm3) were reached in March and a marked decrease was observed after mid-April, when the moult of the remiges began. Plasma T3 levels peaked in February (9.7 +/- 0.6 nmol.l-1) and this peak coincided with maximum T concentrations (9.8-10.4 nmol.l-1). Elevated levels of T4 were found from late February until mid-April (31.0-33.6 nmol.l-1). Plasma T concentration was low at all stages of remex moult and regrowth. Decreased T4 levels were found in ganders during remex regrowth from the "brush" to half of the full primary growth stage. Higher plasma T4 levels were found before and after this stage of the moult. A reverse pattern was observed for T3 concentrations.     

The origin and ultrastructural characteristics of corticotropin-releasing factor (CRF)-immunoreactive nerve fibers in the posterior pituitary of the rat

Summary A fine network of corticotropin-releasing factor (CRF)-immunopositive fibers was found in the posterior lobe of the pituitary of the rat. The intermediate and distal lobes were free of CRF-immunoreactivity. Varicose, terminal-like axons were frequently observed around capillary vessels. Surgical isolation of the paraventricular nuclei resulted in a complete disappearance of CRF-immunoreactive fibers from the posterior lobe. CRF-immunopositive fibers show the general characteristics of peptidergic axons. These ultrastructural observations support the idea that CRF is secreted into capillary vessels.     

Structure and expression of cloned murine IFN-alpha genes

The mouse has an interferon-alpha (MuIFN-alpha) gene family containing at least four, and likely more than ten members. A segment of mouse chromosomal DNA and cDNAs encoding murine alpha IFNs have been cloned, and the sequence of two MuIFN-alpha DNAs determined. No intron was found in the chromosomal gene. The two coding sequences produced biologically active IFN when expressed in monkey cells under the control of an SV40 promoter, and in E.coli under the control of the ampicillinase promoter. MuIFN-alpha 1 had no detectable activity on human cells, while MuIFN-alpha 2 was 20% as active on human as on mouse cells.     

Inhibition of protein synthesis directed by added viral and cellular messenger RNAs in extracts of interferon-treated Ehrlich ascites tumor cells. Location and dominance of the inhibitor(s)

Protein synthesis directed by exogenous (viral or cellular) messengers is impaired, but endogenous protein synthesis is not affected in an extract of interferon-treated Ehrlich ascites tumor cells (INT-extract). Protein synthesis directed by exogenous messengers is also impaired in a mixture of an INT-extract with an extract from control cells. This reveals that the impairment is due to one or more inhibitors in the INT-extract. The nondialyzability of the inhibitor(s) is probably an indication of large molecular size. In a not incubated INT-extract much of the inhibitory activity is in the high speed sediment fraction i.e., is presumably bound directly or indirectly to ribosomes. During incubation of the extract most of the inhibitory activity is released into the high speed supernatant fraction. The dose-response curve shows that in our conditions the translation of cellular messengers (from mouse L cells) is as sensitive to impairment by the inhibitor(s) as that of viral messengers (from reovirus or from encephalomyocarditis virus).