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1.
Characterization of human autoantibodies specific for lamin A 总被引:2,自引:0,他引:2
J C Courvalin N Chaudhary F Danon J C Brouet K Lassoued 《Biology of the cell / under the auspices of the European Cell Biology Organization》1990,69(2):93-97
We have characterized human autoimmune polyclonal antibodies reactive with lamin A, a 74 kDa peripheral protein of the nuclear envelope. Unlike other known antibodies to lamin A, the antibodies described here do not crossreact with the structurally related lamin C. These antibodies feature only chi light chains suggesting that their specificity is restricted to a limited number of epitopes. Based on the known amino acid sequence of human lamins A and C, the epitope(s) are most likely located in the 80 amino acid carboxyl tail of mature lamin A. 相似文献
2.
Annabi Berrahal A Nehdi A Hajjaji N Gharbi N El-Fazâa S 《Comptes rendus biologies》2007,330(8):581-588
Lead toxicity is closely related to its accumulation in several tissues and its interference with bioelements, whose role is critical for several biological processes. Recently, oxidative stress has been proposed as a possible mechanism involved in lead toxicity. This study was carried out to investigate the effect of dose-dependent lead exposure on haematological and oxidative stress parameters. Adult male 'Wistar' rats (150-200 g) were divided into three groups: group [Pb 5] and group [Pb 15] received respectively 5 mg Pb(2+) (n=16) and 15 mg Pb(2+)/kg b.w. (n=16) as lead acetate solution i.p. for a period of seven days. Group [T] (n= 16) served as control and received 15 mg Na(+)/kg b.w. as sodium acetate solution i.p. for the same period. All animals were sacrificed 24 h after the last injection. Blood superoxide dismutase (SOD) and blood glutathione peroxidase (GPx) activities and plasma bilirubin level were measured. Liver was quickly excised for the estimation of alteration in lipid peroxidation indices (MDA). Lead exposure induces, in both treated groups, a marked decline in haematocrit and haemoglobin levels (p<0.01) when compared to control. The results show also a significant decrease (p<0.01) in SOD activity, but only in group [Pb 15]. SOD activity did not decrease in group [Pb 5] in comparison with control (p>0.05). However, lead exposure caused a light increase in GPx activity in group [Pb 15], which remains non-significant (p>0.05) compared to control. Group [Pb 5] did not record significant changes in the activity of GPx. Lead exposure for a period of seven days resulted in a significant (p<0.05) increase in bilirubin level in group [Pb 15] compared to control. The bilirubin level from rats of group [Pb 5] did not reach a statistical significance. Changes in liver MDA content in lead-exposed rats from [Pb 5] and [Pb 15] groups did not reach a statistical (p<0.05) significance. It is concluded that lead induces oxidative stress in a dose-dependent manner. No dose-dependent response was observed in blood GPx activity and liver MDA content. These results could be due to the short duration of the treatment. 相似文献
3.
Haemochromatosis is predominantly associated with the HFE p.C282Y homozygous genotype, which is present in approximately 1 in 200 people of Northern European origin. However, not
all p.C282Y homozygotes develop clinical features of haemochromatosis, and not all p.C282Y homozygotes even present abnormal
iron parameters justifying venesection therapy. This situation was not apparent from initial genotype/phenotype correlation
studies as there was a selection bias of patients. Only those patients with a significant iron burden were included in these
early studies. It is now largely accepted that the p.C282Y/p.C282Y genotype is necessary for the development of HFE haemochromatosis. However, this genotype provides few clues as to why certain symptoms are associated with the disease. Expression
of iron overload in people with this genotype depends on the complex interplay of environmental factors and modifier genes.
In this review, we restrict our discussion to work done in humans giving examples of animal models where this has helped clarify
our understanding. We discuss penetrance, explaining that this concept normally does not apply to autosomal recessive disorders,
and discuss the usefulness of different biochemical markers in ascertaining iron burden. Hepcidin, a peptide synthesized primarily
by the liver, has been identified as the central regulator in iron homeostasis. Consequently, understanding its regulation
is the key. We conclude that the main goal now is to identify important modifiers that have a significant and unambiguous
effect on iron storage. 相似文献
4.
I Ben Charfeddine FG Riepe E Clauser A Ayedi S Makni MT Sfar H Sboui N Kahloul H Ben Hamouda S Chouchane S Trimech N Zouari S M'rabet F Amri A Saad PM Holterhus M Gribaa 《Gene》2012,507(1):20-26
Congenital adrenal hyperplasia (CAH) is an autosomal recessive disease of steroid biosynthesis in humans. More than 90% of all CAH cases are caused by mutations of the 21-hydroxylase gene (CYP21A2), and approximately 75% of the defective CYP21A2 genes are generated through an intergenic recombination with the neighboring CYP21A1P pseudogene. In this study, the CYP21A2 gene was genotyped in 50 patients in Tunisia with the clinical diagnosis of 21-hydroxylase deficiency. CYP21A2 mutations were identified in 87% of the alleles. The most common point mutation in our population was the pseudogene specific variant p.Q318X (26%). Three novel single nucleotide polymorphism (SNP) loci were identified in the CYP21A2 gene which seems to be specific for the Tunisian population. The overall concordance between genotype and phenotype was 98%. With this study the molecular basis of CAH has been characterized, providing useful results for clinicians in terms of prediction of disease severity, genetic and prenatal counseling. 相似文献
5.
Olfa Abida Riadh Ben Mansour Bochra Gargouri Mourad Ben Ayed Abderrahmen Masmoudi Hamida Turki Hatem Masmoudi Saloua Lassoued 《Biological trace element research》2012,150(1-3):74-80
Pemphigus is an autoimmune disorder resulting from the interaction between autoantibodies and desmoglein. Oxidative stress seems to be responsible for the onset/aggravation of many human diseases. Actually, it is considered as one of the several factors for the etiopathogenesis of pemphigus. The present study aims to evaluate the oxidative state in the sera of pemphigus vulgaris and pemphigus foliaceus patients by assessing lipid peroxidation, proteins oxidation, and antioxidant enzyme activity. This study included 36 pemphigus vulgaris and 42 pemphigus foliaceus patients as well as a group of controls consisting of 78 healthy volunteers. Malondialdehyde levels (p?<?0.001) and catalase activity (p?<?0.001) are higher in both groups of patients than in the control group. The two groups of patients showed a nonsignificant decrease in the thiol groups compared with the healthy one. A nonsignificant difference was shown between pemphigus vulgaris and pemphigus foliaceus patients, except for the catalase which shows an increase in the pemphigus vulgaris group. We have also found significant correlations between serum oxidative stress marker levels and serum anti-desmoglein antibody levels in the two pemphigus groups. These findings underline the implication of oxidative stress in the physiopathology of pemphigus by the increase in the autoantibodies?? reactivity. 相似文献
6.
Rouis Z Elaissi A Abid NB Lassoued MA Cioni PL Flamini G Aouni M 《Chemistry & biodiversity》2012,9(4):806-816
The chemical composition of the essential oils of five populations of Hypericum triquetrifolium Turra from Tunisia and their intraspecific variability were analyzed in detail by GC/MS. One hundred seventy-four compounds were identified, representing averages of 87.9 to 98.7% of the oil composition. The components are represented here by homologous series of monoterpene hydrocarbons, oxygenated monoterpenes, sesquiterpenes hydrocarbons, oxygenated sesquiterpenes, non-terpenic hydrocarbons, and others. Sesquiterpene hydrocarbons were the most abundant chemical compounds. Multivariate chemometric techniques, such as cluster analysis (CA) and principal-component analysis (PCA), were used to characterize the samples according to the geographical origin. By statistical analysis, the analyzed populations were classified into four chemotype groups. 相似文献
7.
8.
Lassoued S Ben Ameur R Ayadi W Gargouri B Ben Mansour R Attia H 《Molecular and cellular biochemistry》2008,313(1-2):179-186
The study investigates the direct effect of Epstein-Barr virus infection on the oxidative profile of in vitro cultivated human cells. For this purpose, a panel of human EBV target cells presenting heterogeneity in their cellular and culture types (epithelial cells or lymphocytes; primary culture or continuous cell culture) was selected. These cells are purified human B lymphocytes, DG75, 293, and HepG2 cell lines. The oxidative stress was evaluated during the early stages of infection (2, 12, and 24 h) by measuring malondialdehyde, the end product of the lipid peroxidation, as well as the activities of two antioxidant enzymes: catalase and superoxide dismutase. The obtained results were compared with those of the untreated cells and the K562 cell line which has no interaction with EBV. The incubation of the different target cells with EBV induced an oxidative stress in the purified B lymphocytes, DG75, and 293, but not in HepG2 and K562. This oxidative stress was highlighted by an increase in MDA level (P < 0.05), which began 2 h after the addition of the virus and persisted after 12 and 24 h. Simultaneously, a decrease in catalase and superoxide dismutase activities was observed (P < 0.05), suggesting an alteration of the molecular mechanisms promoting cellular resistance to reactive oxygen species (ROS). The efficiency of EBV infection, assessed by viral DNA PCR amplification, was confirmed in 293 and DG75 but not in HepG2, which was in total concordance with their oxidative profiles. In conclusion, the EBV infection of B and epithelial cells leads to the establishment of an oxidative stress which can play a key role during the viral transformation. 相似文献
9.
Phytoremediation of domestic wastewater using a hybrid constructed wetland in mountainous rural area
Saloua Elfanssi Naaila Ouazzani Lahbib Latrach Abdessamed Hejjaj 《International journal of phytoremediation》2018,20(1):75-87
The purpose of this study is to evaluate the efficiency of hybrid constructed wetlands (HCWs) in a rural mountainous area. The experiment was set up in small rural community named Tidili within the region of Marrakech, Morocco. The wastewater treatment plant was composed of three vertical flow constructed wetlands (VFCWs) working in parallel, followed by two parallel horizontal-subsurface flow constructed wetlands (HFCWs), with hydraulic loading rates of 0.5 and 0.75 m3/m2.d, respectively. The two units were planted with Phragmites australis at a density of 4 plants/m2. Wastewater samples were collected at the inlet of the storage tank and at the outlet of the whole system (VFCWs, HFCWs) stages. The main removal percentages of total suspended solids (TSS), biochemical oxygen demand measured in a 5-day test (BOD5), chemical oxygen demand (COD), total nitrogen, and total phosphorus were respectively 95%, 93%, 91%, 67%, and 62%. The system showed a very high capacity to remove total coliforms, fecal coliforms, and fecal streptococci (4.46, 4.31, and 4.10 Log units, respectively). Artificial neural networks (ANNs) were used to model the quality parameters (TSS, BOD5, COD) and total coliforms and fecal streptococci. Based on the obtained results, the ANN model could be considered as an efficient tool to predict the studied phytoremediation performances using HCWs. 相似文献
10.
Microparticle miRNAs as Biomarkers of Vascular Function and Inflammation Response to Aerobic Exercise in Obesity? 下载免费PDF全文
Saloua Dimassi Esma Karkeni Pascal Laurant Zouhair Tabka Jean‐François Landrier Catherine Riva 《Obesity (Silver Spring, Md.)》2018,26(10):1584-1593