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排序方式: 共有104条查询结果,搜索用时 15 毫秒
1.
Aino Laatikainen Leslie Schultz-Suhonen Rauno Mäntyjärvi 《Cancer immunology, immunotherapy : CII》1992,35(3):205-210
Summary The effect of interferon (IFN) on the immunogenicity and immunosensitivity of mouse cell lines transformed by bovine papillomavirus type 1 (BPV1) DNA was examined in a syngeneic mouse model. The overnight incubation of BPV1-transformed cell lines with 100 IU/ml IFN did not affect their ability to induce the generation of cytotoxic effector cells but it clearly increased their sensitivity to lysis by interleukin-2-induced lymphokine-activated killer (LAK) cells and by nonspecific LAK-type effector cells induced by BPV-1-transformed cell lines. The treatment of two allogeneic lymphoid tumour cell lines, P815X2 and YAC-1, with IFN either decreased or had no effect on their sensitivity to LAK-cell-mediated lysis. 相似文献
2.
Reino Heikkil Tore Godal Aase Henriksen Jens-Gustav Iversen 《Experimental cell research》1981,136(2):447-454
F(ab′)2 anti-μ, as well as anti-δ and anti-γ heavy chains have been found to induce strong increases in the influx of 86Rb+ (a potassium analogue) in cell suspensions from a proportion of human lymphomas staining monoclonally for B cells. The specificity of the response corresponded to the surface immunoglobulin (sIg) isotype present on lymphoma cells. Even when carried out under closely analogous conditions, no relationship between capping and 86Rb+ influx was observed. On the other hand, an association between 86Rb+ influx and mitogenic response to anti-immunoglobulin (anti-Ig) was observed with concordant results in 19 out of 21 comparisons made. These findings suggest that 86Rb+ influx, which appears to be associated with mitogenic signals, and capping may arise as independent phenomena even when elicited through the same membrane receptor (sIg). 相似文献
3.
Isolation and Identification of Aspergillus fumigatus Mycotoxins on Growth Medium and Some Building Materials 总被引:1,自引:0,他引:1 下载免费PDF全文
Susanna M. Nieminen Riikka Krki Seppo Auriola Mika Toivola Hartmut Laatsch Reino Laatikainen Anne Hyvrinen Atte von Wright 《Applied microbiology》2002,68(10):4871-4875
Genotoxic and cytotoxic compounds were isolated and purified from the culture medium of an indoor air mold, Aspergillus fumigatus. One of these compounds was identified as gliotoxin, a known fungal secondary metabolite. Growth of A. fumigatus and gliotoxin production on some building materials were also studied. Strong growth of the mold and the presence of gliotoxin were detected on spruce wood, gypsum board, and chipboard under saturation conditions. 相似文献
4.
5.
José Luis Reino Raul F. Guerrero Rosario Hernández-Galán Isidro G. Collado 《Phytochemistry Reviews》2008,7(1):89-123
Trichoderma species are free-living fungi that are highly interactive in root, soil and foliar environments and have been used successfully
in field trials to control many crop pathogens. Structural and biological studies of the metabolites isolated from Trichoderma species are reviewed. This review, encompassing all the literature in this field up to the present and in which 269 references
are cited, also includes a detailed study of the biological activity of the metabolites, especially the role of these metabolites
in biological control mechanisms. Some aspects of the biosynthesis of these metabolites and related compounds are likewise
discussed. 相似文献
6.
Laatikainen LE Incoronato M Castellone MD Laurila JP Santoro M Laukkanen MO 《PloS one》2011,6(8):e24456
Background
Extracellular superoxide dismutase (SOD3), which dismutates superoxide anion to hydrogen peroxide, has been shown to reduce the free radical stress derived apoptosis in tissue injuries. Since both superoxide anion and hydrogen peroxide have a marked impact on signal transduction pathways and could potentially explain a number of apoptosis and survival -related phenomena in different pathological conditions, we clarified the impact of SOD3 on Akt and Erk1/2 cell survival pathways in rat hind limb injury model.Methodology and Principal Findings
Based on our data, the hind limb ischemic rats treated with virally delivered sod3 have milder injury and less apoptosis than control animals that could be due to parallel activation of pro-proliferative and anti-apoptotic Erk1/2 and Akt pathways. The common downstream factor of both signaling pathways, the apoptosis related forkhead box protein O3a (FoxO3a), was phosphorylated and translocated to the cytoplasm in sod3 treated tissues and cell line. Additionally, we obtained increased mRNA production of elk-1, ets-1, and microRNA 21 (miR-21), whereas synthesis of bim mRNA was decreased in sod3 overexpressing tissues. We further showed that overexpression of sod3 modulated redox related gene expression by downregulating nox2 and inos when compared to injured control animals.Conclusions and Significance
The study shows the complexity of SOD3-derived effects on tissue injury recovery that are not limited to the reduction of superoxide anion caused cellular stress but highlights the impact of SOD3 related signal transduction on tissue functions and suggests an important role for SOD3 in attenuating cell stress effects in different pathological conditions. 相似文献7.
8.
Lehtivarjo J Tuppurainen K Hassinen T Laatikainen R Peräkylä M 《Journal of biomolecular NMR》2012,52(3):257-267
While chemical shifts are invaluable for obtaining structural information from proteins, they also offer one of the rare ways
to obtain information about protein dynamics. A necessary tool in transforming chemical shifts into structural and dynamic
information is chemical shift prediction. In our previous work we developed a method for 4D prediction of protein 1H chemical shifts in which molecular motions, the 4th dimension, were modeled using molecular dynamics (MD) simulations. Although
the approach clearly improved the prediction, the X-ray structures and single NMR conformers used in the model cannot be considered
fully realistic models of protein in solution. In this work, NMR ensembles (NMRE) were used to expand the conformational space
of proteins (e.g. side chains, flexible loops, termini), followed by MD simulations for each conformer to map the local fluctuations.
Compared with the non-dynamic model, the NMRE+MD model gave 6–17% lower root-mean-square (RMS) errors for different backbone
nuclei. The improved prediction indicates that NMR ensembles with MD simulations can be used to obtain a more realistic picture
of protein structures in solutions and moreover underlines the importance of short and long time-scale dynamics for the prediction.
The RMS errors of the NMRE+MD model were 0.24, 0.43, 0.98, 1.03, 1.16 and 2.39 ppm for 1Hα, 1HN, 13Cα, 13Cβ, 13CO and backbone 15N chemical shifts, respectively. The model is implemented in the prediction program 4DSPOT, available at . 相似文献
9.
Heikki Kallio Reino R. Linko Tapani Pyysalo Irmeli Puntari 《Analytical biochemistry》1978,90(1):359-364
The major keto acids in arctic bramble,Rubus arcticus L. were investigated. The acids were isolated with anionic and cationic ion-exchange resins, converted to 2,4-dinitrophenylhydrazones, and purified with an Al2O3 column. The derivatives were separated on a silica gel G thin-layer plate and esterified with methanol-HCl and the methyl esters of the keto acid 2,4-dinitrophenylhydrazones formed were analyzed on an OV-1-glass capillary gas-liquid chromatography column and with mass spectrometry. 2-Oxoglutaric, pyruvic, oxaloacetic, and glyoxylic acids were identified. The mass spectra of the derivatives are presented. 相似文献
10.