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A device counting fixed amount of parcels of radiant energyefficient in photosynthesis (wavelength region 400 to 720 and recording their total as a digital reading on a counteris described. Radiant energy is transformed by means of a phototubeto electrical current which becomes divided into individualparcels by cycles of charging .and discharge of a condenserbetween two fixed values of potential, and the number of thesecycles is counted. From instruments of similar design published earlier the onepresented here is distinguished mainly (1) by improved linearityof response in the integrating circuit down to slow countingrates and (2) by the design of a nonselective detector (in thelimited range of wavelengths indicated) based on a vacuum emissionphototube, which may be combined with the simple integratingcircuit used. Thus totals of irradiance (throughout the rangeof intensities of solar radiation) may be obtained directlyin units of energy or in the number of quanta. A very reliable and stable receiver with energy proportionalresponse in the region of wavelength 400–720 (with possible extension up to 1,000 ) was realizedusing a vacuum emission photocell with photosensitive surfaceof the double-peak emission type (covering the range from nearUV to near IR) and precisely counterbalancing its spectral sensitivitycurve by selective absorption glass filters. The RENTSCHLER's type integrating circuit with a cold cathodedischarge tube was improved by increasing the maximum countingrate on one side (damping germanium diodes across the coilsof relay and counter) and by minimization of leakage in parallelto spare condenser (the contact which shortcircuits the condensersimultaneously with ignition of the discharge tube). 1 Dedicated to Prof. H. TAMIYA on his 60th birthday. (Received November 24, 1962; )  相似文献   
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Hyolitha constitute an extinct group of class rank assigned to Molhsca. Two orders, Ortho-thecida and Hyolithida, are well established and most knowledge of morphologic detail is derived from the latter. Both orders had an operculum not hinged to the conch. Hyolithida also had paired, curved, whiskerlike appendages, requiring complex musculature to move them and the operculum. The Hyolithida were probably deposit feeders living in shallow water, and accordingly were tentaculate. A reconstruction of the soft parts of this sedentary organism is given — a shallow mantle cavity on the dorsal side, anterior tentacles, a long intestine, and a reduced ventral foot. Except for the more complex musculature associated with an elaborate operculum, Orthothecida are judged to have had a similar anatomy.  相似文献   
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Phytophagous ladybird beetles of the tribe Epilachnini are a cosmopolitan, species‐rich group of significant economic importance as pests of agricultural crops. The tribe is well characterized morphologically and clearly monophyletic, but very little is known about its internal phylogenetic relationships and their genus‐level taxonomy. In order to infer the evolutionary history of Epilachnini, test its monophyly and provide a phylogeny‐based classification, we assembled a comprehensive dataset, consisting of four DNA markers (18S and 28S rRNA and 16S, COI mtDNA) and a matrix of 104 morphological characters for 153 species of Epilachnini representing all previously recognised genera, ~11% of the known species, and 14 outgroup taxa. Molecular, morphological and combined datasets were analysed using maximum likelihood, parsimony and Bayesian inference. Bayes factors and Approximately Unbiased tests (AU) were used to compare alternative phylogenetic hypotheses of unconstrained and backbone‐constrained analysis. Only 14 of the 25 included genera were recovered monophyletic, as originally defined. Afidentula Kapur, Afidenta Dieke, Afissula Kapur, Epilachna Chevrolat, Henosepilachna Li Toxotoma Weise and Mada Mulsant are shown to be poly‐ or paraphyletic; Chnootriba Chevrolat, Subafissa Bielawski, Lalokia Szawaryn & Tomaszewska and Papuaepilachna Szawaryn & Tomaszewska form monophyletic groups within larger clades of genus level. All of these genera are redefined here. The two largest genera of Epilachnini, Epilachna Chevrolat and Henosepilachna Li were represented by multiple monophyletic clades, which we described as new genera: Chazeauiana Tomaszewska & Szawaryn gen.n. ; Diekeana Tomaszewska & Szawaryn gen.n .; Fuerschia Tomaszewska & Szawaryn gen.n. and Ryszardia Tomaszewska & Szawaryn gen.n . The following new synonyms are proposed: Afissa Dieke (=Afissula Kapur); Henosepilachna Li in Li & Cook (=Subafissa Bielawski); Papuaepilachna Szawaryn & Tomaszewska (=Lalokia Szawaryn & Tomaszewska). This published work has been registered in ZooBank, http://zoobank.org/urn:lsid:zoobank.org:pub:440E7FA4‐C859‐47E0‐8335‐30D478CBA8FA .  相似文献   
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Abstract We investigated the effectiveness of short mitochondrial DNA fragments for the identification of lycid larvae. The rrnL, cox1 and nad5 mtDNA sequences from 17 specimens of immature stages of Lycidae and Lampyridae were combined with a previously published dataset of homologous fragments representing all major lineages of Lycidae and outgroups. Their relationships were analysed under parsimony criteria. We demonstrate that high‐density profiles are necessary for accurate identification of unknown samples to generic and tribal levels and that a multilocus approach is critical for obtaining reliable results. Although widely used, the cox1 mtDNA fragment showed the worst performance for identification at genus level when the query species was not present in the library. Stronger support for deeper branches came from rrnL mtDNA. The neotenic female larvae and male adult stages of Platerodrilus sp. and Macrolibnetis depressus Pic, 1938 were associated by mtDNA fragments. Based on the present identification, larvae of Dictyopterini (Dictyopterini gen. sp., Dictyoptera aurora Herbst, 1784), Sulabanus sp., Leptotrichalus sp. (Metriorrhynchini) and Macrolibnetis depressus Pic, 1938 (Platerodrilini) are described for the first time. Further species of Platycis Thomson, 1859, Plateros Bourgeois, 1979, Macrolycus Waterhouse, 1878, Cautires Waterhouse, 1879 and Lyponia Waterhouse, 1878 are identified by morphology and molecular markers. The data on larval morphology and their usefulness for classification are discussed.  相似文献   
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The species representation of public databases is growing rapidly and permits increasingly detailed phylogenetic inferences. We present a supermatrix based on all gene sequences of Coleoptera available in Genbank for two nuclear (18S and 28S rRNA) and two mitochondrial (rrnL and cox1) genes. After filtering for unique species names and the addition of ?2000 unpublished sequences for cox1 and 18S rRNA, the resulting data matrix included 8441 species‐level terminals and 6600 aligned nucleotide positions. The concatenated matrix represents the equivalent of 2.17% of the 390 000 described species of Coleoptera and includes 152 beetle families. The remaining 29 families constitute small lineages with ?250 known species in total. Taxonomic coverage remains low for several major lineages, including Buprestidae (0.16% of described species), Staphylinidae (1.03%), Tenebrionidae (0.90%) and Cerambycidae (0.58%). The current taxon sampling was strongly biased towards the Northern Hemisphere. Phylogenetic trees obtained from the supermatrix were in very good agreement with the Linnaean classification, in particular at the family level, but lower for the subfamily and lowest for the genus level. The topology supports the basal split of Derodontidae and Scirtoidea from the remaining Polyphaga, and the broad paraphyly of Cucujoidea. The data extraction pipeline and detailed tree provide a framework for placement of any new sequences, including environmental samples, into a DNA‐based classification system of Coleoptera.  相似文献   
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Fagus sylvatica L. is one of the most widespread broad‐leaved trees in the temperate forests of the northern hemisphere. In addition to two subspecies, F. sylvatica ssp. sylvatica in Europe and F. sylvatica ssp. orientalis in south‐western Asia, two further taxa were described: F. moesiaca (Maly) Czeczott in the south‐western Balkans and F. taurica Popl. in Crimea. The opinions about the number and ranks of taxa within this complex are highly controversial. To assess the degree of genetic differentiation among them, and to reveal geographical patterns of genetic diversity and their relationships to history and biogeography of beech populations, genetic variation at 12 allozyme loci was studied in 279 populations in western Eurasia. A Bayesian analysis of population structure revealed the existence of two clusters, which fairly well coincided with F. sylvatica ssp. sylvatica and F. sylvatica ssp. orientalis, whereby the populations from the south‐western Balkans and Crimea contained a mixture of these two gene pools. On the other hand, a neighbour‐joining tree based on pairwise FST failed to separate the subspecies into well‐defined distinct clades. Populations of F. sylvatica ssp. orientalis proved to be incomparably more differentiated than ssp. sylvatica (FST = 0.157 and 0.032, respectively). Asian populations also showed higher levels of allelic richness both on population and taxon levels than the European ones (the number of alleles after rarefaction was 3.40 and 4.27 in F. sylvatica ssp. sylvatica and ssp. orientalis, respectively). This indicates that the gene pool of F. sylvatica ssp. orientalis has not been depleted by reduced population sizes during the Pleistocene glaciations, as is the case of F. sylvatica ssp. sylvatica. Genetic similarities between isolated regional populations are explained by shared ancestral polymorphisms and/or range overlaps with subsequent hybridization in the past. © 2007 The Linnean Society of London, Botanical Journal of the Linnean Society, 2007, 154 , 165–174.  相似文献   
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