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Chaikeeratisak V Somboonwiwat K Wang HC Lo CF Tassanakajon A 《Molecular biology reports》2012,39(5):6367-6377
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Ponprateep S Tharntada S Somboonwiwat K Tassanakajon A 《Fish & shellfish immunology》2012,32(1):26-34
Anti-lipopolysaccharide factors (ALFs) are antimicrobial peptides previously identified in various crustaceans. Out of five isoforms identified in Penaeus monodon, ALFPm3 is the best characterized, exhibits antibacterial and antifungal activities and can protect the shrimp from viral infections. Herein, the most recent identified ALFPm, called ALFPm6, is characterized for its potential role in the shrimp’s immunity. RNA interference-mediated gene silencing was used to study the function of ALFPm6 in comparison to ALFPm3. Knockdown of ALFPm3 gene led to rapid death with a cumulative shrimp mortality of 86% within 7 days, accompanied by a 12- and 50-fold higher bacterial count after 2 days in the haemolymph and hepatopancreas, respectively, compared to the control shrimp injected with GFP dsRNA. In contrast, gene silencing of ALFPm6 alone had no effect on the shrimp mortality, but led to a significant increase in the cumulative mortality and a faster mortality rate following Vibrio harveyi and white spot syndrome virus (WSSV) infections, respectively. These results support the roles of ALFPm6 and ALFPm3 in the protection of shrimp against microbial infections. 相似文献
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Anchalee Tassanakajon Piti Amparyup Kunlaya Somboonwiwat Premruethai Supungul 《Marine biotechnology (New York, N.Y.)》2010,12(5):487-505
Penaeid shrimp aquaculture has been consistently affected worldwide by devastating diseases that cause a severe loss in production.
To fight a variety of harmful microbes in the surrounding environment, particularly at high densities (of which intensive
farming represents an extreme example), shrimps have evolved and use a diverse array of antimicrobial peptides (AMPs) as part
of an important first-line response of the host defense system. Cationic AMPs in penaeid shrimps composed of penaeidins, crustins,
and anti-lipopolysaccharide factors are comprised of multiple classes or isoforms and possess antibacterial and antifungal
activities against different strains of bacteria and fungi. Shrimp AMPs are primarily expressed in circulating hemocytes,
which is the main site of the immune response, and hemocytes expressing AMPs probably migrate to infection sites to fight
against pathogen invasion. Indeed, most AMPs are produced as early as the nauplii developmental stage to protect shrimp larvae
from infections. In this review, we discuss the sequence diversity, expression, gene structure, and antimicrobial activities
of cationic AMPs in penaeid shrimps. The information available on antimicrobial activities indicates that these shrimp AMPs
have potential therapeutic applications in the control of disease problems in aquaculture. 相似文献
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Cationic Antimicrobial Peptides in Penaeid Shrimp 总被引:1,自引:0,他引:1
Tassanakajon A Amparyup P Somboonwiwat K Supungul P 《Marine biotechnology (New York, N.Y.)》2011,13(4):639-657
Penaeid shrimp aquaculture has been consistently affected worldwide by devastating diseases that cause a severe loss in production.
To fight a variety of harmful microbes in the surrounding environment, particularly at high densities (of which intensive
farming represents an extreme example), shrimps have evolved and use a diverse array of antimicrobial peptides (AMPs) as part
of an important first-line response of the host defense system. Cationic AMPs in penaeid shrimps composed of penaeidins, crustins,
and anti-lipopolysaccharide factors are comprised of multiple classes or isoforms and possess antibacterial and antifungal
activities against different strains of bacteria and fungi. Shrimp AMPs are primarily expressed in circulating hemocytes,
which is the main site of the immune response, and hemocytes expressing AMPs probably migrate to infection sites to fight
against pathogen invasion. Indeed, most AMPs are produced as early as the nauplii developmental stage to protect shrimp larvae
from infections. In this review, we discuss the sequence diversity, expression, gene structure, and antimicrobial activities
of cationic AMPs in penaeid shrimps. The information available on antimicrobial activities indicates that these shrimp AMPs
have potential therapeutic applications in the control of disease problems in aquaculture. 相似文献
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Anchalee Tassanakajon Kunlaya Somboonwiwat Premruethai Supungul Sureerat Tang 《Fish & shellfish immunology》2013,34(4):954-967
Several immune-related molecules in penaeid shrimps have been discovered, most of these via the analysis of expressed sequence tag libraries, microarray studies and proteomic approaches. These immune molecules include antimicrobial peptides, serine proteinases and inhibitors, phenoloxidases, oxidative enzymes, clottable protein, pattern recognition proteins, lectins, Toll receptors, and other humoral factors that might participate in the innate immune system of shrimps. These molecules have mainly been found in the hemolymph and hemocytes, which are the main sites where immune reactions take place, while some are found in other immune organs/tissues, such as the lymphoid organs, gills and intestines. Although the participation of some of these immune molecules in the shrimp innate immune defense against invading pathogens has been demonstrated, the functions of many molecules remain unclear. This review summarizes the current status of our knowledge concerning the discovery and functional characterization of the immune molecules in penaeid shrimps. 相似文献
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