Genotypical characteristics of the immunological Vi-antibody maturation depending on the effect of Vi-antigen deposit in the body

The authors carried out a comparative analysis of the changes in the antibody synthesis and their immunological maturation in the immunization with various doses of the Vi-antigen of linear rats differing by the capacity to deposit this antigen. August rats with a high capacity to deposit the Vi-antigen (in comparison with Wistar rats in which this capacity was less) were characterized by an increased level of highly avid antibodies. The interlinear differences were found in the character of the changes in the antibody synthesis depending on the immunizing dose of the antigen. Genotypical peculiarities of the immunological maturation of the antibodies were demonstrated. The authors suppose that the described phenomenon was genetically determined and referred to the factors determining the individual immunological reactivity. In the choice of the optimal immunizing doses of the antigens the authors recommend complex determination of the antibody level and the degree of their avidity.     

Electrophysiological analysis of corticofugal influences on preoptic neurons

Evoked potential (EPs) and responses of the medial (MPO) and lateral (LPO) preoptic region (RPO) and adjacent structures of the hypothalamus to stimulation of the prefrontal (area 8) and cingulate (area 24) cortex, piriform lobe (periamygdaloid cortex), and hippocampus (area CA3) were investigated in acute experiments on cats under ketamine anesthesia. The most pronounced EPs were observed in the RPO after stimulating the piriform and cingulate cortex. A close relation was found between neuronal responses and EP components. The majority of neurons responding to stimulation of various cortical structures were localized in the LPO, where primarily excitatory responses dominate. The MPO contained somewhat fewer neurons responding to cortical stimulation, and the dominant response here was primarily inhibitory. The ratio of inhibitory and excitatory responses in the LPO was 0.6:1 and in the MPO 5.8:1. Primarily in-inhibitory responses dominated also in the LPO zone adjacent to the bed nucleus stria terminalis (BST) and primarily excitatory in the region surrounding the supraoptic nucleus (SO) (respective ratios 4.9:1 and 0.7:1). The RPO is a broad convergence zone, where 3/4 of the neurons responded to stimuli of two and more cortical regions.A. M. Gorky Medical Institute, Ukrainian Minstry of Health, Donetsk. Translated from Neirofiziologiya, Vol. 23, No. 6, pp. 709–719, November–December, 1991.     

Responses of neurons of the frontal area of the cortex of the rabbit to electric stimulation of certain limbic-mesencephalic structures after administration of substance P

In rabbits tested on behavioural reactions by electrical stimulation of certain limbic-midbrain structures, intravenous injection of substance P (30 mcg/kg) led after 10 min of silent period to a decrease of spontaneous neuronal activity in the frontal cortex. Convergence of excitations arising from the lateral hypothalamus, the dorsal hippocampus and the midbrain reticular formation was also found to decrease after the substance P injection. Analysis of neuronal responses allowed to establish that substance P markedly changed the ascending excitations of the lateral hypothalamus and was less effective for the influences from the midbrain reticular formation.     

Organization of neurons forming descending corticohypothalamic pathways

Retrograde horseradish peroxidase axonal transport techniques were used for quantifying the spatial organization of cortical neurons constituting descending fiber systems running to hypothalamic structures. This revealed the diversity of corticohypothalamic connections: the prefrontal cortex exerts a more marked effect on the hypothalamus than other cortical regions of the limbic system. Corticohypothalamic cells form neuronal groups at different frontal levels of the prefrontal cortex and cingulate gyrus; shape and orientation of cell groups are determined by the topography of predominant axonal systems travelling to the hypothalamus. Various forms of cell combinations occur within neuronal populations. Neuronal arrays may underlie the organizational principle of direct corticohypothalamic pathways.A. M. Gor'kii Medical Institute, Ministry of Public Health of the Ukrainian SSR, Donetsk. Translated from Neirofiziologiya, Vol. 22, No. 4, pp. 435–441, June–July, 1990.     

Firing activity of preoptic and supraoptic neurons upon mild alterations in osmo- and glucohomeostasis

Responses of neurons of the lateral (LPO) and medial (MPO) subdivisions of the preoptic region (RPO) and of the supraoptic nucleus (SO) of the hypothalamus at infusions of up to 400 µl of a hypotonic (0.2%) or a hypertonic (0.3%) NaCl solution or an isotonic (5.5%) glucose solution into the homolateral internal carotid artery were studied in acute experiments on ketamine-anesthetized cats. Changes in the firing frequency were exhibited by 69% of the examinedRPO andSO neurons, the response being of four different types: a monophasic increase (1st-type) or a monophasic decrease (2nd-type) in the activity; biphasic responses where an initial frequency rise was followed by inhibition (3rd-type) and vice versa (4th-type). Of all the neuronal responses to all stimulations, 50% (121/245) were of the 1st type; 11% (26/245), of the 2nd type; 5% (14/245), of the 3rd type; and 3% (7/245), of the 4th type. No neurons with 1st-type responses to infusion of the hypotonic NaCl solution were found in the medial sections of theMPO, and of the hypertonic solution, in the lateral sections of theLPO. Neurons with 2nd-type responses to infusion of hypo- and hypertonic NaCl solutions were almost solely detected in theSO, whereas units with such responses to glucose infusions were observed only in theRPO, being fully absent in theSO, where this stimulation gave rise only to 1st-type responses. Neurons with 3rd- and 4th-type responses to hypo- and hyperosmotic stimulations were detected predominantly in theMPO andSO, and to glucose stimulation, in theMPO andLPO.Neirofiziologiya/Neurophysiology, Vol. 25, No. 4, pp. 281–291, July–August, 1993.     

Effects of La3+ on calcium binding to erythrocyte cytoskeleton]

When the whole erythrocytes were exposed to LaCl3, A--23187, ionomycin, orthovanadate and saponin, there was Ca2+ binding only following La3+ treatment of the cells. The binding was evident at a wide range (0.1 microM--1.OmM) of La3+ concentrations. Iodoacetamide-induced (incubation for 3 hours, 37 degrees C) decrease in erythrocyte ATP levels was found to result in a 3-fold reduction in Ca2+ binding to the cytoskeleton. La(3+)-induced Ca2+ binding enhanced the incorporation of 14C-glucose and/or its metabolites into the red cell skeleton. Thus, the detected new type of Ca2+ binding to the cytoskeleton of human and rat erythrocytes is likely to be due to the cumulative process: direct binding of La3+ to the outer surface of a membrane and the metal-induced trigger of nucleotide--dependent intracellular process.     

The investigation of structure–activity relationship of polyamine-targeted synthetic compounds from different chemical groups

Amino Acids - The polyamine (PA) metabolism is involved in cell proliferation and differentiation. Increased cellular PA levels are observed in different types of cancers. Products of PA oxidation...