Polysaccharides extracted from seaweeds can function as plant biostimulants. The aim of this study was to assess the effects of Polysaccharide Enriched Extracts (PEEs) obtained from 17 Moroccan seaweeds, on tomato seed germination and plant growth. Three concentrations (0.02, 0.05 and 0.1 mg mL−1) of PEEs were applied to tomato seeds to evaluate their effect on 3 germination parameters: germination percentage (GP), germination speed (GS) and mean germination time (MGT). Metabolomic analysis by GC–MS was subsequently performed on seedlings. In the second experiment, four PEEs concentrations (0.02, 0.05, 0.1 and 0.2 mg mL−1) were used as foliar spray or as soil application to tomato plants. Their growth parameters (number of leaves, shoot length, fresh and dry weight of stem and roots) and biochemical parameters (chlorophyll a and b) were measured. Results indicated a significant increase of GP and GS associated with a significant reduction of MGT of tomato seeds treated with 0.02 mg mL−1 of PEEs obtained from Gigartina sp., Gigartina pistillata, Chondracanthus acicularis, Gelidium crinale, Schizymenia dubyi, Cystoseira. foeniculacea and Fucus spiralis. Similar results were also obtained by application of higher PEEs concentration (0.1 mg mL−1) extracted from Ulva rigida, Codium tomentosum, Codium decorticatum and Bifurcaria bifurcata. Metabolomic analysis on seedlings detected the presence of some metabolites which could possibly be involved in seed germination enhancement or inhibition. The results of the second experiment showed that the same PEEs cited above at the same concentrations enhanced plant dry weight and chlorophyll a content except Gigartina sp., C. foeniculacea and C. decorticatum. Furthermore, soil application of PEEs was more effective in improving plant growth parameters than foliar application. The study shows the potential of PEEs from Moroccan seaweed to be used as biostimulants for a sustainable agriculture.
ABSTRACTThe present study aimed to investigate whether nocturnal melatonin (MEL) ingestion has beneficial effects against exercise-induced oxidative stress and muscle damage in young athletes. Fourteen healthy-trained teenagers performed two-test sessions separated by at least, 1 week. During each session, participants completed the Running-Based Anaerobic Sprint Test (RAST) at 20:00 h. Then, they ingested a single 10-mg tablet of MEL or Placebo (PLA) in a double-blind randomized order at 22:00 h. The following morning (i.e., 07:30 h), participants performed the same test as the previous night. Blood samples were taken before and after exercise. MEL intake increased the peak power (Ppeak) (p < .01), mean power (Pmean) (p < .001) and decreased the total time (TT) (p < .001) and the fatigue index (FI) (p < .05). Furthermore, MEL ingestion attenuated the hematologic parameters before and after exercise (White Blood Cells (WBC: p < .001 and p < .001, respectively); Neutrophiles (NE: p < .001 and p < .001, respectively); Lymphocytes (LY: p < .001 and p < .001, respectively)) and the ultra-sensitive C-reactive protein (us-CRP: p < .001 and p < .001; respectively) compared to PLA. Also, MEL reduced muscle and hepatic damage enzymes before and after exercise (creatine kinase (CK: p < .001 and p < .001; respectively), lactate dehydrogenase (LDH: p < .05 and p < .01; respectively), aspartate aminotransferase (ASAT: p < .01 and p < .001; respectively)), Malondialdehyde (MDA: p < .001 and p < .001; respectively) and Homocysteine (Hcy: p < .001 and p < .001; respectively)) from placebo. Plasma lactate [La] and glucose (GL) remained unchangeable during the two conditions. In summary, acute MEL ingestion after strenuous late-evening exercise attenuated transient leucocytosis and protected against lipid peroxidation and muscle damage induced by strenuous exercise the following morning in healthy male teenage athletes. 相似文献
In marine ecosystems, fluctuations in surface-seawater carbon dioxide (CO2), significantly influence the whole metabolism of marine algae, especially during the early stages of macroalgal development. In this study, the response of the green alga Ulva fasciata for elevating ocean acidification was investigated using four levels of pCO2 ~ 280, 550, 750 and 1050 µatm. Maximum growth rate (6.6% day−1), protein (32.43 %DW) and pigment (2.9 mg/g) accumulation were observed at pCO2-550 with an increase of ~2-fold compared to control. On the other hand, lipid and carbohydrate contents recorded their maximum production (4.23 and 46.96 %DW, respectively) at pCO2-750 while control showed 3.70 and 42.37 %DW, respectively. SDS-PAGE showed the presence of unique bands in response to pCO2, especially at 550 µatm. Dominant associated bacteria was shifted from Halomonas hydrothermalis of control to Vibrio toranzoniae at pCO2-1050. These findings suggest that ocean acidification at 550 µatm might impose noticeable effects on growth, protein, pigments, and protein profile of U. fasciata, which could be a good source for fish farming. While, pCO2-750 was recommended for energetic purpose, due to its high lipid and carbohydrate contents. 相似文献
Effect of different dietary squilla chitosan (Csq) concentrations: 0 (control), 0.5, 1 and 2 g 100 g–1 diets were studied for weaned sea bass (Dicentrarchus labrax) post larvae. Post larvae were challenged with Aeromonas hydrophila after 5 feeding days, in order to monitor the prophylactic effect on the Csq fed larvae. The experiment started with an average initial weight of 50 ± 2 mg and total length of 12 ± 2 mm for post larval stage (40 days post hatch; dph), then continued feeding diets for a period of 20 days. Larvae survival percentage (%), mean total length (TL), width (W), total weight (TW), total weight gain (TWG), average daily weight (ADW) and specific growth rate (SGR) were recorded as morphometric measurements representing growth compared to the control groups. The results revealed that 1g Csq 100 g–1 diet at P < 0.05 was the most effective concentration that achieved higher survival percentages; 94.5 ± 0.5 and 74 ± 2.0%, increasing the specific growth rate by 7.22% and 5.77% for non challenged and challenged weaned larval groups, respectively. Otherwise, the control challenged group displayed the lowest performance in all assayed parameters with the coincidental decrease in the survival % and specific growth rates. Similarly, lower growth performance was also observed at 2 g 100 g–1 diet. Thus, the incorporation of chitosan at a level of 1g in fish diet enhanced the performance and reduced the fish mortality under stress conditions. 相似文献
In a climate variability context, knowledge about Alpha grass (Stipa tenacissima) seed germination and seedling establishment requirements is a key factor given the relevance of this species in wide areas of North Africa and southern Europe. Such knowledge can help to collect information about current S. tenacissima populations allowing the conservation and restoration in these ecosystems. According to this objective, we conducted a series of laboratory studies to investigate the effects of several ecological factors as temperature, light, salinity, burial depth and drought, in relation to germination capacity and development of new seedlings. The main results revealed that germination was delayed with increasing drought conditions generated by osmotic solutions. Seeds germinated at all the concentrations of NaCl solutions, but germination was completely inhibited at a PEG 6000 solution of ?1.6 MPa. Further, an osmotic potential of ?0.8 MPa inhibited root and shoot growth. Variations in temperature also promoted variable germination rates (GR50). The base germination temperature (Tb (50)) was less than 2 °C for the different osmotic potentials. Burial depth was another limiting factor required for establishment. Despite significant seed production, this study has identified several key limiting points affecting seed germination and seedling establishment which can affect the viability of their populations in the near future. 相似文献
The experimental work of studying the adsorption of ketotifen and allopurinol by chitosan focused on determining the solubilities
and the adsorption isotherms of the adsorbates employed in this study. The adsorption of the aforementioned compounds by chitosan
was studied using the rotating bottle method. The concentrations, both before and after the attainment of equilibrium, were
determined with the aid of a reversed-phase high-performance liquid chromatography column. The results of these studies demonstrated
that ketotifen and allopurinol are both adsorbed by chitosan. The nonlinear Langmuir-like and the Freundlich models both were
applied to the experimental data. The correlation coefficients obtained from the nonlinear Langmuir-like model were better
than those obtained from Freundlich model, suggesting that allopurinol and ketotifen interacted with certain specific binding
sites on the chitosan surface. The allopurinol adsorption experiments indicated that the particle size of chitosan and therefore
the surface area can significantly affect the Langmuir capacity constant, while the affinity constants are statistically the
same. As expected from the solubility studies, the ketotifen adsorption experiments at 2 different pHs (7 and 10) showed that
the adsorption affinity at pH 10 was much higher than at pH 7. What was not expected was that the capacity constants were
significantly different, suggesting that further studies are needed using common ion buffers and multicomponent adsorption
for the proper mechanism to be determined. 相似文献
Prediction of multicomponent adsorption is still one of the most challenging problems in the adsorption field. Many models have been proposed and employed to obtain multicomponent isotherms from single-component equilibrium data. However, most of these models were based on either unrealistic assumptions or on empirical equations with no apparent definition. The purpose of this investigation was to develop a multicomponent adsorption model based on a thermodynamically consistent equation, and to validate that model using experimental data. Three barbiturates--phenobarbital, mephobarbital, and primidone--were combined to form a ternary system. The adsorption of these barbiturates from simulated intestinal fluid (without pancreatin) by activated carbon was studied using the rotating bottle method. The concentrations, both before and after the attainment of equilibrium, were determined with a high-performance liquid chromatography system employing a reversed-phase column. The proposed equation and the competitive Langmuir-like equation were both fit to the data. A very good correlation was obtained between the experimental data and the calculated data using the proposed equation. The results obtained from the original competitive Langmuir-like model were less satisfactory. These results suggest that the proposed equation can successfully predict the trisolute isotherms of the barbituric acid derivatives employed in this study. 相似文献
This study aimed to investigate the effect of air pollution on diurnal variation of performance in anaerobic tests, cardiovascular and hematological parameters, and blood gases on soccer players following the Yo–Yo Intermittent Recovery Test Level-1 (YYIRT1). In a randomized order, 11 healthy soccer players (mean age: 21.8 [range: 20–24] years; height: 178.00 [range: 1.64–1.83] cm; body mass index [BMI]: 23.57 [range: 20.45–28.03] kg.m?2) performed a YYIRT1 at two different times of day (TOD) (08:00 h and 18:00 h) in two areas (i.e. polluted (PA) and non-polluted (NPA)) with a recovery period of ≥ 72 h in between, to determine the maximal oxygen uptake (VO2max). In each test session: resting oral temperature is measured, anaerobic performances (pre- and post-YYIRT1) were performed, cardiovascular parameters and blood samples were collected at: rest, 3 min and 60 min after the YYIRT1, to assess blood gases and hematological parameters. Our results showed that, agility performance, VO2max, red blood cells (RBC), hemoglobin (Hb), pH, and bicarbonate levels (HCO3?) decrease significantly (p < 0.001) following the YYIRT1 in PA compared to NPA. Likewise, the heart rate (HR), systolic blood pressure (SBP), platelets (PLT), white blood cells (WBC), neutrophiles (NEUT), lymphocytes (LYM), and partial pressure of CO2 levels (PvCO2) were significantly higher (p < 0.001) in PA. This effect was slightly accentuated at 18:00 h for some parameters (i.e. Agility, HCO3?, HR, PvCO2, RBC, SBP). However, performances of sprint and Sargent jump test (SJT), oral temperature, rate of perceived exertion scales (RPE), partial pressure of O2 (PvO2), diastolic blood pressure (DBP), and monocytes (MON) were not affected by pollution (p > 0.05). In conclusion, pollution seems to be critical for health stability and performance in response to YYIRT1 especially in the evening and the winter season. Therefore, coaches and athletes should draw attention to the potential importance of land use planning in their training sessions and competitions in the morning in polluted area to minimize the risk of pollution exposure. 相似文献
Cucullanus tunisiensissp. nov., (Nematoda: Cucullanidae), collected from the intestine of the white grouper Epinephelus aeneus from waters off the coast of Tunisia is described based on light and scanning electron microscopic observations. The new species is characterized by the presence of lateral alae, ventral sucker, long unequal spicules (left spicule 2474-2789 μm long, right spicule 2357-2518 μm long). This is the sixth nominal species of the genus Cucullanus Müller, 1777 and the first representative of this genus infecting fishes of Serranidae family reported from Tunisian waters.
Assessing differences in plant functional traits (PFTs) along climatic gradients is potentially useful for understanding variation within and across populations, and for predicting their responses to climate change. This study investigates the intraspecific variability of several PFTs in Stipa tenacissima (Alpha grass) seedlings from different populations distributed across a climatic gradient. Seven populations from Tunisia to Spain within a 100–600 mm/year rainfall range were selected. Seedlings from each population were grown in a common garden. We expected the functional characteristics to differ among seedling populations according to their climatic gradient. The response patterns were helpful to predict acclimation and fitness under future climatic conditions in these populations. The seedling development analysis showed differences in PFTs among S. tenacissima populations. The biomass traits analysis revealed that higher above-ground biomass was related to higher below-ground development. The leaf traits proved that seedlings with longer leaf length would have less sclerophyllous leaves, a trade-off between productivity and drought resistance. The root traits analysis reflects seedling strategies to maximize resource uptake efficiency. PFTs showed several significant relationships with climatic conditions. The less rainfall, the higher plant allocation to root systems exploring soil. Higher mean temperatures were related to reduced root/plant development. The PFT analysis proves that species followed the ‘optimal partitioning theory’, in that plants preferentially allocate biomass to acquire the resource that most limits their development. However, both the environmental conditions and genetic diversity in S. tenacissima populations influenced seedling growth and behaviour to face ongoing climate change.