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Amornrat Chumthong Mana Kanjanamaneesathian Ashara Pengnoo Ruedeekorn Wiwattanapatapee 《World journal of microbiology & biotechnology》2008,24(11):2499-2507
Endospores of B. megaterium were formulated in granule formulations with sodium alginate, lactose and polyvinylpyrrolidone (PVP K-30) by the wet granulation
technique. The granule formulation exhibited good physical characteristics, such as high-water solubility and optimal viscosity,
that would be suitable for spray application. The bacteria remained viable in the dry granule formulation at 109 c.f.u./g after 24 months storage at room temperature. Under laboratory conditions, aqueous solutions of the formulation showed
high activity against mycelial growth of R. solani (99.64 ± 0.14% mycelial inhibition). High viability of the bacterial antagonist on leaf sheath and leaf blade at day 7 after
spraying with the formulation was observed (approximately 106 c.f.u./g of plant). Application of an equivalent number of un-formulated endospores resulted in much loss of the bacterial
endospores even 1 day after application. In a small pilot field study, an aqueous solution of the formulation (3%w/v) applied
by spraying at days 1, 5 and 10 after pathogen inoculation of the rice plants was more effective in suppressing rice sheath
blight disease than one application of a fungicide (Iprodione) at day 1. Additionally, rice plants sprayed with the aqueous
solution of the granule formulation had higher panicle and whole kernel weights than those of fungicide-treated and control
(untreated) plants. 相似文献
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Ashara Pengnoo Ruedeekorn Wiwattanapattapee Amornrat Chumthong Mana Kanjanamaneesathian 《World journal of microbiology & biotechnology》2006,22(1):9-14
Summay Soil samples were taken from 48 fields in the southern part of Thailand in which either bambara groundnut (Vigna subterranea) or groundnut (Arachis hypogeae) had been planted. Bacillus spp. were isolated using soil dilution plates and heat treatment to screen for endospore-producing bacteria. Among 342 Bacillus spp. isolates tested, 168 isolates were not antagonistic to Bradyrhizobium sp. strain NC-92 using dual culture technique. Further testing found 16 isolates of Bacillus spp. had the ability to inhibit mycelial growth of Rhizoctonia solani, a causal agent of leaf blight of bambara groundnut. Among these isolates, Bacillus spp. isolate TRV 9-5-2 had the greatest activity in anti-microbial tests against R. solani. This isolate was later identified as B. firmus. A powder formulation of B. firmus was developed by mixing bacterial endospores, talcum, sodium carboxymethylcellulose (SCMC) and polyvinylpyrolidone (PVP).
The formulations contained bacterial levels ranging from 108 to 1010 c.f.u./g and the viability of bacteria in all formulations remained high after 1 year storage at room temperature (26–32 °C).
All formulations showed satisfactory effectiveness in vitro in suppressing mycelial growth of R. solani using dual culture technique. The application of formulations as seed treatment showed that these formulations did not cause
abnormality of seedling shape and had no effect on the germination of bambara groundnut seeds. 相似文献
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Kanjanamaneesathian Mana Pengnoo Ashara Jantharangsri Apinya Niratana Ladda Kusonwiriyawong Chirasak 《World journal of microbiology & biotechnology》2000,16(6):523-527
Bacterial formulations, produced using both Bacillus megaterium and B. pumilus individually with pharmaceutical technology, were formulated using a wet granular method. Viability testing in the laboratory
revealed that bacterial populations rapidly declined during storage at room temperature (26–30 °C) for 6 months. The scanning
electron microscope (SEM) was used to observe bacterial formulations. Both endospores and vegetative cells of B. megaterium and B. pumilus were detected on the formulation surfaces.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
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Ashara Pengnoo Chirasak Kusongwiriyawong Ladda Nilratana Mana Kanjanamaneesathian 《BioControl》2000,45(2):245-256
Bacterial formulations, produced using both Bacillus megaterium andB. pumilus individually with pharmaceutical technology, were testedunder both greenhouse and field conditions. In the greenhouse testing,some bacterial formulations, for instance For 7 minus Lac and For 16 minusLac, performed as well as freshly prepared bacterial antagonists insuppress sheath blight disease. In the field testing, For 16 minus Lac wasnot effective in suppressing sheath blight development. Failure of the For16 minus Lac to suppress sheath blight disease in the field trial may be dueto the dilution and inactivation of antibiotics produced by B.megaterium in the aquatic environment in the rice field and climaticconditions during the formulation application. 相似文献
5.
R. Wiwattanapatapee A. Chumthong A. Pengnoo M. Kanjanamaneesathian 《World journal of microbiology & biotechnology》2013,29(8):1487-1497
Bacillus megaterium encapsulated in calcium alginate microcapsules was prepared and tested for its efficacy against sheath blight disease of rice. In laboratory conditions, the aqueous suspension (1:100, v/v in potato dextrose agar) of the bacterial microcapsules (1010 spores/ml) inhibited mycelial growth of Rhizoctonia solani (>99 %) after the microcapsules were produced and stored for 12 months at room temperature (28 ± 2 °C). The survival of the bacterium in the microcapsules in response to ultraviolet (u.v.) irradiation and high temperature was investigated. The survivability of the bacterium in the encapsulated form was greater than that of the fresh cells when it was subjected to u.v. (20-W General electric u.v. lamp from a 25 cm distance for 48 h) and a high temperature treatment (80 °C for 48 h). Cells of the bacterium were detected by scanning electron microscope on both the leaf sheath and the leaf blade (in pot tests in a greenhouse) after spraying encapsulated product. The number of bacteria on the surface of both rice tissues (5 Log. number/g of plant) after spraying with encapsulated product was not significantly different from that after spraying with fresh cells onto the rice seedlings. Spraying the encapsulated B. megaterium on rice plants in the greenhouse was as effective as spraying a chemical fungicide for suppressing rice sheath blight disease. 相似文献
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Kanjanamaneesathian Mana Phetcharat Vasun Pengnoo Ashara Upawan Supatra 《World journal of microbiology & biotechnology》2003,19(8):825-829
Trichoderma harzianum, isolate T 01-22, was cultured on either sorghum grains, ground mesocarp fibre of oil-palm or oil-palm shell, both amended with urea fertilizer (100:1, w/w). T. harzianum cultured on ground mesocarp fibre was then used to coat seeds of Chinese kale (Brassica alboglabra Bailey) to control damping-off of seedlings caused by Pythium aphanidermatum. Biomass of T. harzianum cultured on ground mesocarp fibre of oil-palm was more effective than Captan and Benomyl, but less effective than Metalaxyl, in controlling damping-off of Chinese kale seedlings. Viability of T. harzianum growing on sorghum grains was reduced significantly during 7 months storage, followed by that of T. harzianum cultured on ground mesocarp fibre and oil-palm shell, both amended with urea fertilizer (46-0-0) at 100:1 (w/w). 相似文献
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