瘿螨科二新种记述：（蜱螨亚纲：瘿螨总科）

本文记述瘿螨科二个新种:樟小瘤瘿螨Acerimina cinnamomi Kuang和杧果瘿螨Eriophyes mangiferae Kuang et Cheng,并附有形态特征图。     

叶刺瘿螨亚科一新属三新种：真螨目：瘿螨科

新属为新诺尔瘿螨属Neoknorella Kuang et Feng,gen.nov.三新种是竹新诺尔瘿螨Neoknorella bambusae sp.nov.,竹裂柄瘿螨 Dichopelmus bambusae sp.nov.和樟无伪足瘿Anothopoda cinnamomi sp.nov.,它们均营自由生活。     

鳋科（寄生桡足类）胸板的扫描电镜比较研究

本文利用扫描电镜观察了我国鳋科的5个属的种类。首次发现在它们的胸部腹面游泳足连接板之下均有一板状结构,其形状与栉齿的着生情况在不同属之间存在着明显的差异,同属不同种之间也不尽相同,这是一个过去未曾被注意过的结构,权且称之为胸板。它是一个独立的结构,不与连接板相连。胸板上的栉齿从形态-功能-适应三位一体分析,它与附着寄主有夭,以免被水流冲走,与无栉齿的相比,应是一个进步性状。     

Cytochemical Localization of Reserves during Seed Development in Arabidopsis thaliana under Spaceflight Conditions

Successful development of seeds under spaceflight conditionshas been an elusive goal of numerous long-duration experimentswith plants on orbital spacecraft. Because carbohydrate metabolismundergoes changes when plants are grown in microgravity, developingseed storage reserves might be detrimentally affected duringspaceflight. Seed development in Arabidopsis thaliana plantsthat flowered during 11 d in space on shuttle mission STS-68has been investigated in this study. Plants were grown to therosette stage (13 d) on a nutrient agar medium on the groundand loaded into the Plant Growth Unit flight hardware 18 h priorto lift-off. Plants were retrieved 3 h after landing and siliqueswere immediately removed from plants. Young seeds were fixedand processed for microscopic observation. Seeds in both theground control and flight plants are similar in their morphologyand size. The oldest seeds from these plants contain completelydeveloped embryos and seed coats. These embryos developed radicle,hypocotyl, meristematic apical tissue, and differentiated cotyledons.Protoderm, procambium, and primary ground tissue had differentiated.Reserves such as starch and protein were deposited in the embryosduring tissue differentiation. The aleurone layer contains alarge quantity of storage protein and starch grains. A seedcoat developed from integuments of the ovule with gradual changein cell composition and cell material deposition. Carbohydrateswere deposited in outer integument cells especially in the outsidecell walls. Starch grains decreased in number per cell in theintegument during seed coat development. All these characteristicsduring seed development represent normal features in the groundcontrol plants and show that the spaceflight environment doesnot prevent normal development of seeds in Arabidopsis. Arabidopsis ; spaceflight; embryo; endosperm; seed coat; storage reserves     

kar交配法转移YACs至新宿主的研究

利用改进的kar交配法,将一个含有340kb人基因组DNA的YAC片段的供体酵母菌株YAC23与受体菌株YLB504进行交配,以选择平板对所形成的候选YAC导入菌进行筛选。经PCR分析,候选YAC导入菌在404bp处有一个扩增带,即具有受体菌株的交配型(MAT α)。进一步用脉冲电泳进行核型鉴定,证实YACs己成功地进入受体,实现了YACs从一个宿主到另一个宿主之间的转移。     

S－美芬妥英羟化代谢多态性的分子机制研究进展

人群中存在着Ｓ－美芬妥英羟化代谢多态性。从人肝微粒体已分离出Ｓ－美芬妥英羟化酶。在基因克隆研究中已分离出与该羟化酶活性有关的Ｐ４５０ｃＤＮＡ,属Ｐ４５０２Ｃ１９。最近报道,人肝中Ｐ４５０２Ｃ１９的含量和催活Ｓ－美芬妥英羟化活性密切相关,其弱代谢者主要由于ＣＹＰ２Ｃ１９的外显子５单碱基对（Ｇ→Ａ）的变异,使核苷酸序列错位而产生无功能的Ｐ４５０蛋白的结果。     

石榴小爪螨在樟树上的生物学特性和种群动态

石榴小爪螨 Oligonychus punicae(Hirst)是鳄梨、石榴、葡萄和樟树上的重要害螨。在江西弋阳以卵越冬,属兼性滞育,发育起始温度为7.9℃,完成一代的有效积温为205.5日度。室内生命表结果是,在 20.7℃和28℃下r_m分别为0.42和0.44,R₀为37.58和19.07。一年内种群数量动态的总趋向是,在干旱季节食螨瓢虫和一种钝绥螨是石榴小爪螨数量增长的限制因子,而在雨季,特别是连续暴雨就成为螨量急剧下降的主导因素。     

人嗜中性白细胞活化蛋白-1/白细胞介素-8合成基因的修正及修正基因在大肠杆菌中的表达

本文采用寡核苷酸介导的定位诱变技术修正了人嗜中性白细胞活化蛋白-1/白细胞介素-8合成基因中出现的合成错误,在该基因编码区的201位插入了原来缺失的G残基,从而使之恢复正确读框。经对修正基因进行DNA全序列测定,表明定位诱变的结果符合设计要求。在此基础上,利用原核高效表达载体pBV220在P_RP_L串联启动子的控制下在大肠杆菌中对该基因进行了表达,并测定了重组人嗜中性白细胞活化蛋白-1/白细胞介素-8的嗜中性白细胞趋化活性。本工作为开展人嗜中性白细胞活化蛋白-1/白细胞介素-8基因工程及蛋白质工程的研究奠定了基础。     

瘤瘿螨属四新种一新亚种记述(蜱螨亚纲:瘿螨科)

本文记述的四个新种是木樨瘤瘿螨Aceria osmanthis Kuang,燕麦瘤瘿螨Aceria avenae Kuang et Zhang,江苏瘤瘿螨Aceria jiangsunensis Kuang和昆明瘤瘿螨Aceria kunmiaensis Kuang et Hong,新亚种是中华柑桔瘤瘤螨Aceria sheldoni chinensis Kuang et Hong。