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Cai Wen-Qi Fang Rong-Xiang Shang Hong-Sheng Wang Xu Zhang Feng-Li Li Yue-Ren Zhang Jiu-Chun Cheng Xiao-Ying Wang Gui-Ling Mang Ke-Qiang 《Molecular breeding : new strategies in plant improvement》2003,11(1):25-35
Both cucumber mosaic virus (CMV) and tobacco mosaic virus (TMV) coat protein (CP) genes have been transferred to chilli pepper (Capsicum annuum var. Longunt) cultivar 8212 by a modified procedure of Agrobacterium tumefaciens-mediated transformation using hypocotyl as the explant. PCR analysis revealed the presence of both CMV and TMV CP genes in at least 11 primary transformants out of 49 kanamycin-resistant chili pepper plants. Ten T1 lines from five independent transformation events were identified as putative homozygous transgenic lines based on the rooting assay of their T2 seedlings on the kanamycin-containing media. Integration and expression of CMV CP and TMV CP transgenes in one of the homozygous line, 16-13, were confirmed bySouthern blot, RT-PCR and western blot analyses. Line 16-13 was highly resistant to infection of homologous CMV and TMV strains in greenhouse conditions when successively challenged with CMV and TMV or challenged with TMV alone.Futhermore, field trials on T2, T3 and T4 progenies of Line 16-13 were performed on scales of 123, 300 and 10,000 plants, respectively, in consecutive years 1996, 1997 and 1998 with the permission of the Chinese government authority. The transgenic plants displayed delayed symptom development and significantly milder disease severity in field conditions when compared to untransformed chili pepper plants, resulting in 47 and 110% increase in pepper fruit yield in surveys conducted in 1997 and 1998 trials, respectively. Finally, quality analysis and biosafety assesment were performed on transgenic chili pepper fruit concurrently with the control fruit, and demonstrated that the transgenic chili pepper fruit is substantially equivalent to the non-transgenic pepper in terms of the quality and biosafety when consumed as a food additive. 相似文献
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通过研究同一种群雌性宽肋盘腹蛛Cyclocosmia latusicosta Zhu,Zhang & Zhang,2006,发现该种个体间在体长、步足长度、齿堤齿的形态与数量、圆盘肋数及纳精囊长宽之比等方面变异较大;宽肋盘腹蛛与里氏盘腹蛛Cyclocosmia ricketti(Pocock,1901)的区别主要是圆盘印痕中央是否存在纽扣状突起. 相似文献
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