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1.
2.
Big, beautiful organisms are useful for biological education, increasing evolution literacy, and biodiversity conservation. But if educators gloss over the ubiquity of streamlined and miniaturized organisms, they unwittingly leave students and the public vulnerable to the idea that the primary evolutionary plot of every metazoan lineage is “progressive” and "favors" complexity. We show that simple, small, and intriguingly repulsive invertebrate animals provide a counterpoint to misconceptions about evolution. Our examples can be immediately deployed in biology courses and outreach. This context emphasizes that chordates are not the pinnacle of evolution. Rather, in the evolution of animals, miniaturization, trait loss, and lack of perfection are at least as frequent as their opposites. Teaching about invertebrate animals in a “tree thinking” context uproots evolution misconceptions (for students and the public alike), provides a mental scaffold for understanding all animals, and helps to cultivate future ambassadors and experts on these little‐known, weird, and fascinating taxa. 相似文献
3.
Jesse L. Steinfeld 《The Western journal of medicine》1984,141(6):878-883
Although cigarette smoking is the number one public health problem in the United States, physicians have failed to take the lead either in convincing youngsters not to begin smoking or in aiding adults to quit smoking. To be most effective and convincing in combating the smoking epidemic, practicing physicians must have the same basic fund of knowledge about the short- and long-term consequences of smoking as they do about other commonly encountered medical problems. By acting on such knowledge and adopting a definite set of attitudes and activities in their offices and with patients, physicians can make a significant contribution to their patients and to the entire community in which they practice. 相似文献
4.
1. The apparent acid and basic dissociation constants were determined potentiometrically by the methods of hydrolysis and of titration for the following ampholytes: Glycocoll, glycylglycocoll, alanylglycocoll, valylglycocoll, leucylglycocoll, methylleucylglycocoll, phenylalanylglycocoll and glycylglycylglycocoll. The constants were also determined in the presence of KCl and of K2SO4 at equal ionic strength. 2. In general, the relative order of magnitude of the constants decreased as the number of carbon atoms between amino and carboxyl groups increased. An explanation of this is offered on the basis of theories of electronic structure. 3. The application of the modern concepts of solutions to the case of the ampholytic ions is discussed. The inadequacy of the present theories is pointed out. 4. The constants were found, in general, to be functions of the hydrogen ion activity and the ionic strength of the solutions. Apparent contradictions to the Debye-Hückel theory are pointed out and partially explained on the basis of specific ion effects. 相似文献
5.
D Poland 《Biopolymers》1999,50(7):720-732
A statistical mechanical model is given for linear associating systems that contain defects, using the double-stranded actin polymer as an example. We treat the system as a one-dimensional lattice that can desorb monomers (giving defects) using grand partition function techniques. The main difference from a standard adsorption problem is that the monomer units are also responsible for the structural integrity of the lattice (polymer) and if too many desorb the polymer will be broken. We use literature data to estimate the density of defects in the actin polymer. 相似文献
6.
D Poland 《Biophysical chemistry》2001,94(3):185-199
In the present paper we show how one can use the perturbation of some molecular optical property (for example circular dichroism or chemical shift) as a function of concentration to construct cluster distribution functions describing the self-association of molecules in solution. The optical data are first converted into data giving the variation of the average extent of clustering as a function of the total concentration and then, using straightforward thermodynamics, a set of moments of the cluster distribution function can be obtained. Utilizing the maximum-entropy method, the moments are then used to calculate approximate distribution functions, where the more moments that are used the better the approximation obtained. Given the probability distribution for clusters of different sizes one can then calculate the equilibrium constant for each stage of association. Thus one converts average degree of association into equilibrium constants without having to use any specific model. By this method one can clearly tell whether the equilibrium constants remain constant, increase, or decrease with the number of molecules in a cluster. We apply the method to literature data for two systems, namely daunorubicin, which has a strong tendency to cluster in solution, and Mg(ATP)(2-) which forms weaker clusters. We find that the successive equilibrium constants for adding a monomer to a cluster are approximately constant for daunorubicin but clearly decrease as a function of increasing cluster size for Mg(ATP)(2-). 相似文献
7.
Thomas M. Becker Jack D. Poland Thomas J. Quan Mark E. White Jonathan M. Mann Allan M. Barnes 《The Western journal of medicine》1987,147(5):554-557
Meningitis caused by Yersinia pestis developed in 6 (6%) of a total of 105 patients with plague reported to the Centers for Disease Control from 1970 to 1979. Five of the six cases occurred in children aged 10 to 15 years. All six patients received antibiotic therapy before meningitis developed, which appeared between the 9th and 14th days after the onset of acute illness in five of the six patients. There were no neurologic sequelae. The antigenic and biochemical profiles of the Y pestis strains isolated from cerebrospinal fluid in the meningitis cases did not differ from those of the Y pestis strains obtained from blood and bubo aspirates in the other 99 patients, and neither did in vitro studies suggest antibiotic resistance. While plague meningitis is an uncommon complication of acute plague infection, physicians in the western United States should be aware that it may develop as much as 14 days after antibiotic therapy for the acute plague infection has been initiated. 相似文献
8.
Rakesh C. Kukreja Robert L. Jesse Michael L. Hess 《Molecular and cellular biochemistry》1992,111(1-2):17-24
The purpose of this study was to explore the role of singlet oxygen in cardiovascular injury. To accomplish this objective, we investigated the effect of singlet oxygen [generated from photoactivation of rose-bengal] on the calcium transport and Ca2+-ATPase activity of cardiac sarcoplasmic reticulum and compared these results with those obtained by superoxide radical, hydrogen peroxide and hydroxyl radical. Isolated cardiac SR exposed to rose bengal (10 nM) irradiated at (560 nm) produced a significant inhibition of Ca 2+ uptake; from 2.27 ± 0.05 to 0.62 ± 0.05 µmol Ca+/mg.min (mean ± SE) (P < 0.01) and Ca2+-ATPase activity from 2.08 ± 0.05 µmol Pi/min. mg to 0.28 ± 0.04 µmol Pi/min. mg (mean ± SE) (P < 0.01). The inhibition of calcium uptake and Ca2+-ATPase activity by rose bengal derived activatedoxygen (singlet oxygen) was dependent on the duration of exposure and intensity of light. The singlet oxygen scavengers ascorbic acid and histidine significantly protected SR Ca2+-ATPase against rose bengal derived activated oxygen species but superoxide dismutase and catalase did not attenuate the inhibition. SDS-polyacrylamide gel electrophoresis of SR exposed to photoactivated rose bengal up to 14 min, demonstrated complete loss of Ca2+-ATPase monomer band which was significantly protected by histidine. Irradiation of rose bengal also caused an 18% loss of total sulfhydryl groups of SR. On the other hand, superoxide (generated from xanthine oxidase action on xanthine) and hydroxyl radical (0.5 mM H2O2 + Fe2+ -EDTA) as well as H2O2 (12 mM) were without any effect on the 97,000 dalton Ca2+-ATPase band ofsarcoplasmic reticulum. The results suggest that oxidative damage of cardiac sarcoplasmic reticulum may be mediated by singlet oxygen. This may represent an important mechanism by which the oxidative injury to the myocardium induces both a loss of tension development and arrhythmogenesis. 相似文献
9.
10.
Photoaffinity labeling of the Ah receptor 总被引:6,自引:0,他引:6
A series of halodibenzo-p-dioxins with the photolabile aryl azide functional group were synthesized and screened as potential photoaffinity labels for the Ah receptor, and 2-azido-3-iodo-7,8-dibromodibenzo-p-dioxin was selected for radiosynthesis with 125I (specific activity 2176 Ci/mmol, equilibrium dissociation constant, KD = 0.76 nM). Following incubation of this 125I-labeled photoaffinity ligand with the protamine sulfate-precipitated fraction of C57BL/6J mouse liver cytosol, and irradiation with long wavelength ultraviolet light, the radiolabeled macromolecules were precipitated with acetone and analyzed by denaturing gel electrophoresis and autoradiography. Among the labeled products, two peptides with apparent molecular masses of 95,000 and 70,000 daltons had the following properties: 1) they were selectively labeled at low ligand concentrations; 2) they were labeled in approximately a 1:1 ratio; 3) co-incubation with receptor agonists inhibited the photoaffinity labeling of both peptides to a similar extent, and structure activity relationship for inhibition of labeling by these agonists corresponded to that for their binding affinity to the Ah receptor; 4) upon nondenaturing chromatographic separation of photoaffinity labeled cytosol on high performance liquid chromatography size exclusion and anion exchange columns, the 95- and 70-kDa peptides coelute; 5) the migration of these peptides upon denaturing electrophoresis is the same in the presence or absence of a thiol reducing agent; and 6) proteolysis of the 95- and 70-kDa peptides produces a similar pattern of cleavage peptides. The simplest structure of the Ah receptor in mouse liver cytosol, appears to be a dimer composed of two noncovalently linked subunits of apparent molecular masses of 95 and 70 kDa, which have homologous structure and similar ligand binding sites, but other possibilities are discussed. 相似文献