全文获取类型
收费全文 | 1995篇 |
免费 | 75篇 |
国内免费 | 1篇 |
出版年
2021年 | 10篇 |
2020年 | 11篇 |
2019年 | 14篇 |
2018年 | 23篇 |
2017年 | 17篇 |
2016年 | 42篇 |
2015年 | 69篇 |
2014年 | 62篇 |
2013年 | 106篇 |
2012年 | 101篇 |
2011年 | 130篇 |
2010年 | 74篇 |
2009年 | 65篇 |
2008年 | 111篇 |
2007年 | 120篇 |
2006年 | 124篇 |
2005年 | 103篇 |
2004年 | 85篇 |
2003年 | 120篇 |
2002年 | 96篇 |
2001年 | 16篇 |
2000年 | 20篇 |
1999年 | 25篇 |
1998年 | 26篇 |
1997年 | 13篇 |
1996年 | 18篇 |
1995年 | 25篇 |
1994年 | 18篇 |
1993年 | 14篇 |
1992年 | 23篇 |
1991年 | 28篇 |
1990年 | 23篇 |
1989年 | 25篇 |
1988年 | 14篇 |
1987年 | 16篇 |
1986年 | 21篇 |
1985年 | 17篇 |
1984年 | 14篇 |
1983年 | 19篇 |
1982年 | 12篇 |
1981年 | 16篇 |
1980年 | 17篇 |
1979年 | 10篇 |
1978年 | 14篇 |
1977年 | 18篇 |
1976年 | 9篇 |
1975年 | 15篇 |
1973年 | 9篇 |
1969年 | 9篇 |
1966年 | 8篇 |
排序方式: 共有2071条查询结果,搜索用时 15 毫秒
1.
2.
A robust method for selection of variables with the greatest discriminatory power is presented in the paper. The method deals with the two groups of data problem. An application of the method to some respiratory disease data and comparisons with classical procedures are given, also. 相似文献
3.
4.
5.
H.-Dietmar Behnke 《Nordic Journal of Botany》1991,11(3):333-344
The phloem of the Myristicaceae is composed of sieve elements, parenchymatous cells, and fibers. Within the metaphloem and secondary phloem parenchymatic layers including prominent secretory elements alternate with tangential bands of fibers and layers composed of sieve elements, companion cells and phloem-parenchyma cells. among the latter the sieve elements are most abundant and easily identified by the presence of thick (nacreous) walls. The most characteristic feature of the sieve elements of Myristicaceae (and found nowhere else among the Magnoliiflorae) are nuclear crystals, which are released into the lumen during nuclear degeneration and persist in the mature cell. P-and S-type sieve-element plastids were recorded for the 18 species investigated. Both types of the plastid are characterized by large diameters and many medium-sized starch grains. The sizes and contents (small protein crystals only) of the P-type plastids of the Myristicaceae do not conform to the tiny P-type plastids (with large protein crystals) of the Annonaceae, a family to which the Myristicaceae is traditionally allied. 相似文献
6.
Jerzy Chelkowski 《Mycotoxin Research》1987,3(1):3-6
14C-roquefortine, given to ratsper os or intraperitoneally, was eliminated principally by the biliary route. Most of the given radiolabel accounted for had been voided within one day of administration. Roquefortine was not metabolised in rat liver homogenate but significant transformation to more polar products was evident in faeces. 相似文献
7.
Jerzy Chelkowski Piotr Zajkowski Marcin Zawadzki Juliusz Perkowski 《Mycotoxin Research》1987,3(1):25-32
An isolated rat liver was perfused with deoxynivalenol (DON) at a dose of 3 mg in a recirculating perfusion system. To identify glucuronide conjugates equal amounts of bile samples, perfusate and liver homogenates were incubated with and without (control) a β-glucuronidase preparation and analyzed by thin layer chromatography and capillary gas liquid chromatography — chemical ionization mass spectrometry. A total of 40.4% of the administered dose of DON was found to be conjugated with glucuronic acid (perfusate 20.4%, bile 19.2%, liver 0.8%), while only 1.3% of the parent DON (perfusate 1.1%, bile 0.2%) was detected. The cleavage of DON-glucuronide was demonstrated by incubating DON-glucuronide containing bile samples with intestine contents under anaerobic conditions. 相似文献
8.
Summary
Nocardia mediterranei strain LBG A3136 contains the 23.7 kb element pMEA100 in a chromosomally integrated form as well as in the free state (Moretti et al. 1985). The integrated form of this element can be excised precisely from the Nocardia chromosome without any accompanying rearrangements in flanking chromosomal DNA. After transfer into plasmid-free mutant strains, pMEA100 reintegrates site specifically into its original chromosomal locus. The exact mapping of the pMEA100 integration site was accomplished by restriction analysis and DNA sequencing. The attachment site of pMEA100, the junctions of its integrated form and plasmid-free chromosomal DNA of N. mediterranei contain an identical 47 bp long sequence which is probably required for site-specific recombination connected with integration and excision of pMEA100. Only one such sequence was found in the chromosome of pMEA100-free N. mediterranei derivatives as suggested by the single integration locus. 相似文献
9.
The role of aromatic side chain residues in micelle binding by pancreatic colipase. Fluorescence studies of the porcine and equine proteins. 下载免费PDF全文
Fluorescence techniques have been employed to study the interaction of porcine and equine colipase with pure taurodeoxycholate and mixed micelles. Nitrotyrosine-55 of porcine colipase is obtained by modification with tetranitromethane (low excess, in the presence of taurodeoxycholate) of the protein followed by gel filtration and ion-exchange chromatography. Verification of the residue modified was obtained by h.p.l.c. peptide purification and sequence analysis. Reduction and quantitative reaction with dansyl chloride yields a fluorescent derivative that is twice as active in conjunction with lipase as is native colipase and that exhibits a strong emission band at 550 nm. Addition of micellar concentrations of taurodeoxycholate causes a 4.3-fold increase in the emission maximum as well as a 70 nm blue shift to 480 nm. Inclusion of oleic acid to form a mixed micelle reduces these spectral effects. Scatchard analysis of the data yield a Kd of 6.8 X 10(-4) M and a single colipase-binding site for taurodeoxycholate micelles. The data, by analogy to a phospholipase system, are consistent with a direct insertion of dansyl-NH-tyrosine-55 into the micelle. The presence of a single tryptophan residue (Trp-52) in equine colipase provides an intrinsic fluorescent probe for studying protein-micelle interaction. The emission maximum of horse colipase at 345 nm indicates a solvent-accessible tryptophan residue which becomes less so on binding of micelles. A blue shift of 8 nm and a 2-fold increase in amplitude is indicative of a more hydrophobic environment for tryptophan induced by taurodeoxycholate micelles. There is also a decrease in KSV for acrylamide quenching in the presence of micelles, which further supports a loss of solvent accessibility. The most dramatic pH effects are observed with KI quenching, and may indicate the presence of negative charges near Trp-52. 相似文献
10.
Jerzy S Nowak 《Journal of genetics》1987,66(2):133-138
Erythrocytes from healthy men were examined for the presence of complement (C3b) receptor using haemagglutination assay with human aggregated IgG (aggIgG) and guinea pig complement. The results were expressed as the intensity of haemagglutination that corresponded to the C3b receptor sites density as evidenced by radioimmunobinding results. Among normal men three phenotypes of complement receptor (CR) were distinguished: high (CRh/CRh) phenotype corresponding to strong agglutination, an intermediate (CRh/CRI) producing weak agglutination and low phenotype (CRI/CRI) that gave no agglutination. In a population of 517 normal men these three phenotypes occurred in 63.8, 30.6 and 5.6%, respectively. Frequencies of the genes responsible for high (CRh) and low (CRI) expression of erythrocyte C3b receptor were 0.791 and 0.209, respectively. 相似文献