排序方式: 共有2条查询结果,搜索用时 0 毫秒
1
1.
Jayarajapazham Rajaselvam Natarajan Benit Saqer S. Alotaibi M.A. Rathi Srisesharam Srigopalram Gurupatham Devadhasan Biji Ponnuswamy Vijayaraghavan 《Saudi Journal of Biological Sciences》2021,28(8):4117-4123
A fibrinolytic protease secreting producing Bacillus amyloliquefaciens strain KJ10 was initially screened from the fermented soybean. Maximum productivity was obtained in the culture medium after 40 h incubation, 34 °C incubation temperature at pH 8.0. Fibrinolytic protease production was enhanced in the culture medium with 1% sucrose (3712 ± 52 U/mL), 1% (w/v) yeast extract (3940 ± 28 U/mL) and 0.1% MgSO4 (3687 ± 38 U/mL). Enzyme was purified up to 22.9-fold with 26%recovery after Q-Sepharose HP column chromatography. After three steps purification, enzyme activity was 1606U/mg and SDS-PAGE analysis revealed 29 kDa protein and enzyme band was detected by zymograpy. Enzyme was highly active at pH 8.0, at wide temperature ranges (40 °C ? 55 °C) and was activated by Mn2+ (102 ± 3.1%) and Mg2+ (101.4 ± 2.9%) ions. The purified fibrinolytic enzyme was highly specific against N-Suc-Ala-Ala-Pro-Phe-pNA (189 mmol/min/mL) and clot lytic activity reached 28 ± 1.8% within 60 minin vitro. The purified fibrinolytic enzyme showed least erythrocytic lysis activity confirmed safety to prevent various health risks, including hemolytic anemia. Based on this study, administration of fibrinolytic enzyme from B. amyloliquefaciens strain KJ10 is safe for clinical applications. 相似文献
2.
Effect of Early Introduction of Microencapsulated Diet to Larval Atlantic Halibut, Hippoglossus hippoglossus L. Assessed by Microarray Analysis 总被引:1,自引:0,他引:1
H. M. Murray S. P. Lall R. Rajaselvam L. A. Boutilier R. M. Flight B. Blanchard S. Colombo V. Mohindra M. Yúfera S. E. Douglas 《Marine biotechnology (New York, N.Y.)》2010,12(2):214-229
An experimental microdiet prepared using an internal gelation method was used to partially replace the traditional live feed
(Artemia) for larval Atlantic halibut, Hippoglossus hippoglossus L. Three trials were conducted with microdiet introduced at 20, 32, and 43 days post first feeding and larvae were sampled
at approximately 2, 13, 23, and 33 days after microdiet introduction in each trial. The success of feeding was assessed by
morphometrics and histological analysis of gut contents. Microdiet particles were readily consumed after a period of adaptation
and provided an adequate source of nutrients with no significant increase in mortality in the microdiet-fed group compared
to the control group. However, growth was limited and there was an increased incidence of malpigmentation of the eye and skin.
Subtle changes in underlying digestive and developmental physiology were revealed by microarray analysis of RNA from control
and experimental fish given microdiet from day 20 post first feeding. Fifty-eight genes were differentially expressed over
the four sampling times in the course of the trial and the 28 genes with annotated functions fell into five major categories:
metabolism and biosynthesis, cell division and proliferation, protein trafficking, cell structure, and stress. Interestingly,
several of these genes were involved in pigmentation and eye development, in agreement with the phenotypic abnormalities seen
in the larvae. 相似文献
1