全文获取类型
收费全文 | 1184篇 |
免费 | 66篇 |
出版年
2022年 | 8篇 |
2021年 | 25篇 |
2020年 | 14篇 |
2019年 | 12篇 |
2018年 | 20篇 |
2017年 | 21篇 |
2016年 | 34篇 |
2015年 | 41篇 |
2014年 | 39篇 |
2013年 | 55篇 |
2012年 | 70篇 |
2011年 | 84篇 |
2010年 | 54篇 |
2009年 | 43篇 |
2008年 | 67篇 |
2007年 | 82篇 |
2006年 | 74篇 |
2005年 | 63篇 |
2004年 | 67篇 |
2003年 | 57篇 |
2002年 | 55篇 |
2001年 | 13篇 |
2000年 | 14篇 |
1999年 | 12篇 |
1996年 | 6篇 |
1992年 | 10篇 |
1991年 | 16篇 |
1990年 | 12篇 |
1988年 | 5篇 |
1987年 | 14篇 |
1986年 | 11篇 |
1985年 | 17篇 |
1984年 | 7篇 |
1983年 | 8篇 |
1982年 | 4篇 |
1980年 | 3篇 |
1979年 | 12篇 |
1978年 | 7篇 |
1977年 | 8篇 |
1976年 | 5篇 |
1975年 | 7篇 |
1974年 | 7篇 |
1973年 | 9篇 |
1972年 | 10篇 |
1971年 | 5篇 |
1970年 | 4篇 |
1969年 | 3篇 |
1968年 | 5篇 |
1967年 | 4篇 |
1966年 | 3篇 |
排序方式: 共有1250条查询结果,搜索用时 15 毫秒
1.
2.
A L Liozner E N Stepanova N A Volynskaia G K Vorkunova A G Bukrinskaia 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》1985,(3):91-96
A test system permitting the detection of influenza virus protein M at a concentration of 0.1-0.5 ng/ml in ELISA has been developed. The use of this system made it possible to detect influenza viruses A and B directly in crude virus-containing material and clinical samples obtained from influenza patients. During the outbreak of influenza in the spring of 1983 ELISA was successfully used for the rapid diagnosis of influenza, and some of its advantages in comparison with the conventional immunofluorescence test were thus demonstrated. To overcome difficulties arising from the low immunogenic potency of protein M, in the process of obtaining diagnostic sera and ascitic fluids the animals were immunized with the conjugate of protein M and polyelectrolite, which ensured considerable activation of humoral immune response. 相似文献
3.
4.
A mathematical model of autoimmunity is developed. This model is a system of two nonlinear differential equations, which describe the concentration dynamics of tissue cells and agressive lymphocytes. An analysis of the solutions shows that this model reproduces general behaviour of autoimmune diseases. 相似文献
5.
S V Prozorovski? F M Kirillova L K Stepanova A L Gorelov G A Levina 《Zhurnal mikrobiologii, epidemiologii, i immunobiologii》1985,(4):24-26
The antigenic structure of the L-forms of salmonellae (S. typhimurium and S. typhi) in comparison with that of the initial bacterial cultures and revertant cultures was studied with the use of the immunoferritin method. The L-forms of salmonellae were found to retain an insignificant amount of O-antigen, as well as to have K-antigen on the surface of the cytoplasmic membrane, but in a lesser amount than the initial strains. In the cultures reverting from the L-forms of S. typhimurium and S. typhi the quantitative and qualitative characteristics of O- and K-antigens were completely restored. 相似文献
6.
L I Stepanova S V Kotelevtsev P G Komarov K N Novikov V M Glazer 《Nauchnye doklady vysshe? shkoly. Biologicheskie nauki》1985,(9):27-32
The content of cytochrome P450 and monooxygenase activity has been studied in the liver of Baikal fishes (Coregonus automnalis, Thymallus articus, Brachymystax lenok and Cottocomphorus greminsky). The administration of 3-methylcholanthrene increases considerably the level of metabolic activity of microsomal fraction and cytochrome P450 content in liver. The data of microsomal fractions of rats and fishes liver electrophoresis have shown that xenobiotic causes the synthesis of similar according to the molecular weight forms of cytochrome P450 in these animals. The induction of microsomal monooxygenase inhibits the lipid peroxidation of microsomal fraction. 相似文献
7.
The survival rate of Mycobacterium lacticolum and the proportion between its R, S and M variants were studied in the course of 12 months under different conditions of storage (freeze-drying, under vaseline oil, in 0.85% NaCl solution and in distilled water). A high survival rate of the variants was found in cells freeze-dried in a protective medium containing 10% of sucrose +1% of gelatin as well as in a 0.85% solution of NaCl.The survival rate of te variants differed by 2--3 orders of magnitude in cells freeze-dried with sodium glutamate or suspended in distilled water. The proportion between the R, S and M variants in the population noticeable changed after storage under these conditions. 相似文献
8.
Intracellular Synthesis of Myxovirus Neuraminidase in Chick Embryo Cell Monolayer Culture I. Neuraminidase Activity, Hemagglutinin Synthesis, and Content of Cell-bound Sialic Acid in Newcastle Disease Virus-infected Cells
下载免费PDF全文
![点击此处可从《Journal of virology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Neuraminidase (Nase) activity of chick embryo monolayer cell homogenates was determined by its rate of splitting of neuraminlactose, free neuraminic acid (NA) being determined by the thiobarbituric acid assay. Noninfected cells were found to have no detectable amount of Nase activity. Newcastle disease virus (NDV)-infected cells (multiplicity of infection, 20 to 75 plaque-forming units per cell) displayed a high level of Nase synthesis, the rate of synthesis being parallel to that of hemagglutinin (HA) synthesis (with a 1.5 hr delay in the latter). An "eclipse" of the Nase and HA activities associated with the virus that was adsorbed onto cells was observed. The data provide evidence that the Nase is not incorporated into the viral envelope from a pre-existing cell supply but that its synthesis is coded by the viral genome. The content of cell-bound sialic acid, determined simultaneously in infected-cell homogenates, showed characteristic features allowing certain conclusions concerning the renewal of NA-terminating cell receptors during the course of infection, and the intracellular action of the Nase of the virus introduced into cells by the inoculum and that of the newly synthesized Nase at different stages of infection. 相似文献
9.
The nervous system of Tricladida. II. Neuroanatomy ofDugesia tigrina (Paludicola,Dugesiidae): An immunocytochemical study 总被引:2,自引:0,他引:2
10.
Marina S. Trofimova Inna M. Zhestkova Valentina P. Kholodova Igor M. Andreev Evgeny M. Sorokin Anna G. Kruglova Vladimir V. Kuznetsov 《Physiologia plantarum》2003,118(2):232-239
The osmotic water permeability ( P os ) of cell membranes isolated from leaves of 40-, 50- and 60-day-old Mesembryanthemum crystallinum plants was estimated by measuring light-scattering kinetics using stopped-flow spectrophotometry. The measurements were performed on the plasma membrane (PM), purified tonoplast (TP), and TP-enriched vesicles. The PM and TP-enriched vesicles were obtained by partitioning the microsomal fraction in an aqueous polymer two-phase system, whereas the purified TP vesicles were prepared by microsomal vesicle flotation on a sucrose cushion. The P os of isolated membranes declined with plant age. The kinetic experiments showed that there was no difference between the P os of the PM and TP isolated from plants of all ages. A 24-h exposure of plants to 400 m M NaCl caused a decline in the P os as well. These findings suggest that, during M. crystallinum transition to CAM, which was induced by plant ageing or salinity, plant osmoregulatory responses included changes in the P os of the leaf-cell membranes. These variations in the P os are discussed in the context of adaptive mechanisms responsible for the maintenance of the water balance in the common ice plant. 相似文献