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Cloning of genes for a hemolytic factor of Leptospira interrogans serovar autumnalis strain Congo 21-543 总被引:1,自引:0,他引:1
A DNA fragment encoding a hemolytic factor was cloned from the parasitic spirochete Leptospira interrogans serovar autumnalis strain Congo 21-543. Initial clones were isolated by screening a genomic library in pBR322 in Escherichia coli for hemolytic activity. Hemolytic activity was coded by a 4.5 kilobase BamHI-HindIII fragment. Southern hybridization with DNAs from other strains of Leptospira using this gene as a probe showed that DNAs from non-parasitic strains failed to hybridize with the probe, whereas those from all parasitic strains tested had the sequence which hybridize to the probe. 相似文献
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Microfibers present in surface structure of Leptospira. 总被引:2,自引:0,他引:2
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The number of large ribosomal RNA genes in Leptospira interrogans and Leptospira biflexa 总被引:3,自引:0,他引:3
We determined the number of large ribosomal RNA genes in five strains of Leptospira by hybridization of 15 restriction endonuclease digests of genomic DNA to the [32P]-labeled fragment of 23s rRNA gene. Almost all the restriction gels gave two radioactive bands. The conclusion from these results is that there are at least two rRNA genes in these leptospiral strains. Furthermore, the hybridization patterns of L. icterohaemorrhagiae strains Ictero No. I and RGA are almost identical. The number of rRNA genes and taxonomic relationships of these leptospires were discussed. 相似文献
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Minoru Niimoto Takao Hattori Ichiji Ito Ryuichiro Tamada Kiyoshi Inokuchi Kunzo Orita Hisashi Furue Nobuya Ogawa Tomohiro Toda Motonosuke Furusawa Shigemasa Koga Isamu Hashimoto Tatsuhei Kondo Shigeru Fujimoto Yuzuru Sugiyama Osahiko Abe Masaaki Oya 《Cancer immunology, immunotherapy : CII》1984,18(1):13-18
Summary The usefulness of LMS in postoperative immunochemotherapy of gastric cancer was investigated. In compliance with the protocol, MMC was given at a dose of 20 mg on the day of gastrectomy, and an additional 10 mg on the next day IV. The patients receiving 600 mg Tegafur daily were then divided into two groups according to whether LMS was also given or not. LMS was administered for 3 days before the operation in a daily dose of 150 mg and for 1 year or more after operation according to a schedule of 3 days' administration followed by an 11-day interval. The 2-year follow-up demonstrated that in stage III patients, the LMS (+) regimen was superior to the LMS (–) regimen, since the former prolonged the relapse-free interval significantly. The survival rate for stage III disease was also significantly higher in the LMS (+) than in the LMS (–) group. There was no significant difference in the incidence of subjective or objective side-effects between two groups. The incidence of agranulocytosis was comparable in the two groups.Gastrointestinal Cancer Research Group, Japan Levamisole Research AssociationChairmen of the Gastrointestinal Cancer Research Group, Japan LMS Research AssociationController of the Gastrointestinal Cancer Research Group, Japan LMS Research AssociationMembers of the Data Collection and Analysis SubcommitteeThis study was carried out by the Gastrointestinal Cancer Research Group, Japan LMS Research Association (directed by Prof. Kiyoshi Inokuchi, Dept. of Surgery, Kyushu University and Prof. Eiro Tsubura, Dept. of Internal Medicine, Tokushima University). The results were presented in part at the 19th General Meeting of the Japanese Society for Gastroenterological Surgery in February, 1982 相似文献
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The presence of glucosephosphate isomerase, one of the key enzymes in carbohydrate metabolism, was confirmed for the first time in the cell-free extract of Leptospira biflexa. The glucosephosphate isomerase of L. biflexa was heat-labile and its optimum pH was about 8.5. The enzyme showed an optimal temperature of about 45 C but was more stable at 30 C. Km value of the enzyme was 5.6 X 10(-3)M. The activity of the enzyme was inhibited by the inhibitor, 6-phosphogluconate. From this study, the presence of a metabolic pathway, the phosphogluconate pathway, other than non-oxidative pentose phosphate pathway presented by Baseman and Cox was suggested. 相似文献
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Ichiji Yamashita Yoshiro Nemoto Seiji Yoshikawa 《Bioscience, biotechnology, and biochemistry》2013,77(11):2231-2235
Strawberry seeds are shown to contain at least two alcohol dehydrogenases; one is NAD specific and reacts with ethanol and allyl alcohol, and the other is NADP specific and reacts with benzyl alcohol and geraniol. These two alcohol dehydrogenases were distinguished on disc electrophoresis. Their properties were different each other in ammonium sulfate fractionation, optimum reaction pH and thermostability. 相似文献
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Ichiji Tasaki 《The Journal of general physiology》1963,46(4):755-772
The permeability of the squid axon membrane was determined by the use of radioisotopes of Na, K, Ca, Cs, and Br. Effluxes of these isotopes were measured mainly by the method of intracellular injection. Measurements of influxes were carried out under continuous intracellular perfusion with an isotonic solution of potassium sulfate. The Na permeability of the resting (excitable) axonal membrane was found to be roughly equal to the K permeability. The permeability to anion was far smaller than that to cations. It is emphasized that the axonal membrane has properties of a cation exchanger. The physicochemical nature of the "two stable states" of the excitable membrane is discussed on the basis of ion exchange isotherms. 相似文献
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