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Andrea Padoan Daniela Basso Carlo-Federico Zambon Tommaso Prayer-Galetti Giorgio Arrigoni Dania Bozzato Stefania Moz Filiberto Zattoni Rino Bellocco Mario Plebani 《Clinical proteomics》2018,15(1):23
Background
Lower urinary tract symptoms (LUTS) and prostate specific antigen-based parameters seem to have only a limited utility for the differential diagnosis of prostate cancer (PCa). MALDI-TOF/MS peptidomic profiling could be a useful diagnostic tool for biomarker discovery, although reproducibility issues have limited its applicability until now. The current study aimed to evaluate a new MALDI-TOF/MS candidate biomarker.Methods
Within- and between-subject variability of MALDI-TOF/MS-based peptidomic urine and serum analyses were evaluated in 20 and 15 healthy donors, respectively. Normalizations and approaches for accounting below limit of detection (LOD) values were utilized to enhance reproducibility, while Monte Carlo experiments were performed to verify whether measurement error can be dealt with LOD data. Post-prostatic massage urine and serum samples from 148 LUTS patients were analysed using MALDI-TOF/MS. Regression-calibration and simulation and extrapolation methods were used to derive the unbiased association between peptidomic features and PCa.Results
Although the median normalized peptidomic variability was 24.9%, the within- and between-subject variability showed that median normalization, LOD adjustment, and log2 data transformation were the best combination in terms of reliability; in measurement error conditions, intraclass correlation coefficient was a reliable estimate when the LOD/2 was substituted for below LOD values. In the patients studied, 43 peptides were shared by the urine and serum, and several features were found to be associated with PCa. Only few serum features, however, show statistical significance after the multiple testing procedures were completed. Two serum fragmentation patterns corresponded to the complement C4-A.Conclusions
MALDI-TOF/MS serum peptidome profiling was more efficacious with respect to post-prostatic massage urine analysis in discriminating PCa.4.
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The gentle separation mechanism has made field-flow fractionation particularly suited to samples of biotechnological interest, from proteins and nucleic acids to viruses, subcellular units and whole cells. Recent progress in field-flow fractionation technology, as well as the development of coupled techniques combining field-flow fractionation capabilities with the specificity and sensitivity of well-established analytical methods, opens up new biotechnological applications for field-flow fractionation. The most recent appealing applications include: sorting and fingerprinting of bacteria for whole-cell vaccine production; noninvasive and tagless sorting of immature and stem cells; separation of intact proteins and enzymes in top-down proteomics; and the development of flow-assisted, multianalyte immunoassays using nano- and micron-sized particles with immobilized biomolecules. 相似文献
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Randi T Garmo Steinar Waage Ståle Sviland Britt IF Henriksen Olav Østerås Olav Reksen 《Acta veterinaria Scandinavica》2010,52(1):11
Background
The objectives of this study were to investigate whether there were differences between Norwegian Red cows in conventional and organic farming with respect to reproductive performance, udder health, and antibiotic resistance in udder pathogens. 相似文献8.
Pramod Kumar Yadav Gurmit Singh Satendra Singh Budhayash Gautam Esmaiel IF Saad 《Bioinformation》2012,8(14):664-672
The emergence of multidrug-resistant strain of community-acquired methicillin resistant Staphylococcus aureus (CA-MRSA) strain
has highlighted the urgent need for the alternative and effective therapeutic approach to combat the menace of this nosocomial
pathogen. In the present work novel potential therapeutic drug targets have been identified through the metabolic pathways
analysis. All the gene products involved in different metabolic pathways of CA-MRSA in KEGG database were searched against
the proteome of Homo sapiens using the BLASTp program and the threshold of E-value was set to as 0.001. After database
searching, 152 putative targets were identified. Among all 152 putative targets, 39 genes encoding for putative targets were
identified as the essential genes from the DEG database which are indispensable for the survival of CA-MRSA. After extensive
literature review, 7 targets were identified as potential therapeutic drug target. These targets are Fructose-bisphosphate aldolase,
Phosphoglyceromutase, Purine nucleoside phosphorylase, Uridylate kinase, Tryptophan synthase subunit beta, Acetate kinase and
UDP-N-acetylglucosamine 1-carboxyvinyltransferase. Except Uridylate kinase all the identified targets were involved in more than
one metabolic pathways of CA-MRSA which underlines the importance of drug targets. These potential therapeutic drug targets
can be exploited for the discovery of novel inhibitors for CA-MRSA using the structure based drug design (SBDD) strategy. 相似文献
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Amanda M. Slomp Sandra M.W. Barreira Luise Z.B. Carrenho Camila C. Vandresen Ingrid F. Zattoni Stephanie M.S. Ló Juliana C.C. Dallagnol Diogo R.B. Ducatti Alexandre Orsato M. Eugênia R. Duarte Miguel D. Noseda Michel F. Otuki Alan G. Gonçalves 《Bioorganic & medicinal chemistry letters》2017,27(2):156-161
Sixteen porphyrins, including neutral, anionic and cationic meso-(aryl)porphyrins and meso-(1-methyl-4-pyridinium)porphyrins were herein evaluated in terms of their photosensitizing properties against HaCaT keratinocytes. After an initial screening, the cationic porphyrins were studied in more details, by both determining their log POW and performing PDT assays in lower porphyrin concentrations. Porphyrins presenting two or more adjacent positively charged groups, directly linked to the macrocycle meso positions, appeared to be the most effective photosensitizers. The present study also included the dicationic 5,10-diphenyl-15,20-di(1-methylpyridinium-4-yl)porphyrin (14b), which has previously shown promising results on a psoriasis-like in vivo model. Overall results indicated that the beneficial effect related to porphyrins on psoriasis can be related to the decreasing of keratinocyte viability. Furthermore, some of the cationic porphyrins studied appeared as candidates to be utilized as photosensitizers for psoriasis treatment. 相似文献
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Boccardo F Rubagotti A Battaglia M Zattoni F Bertaccini A Romagnoli A Conti G 《The International journal of biological markers》2006,21(2):123-126
BACKGROUND: There is growing evidence that IGF-1 and binding proteins may be involved in prostate cancer promotion and progression. PATIENTS AND METHODS: IGF-1 and binding proteins (IGFBP-1 and 3) serum levels were measured at baseline and after 3 and 6 months of treatment in a selected group of patients with prostate cancer who were randomly assigned to treatment with bicalutamide, bicalutamide plus anastrozole or bicalutamide plus tamoxifen in a comparative study investigating the role of pharmacological medication in the development of bicalutamide-induced gynecomastia. RESULTS: Bicalutamide monotherapy does not appear to alter the IGF-1/IGFBP system. In fact, the increase in IGF-1 levels induced by this treatment was paralleled by comparable increases in binding protein (IGFBP-3). No major changes from baseline up to month 6 either in IGF-1 or in IGFBP-1 and 3 were observed in the bicalutamide plus anastrozole arm. The addition of tamoxifen to bicalutamide produced a sharp decrease in IGF-1 levels (p<0.001) coupled with an increase in both IGFBP-1 (p=0.001) and, to a lesser extent, IGFBP-3 (p=0.5). CONCLUSIONS: The concurrent administration of tamoxifen and bicalutamide reduces the synthesis and bioavailability of IGF-1. Moreover, increased binding protein levels might exert antiproliferative and proapoptotic effects on prostate cancer cells, independently of the IGF-1/IGF receptor-mediated survival system. Both effects might have a synergistic inhibitory influence on prostate cancer growth. 相似文献