全文获取类型
收费全文 | 1408篇 |
免费 | 109篇 |
国内免费 | 1篇 |
专业分类
1518篇 |
出版年
2022年 | 8篇 |
2021年 | 22篇 |
2020年 | 8篇 |
2019年 | 12篇 |
2018年 | 14篇 |
2017年 | 15篇 |
2016年 | 21篇 |
2015年 | 31篇 |
2014年 | 45篇 |
2013年 | 73篇 |
2012年 | 87篇 |
2011年 | 85篇 |
2010年 | 54篇 |
2009年 | 52篇 |
2008年 | 83篇 |
2007年 | 86篇 |
2006年 | 82篇 |
2005年 | 75篇 |
2004年 | 71篇 |
2003年 | 66篇 |
2002年 | 75篇 |
2001年 | 25篇 |
2000年 | 26篇 |
1999年 | 17篇 |
1998年 | 19篇 |
1997年 | 15篇 |
1996年 | 11篇 |
1995年 | 10篇 |
1994年 | 18篇 |
1993年 | 10篇 |
1992年 | 16篇 |
1991年 | 12篇 |
1990年 | 22篇 |
1989年 | 16篇 |
1988年 | 23篇 |
1987年 | 19篇 |
1986年 | 26篇 |
1985年 | 14篇 |
1984年 | 23篇 |
1983年 | 10篇 |
1982年 | 15篇 |
1981年 | 11篇 |
1980年 | 11篇 |
1979年 | 10篇 |
1978年 | 7篇 |
1976年 | 7篇 |
1970年 | 7篇 |
1969年 | 6篇 |
1968年 | 11篇 |
1967年 | 7篇 |
排序方式: 共有1518条查询结果,搜索用时 0 毫秒
1.
2.
Interleukin 1 alpha mRNA in virus-transformed T and B cells 总被引:2,自引:0,他引:2
T Noma T Nakamura M Maeda M Okada Y Taniguchi Y Tagaya Y Yaoita J Yodoi T Honjo 《Biochemical and biophysical research communications》1986,139(1):353-360
IL-1 alpha cDNA clone was isolated from a T cell line infected by the human T lymphotropic retrovirus type-I (HTLV-I/ATLV). We found significant amounts of mRNA hybridizing to IL-1 alpha cDNA not only in HTLV-I-transformed T cells but also in Epstein-Barr Virus-transformed B cells. A part of IL-2 receptor inducing activity in Adult T cell leukemia (ATL) cell line seems to be due to IL-1 alpha. 相似文献
3.
S Honjo 《Journal of medical primatology》1985,14(2):75-89
The facilities and activities of the Japanese Primate Center at Tsukuba, Japan are described. The Center became partially functional in 1978 and was completed in 1979. The three main aims of the Primate Center are: to quarantine newly imported primate animals, to breed, and to study them. 相似文献
4.
Spontaneous malignant lymphoma in an African green monkey naturally infected with simian T-lymphotropic virus (STLV) 总被引:4,自引:0,他引:4
I Sakakibara Y Sugimoto A Sasagawa S Honjo H Tsujimoto H Nakamura M Hayami 《Journal of medical primatology》1986,15(5):311-318
An African green monkey naturally infected with simian T-lymphotropic virus (STLV) developed spontaneous malignant lymphoma of diffuse pleomorphic type. The clinical, hematological and histopathological characteristics were very similar to those of human adult T-cell leukemia. 相似文献
5.
Yasuko Kobayashi Candace J. S. Hassall Professor Geoffrey Burnstock 《Cell and tissue research》1986,244(3):605-612
Summary The present study describes the ultrastructure of non-neuronal cells and their interrelationships with intracardiac neurones present in cultures dissociated atria and interatrial septum from newborn guinea-pig. When compared with the in situ preparation, most of these features in culture were similar to those observed in situ, but some differences were also apparent. Both mature and immature Schwann cells were observed in culture, and as in situ, the latter were closely associated with intracardiac neurones, whilst the former were more widely separated. The ultrastructure of satellite cells was more variable in culture than in situ: three general types were distinguished on the basis of their 10-nm filament content. This variation could be due to conditions of culture. Interstitial cells were present in culture and closely resembled those described in situ, although there was less space between cultured interstitial cells and their associated cells. Many fibroblasts, some myoblasts and a few mast cells were also found in the culture preparations. 相似文献
6.
Molecular cloning and nucleotide sequencing of human immunoglobulin epsilon chain cDNA. 总被引:4,自引:1,他引:3 下载免费PDF全文
M Seno T Kurokawa Y Ono H Onda R Sasada K Igarashi M Kikuchi Y Sugino Y Nishida T Honjo 《Nucleic acids research》1983,11(3):719-726
DNA complementary to mRNA of human immunoglobulin E heavy chain (epsilon chain) isolated and purified from U266 cells has been synthesized and inserted into the PstI site of pBR322 by G-C tailing. This recombinant plasmid was used to transform E. coli chi 1776 to screen 1445 tetracycline resistant colonies. Nine clones (pGETI - 9) containing cDNA coding for the human epsilon chain were recognized by colony hybridization and Southern blotting analysis with a nick-translated human IgE genome fragment. The nucleotide sequence of the longest cDNA contained in pGET2 was determined. The results indicate that the sequence of 1657 nucleotides codes for 494 amino acids covering a part of the variable region and all of the constant region of the human epsilon chain. Most of the amino acid sequence deduced from the nucleotide sequence is in substantial agreement with that reported. Furthermore a termination codon after the -COOH terminal amino acid marks the beginning of a 3' untranslated region of 125 nucleotides with a poly A tail. Taking this into account, the structure of the human epsilon chain mRNA, except a part of the 5' end, is conserved fairly well in the cDNA insert in pGET2. 相似文献
7.
8.
Control of IL-2 receptor-alpha expression by IL-1, tumor necrosis factor, and IL-2. Complex regulation via elements in the 5' flanking region 总被引:2,自引:0,他引:2
G Plaetinck M C Combe P Corthésy P Sperisen H Kanamori T Honjo M Nabholz 《Journal of immunology (Baltimore, Md. : 1950)》1990,145(10):3340-3347
9.
Close linkage of MEN2A with RBP3 locus in Japanese kindreds 总被引:7,自引:0,他引:7
Masayuki Yamamoto Shin-ichiro Takai Tetsuro Miki Kazuyoshi Motomura Makoto Okazaki Isamu Nishisho Hideo Tateishi Akira Miyauchi Tasuku Honjo A. J. Pakstis Takesada Mori 《Human genetics》1989,81(3):287-288
Summary The gene responsible for multiple endocrine neoplasia type 2A (MEN2A) has recently been assigned to the pericentromeric region of chromsome 10 in European Caucasian kindreds by linkage analysis using a DNA marker, interstitial retinol-binding protein 3 (RBP3). We have found tight linkage between the MEN2A and RBP3 loci in Japanese MEN2A kindreds. The maximum lod score is 5.19 at a recombination fraction of 0.00. This result suggests that mutation of a certain gene close to RBP3 is responsible for MEN2A irrespective of ethnic backgrounds. 相似文献
10.
Circular DNA is excised by immunoglobulin class switch recombination 总被引:28,自引:0,他引:28
We have purified extrachromosomal circular DNAs from adult mouse spleen cells, and cloned into a phage vector the BamHl fragments hybridizing with C mu and S gamma 1 probes. We obtained 52 S mu+S gamma 1+ clones by screening 1.4 million phage clones derived from spleen cells stimulated with bacterial lipopolysaccharide and interleukin 4. We have identified the breakpoints of six clones that contain S gamma 1 and S mu sequences fused in the 5' to 3' orientation. All these switch recombination sites were assigned to the central repetitive sequences of the S mu and S gamma 1 regions. Since the common S mu-S gamma 1 sequences at the recombination sites are at most 2 bases long, typical homologous recombination cannot account for their joining. These findings provide direct evidence that mu-gamma 1 class switching can occur by the looping out and excision of chromosomal DNA, with formation of a circle. 相似文献