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1.
Zahra Amidzadeh Setayesh YasamiKhiabani Hamzeh Rahimi Shahin Bonakdar Davoud Shams Mahdi HabibiAnbouhi Majid Golkar Mohammad Ali Shokrgozar 《Journal of cellular and molecular medicine》2022,26(23):5929
Different growth factors can regulate stem cell differentiation. We used keratinocyte growth factor (KGF) to direct adipose‐derived stem cells (ASCs) differentiation into keratinocytes. To enhance KGF bioavailability, we targeted KGF for collagen by fusing it to collagen‐binding domain from Vibrio mimicus metalloprotease (vibrioCBD‐KGF). KGF and vibrioCBD‐KGF were expressed in Escherichia coli and purified to homogeneity. Both proteins displayed comparable activities in stimulating proliferation of HEK‐293 and MCF‐7 cells. vibrioCBD‐KGF demonstrated enhanced collagen‐binding affinity in immunofluorescence and ELISA. KGF and vibrioCBD‐KGF at different concentrations (2, 10, and 20 ng/ml) were applied for 21 days on ASCs cultured on collagen‐coated plates. Keratinocyte differentiation was assessed based on morphological changes, the expression of keratinocyte markers (Keratin‐10 and Involucrin), and stem cell markers (Collagen‐I and Vimentin) by real‐time PCR or immunofluorescence. Our results indicated that the expression of keratinocyte markers was substantially increased at all concentrations of vibrioCBD‐KGF, while it was observed for KGF only at 20 ng/ml. Immunofluorescence staining approved this finding. Moreover, down‐regulation of Collagen‐I, an indicator of differentiation commitment, was more significant in samples treated with vibrioCBD‐KGF. The present study showed that vibrioCBD‐KGF is more potent in inducing the ASCs differentiation into keratinocytes compared to KGF. Our results have important implications for effective skin regeneration using collagen‐based biomaterials. 相似文献
2.
Ramin Lotfi Alireza Rezaiemanesh Seyed Hamidreza Mortazavi Ali Gorgin Karaji Farhad Salari 《Journal of cellular physiology》2019,234(6):8579-8596
Asthma and allergic diseases are inflammatory conditions developed by excessive reaction of the immune system against normally harmless environmental substances. Although acute inflammation is necessary to eradicate the damaging agents, shifting to chronic inflammation can be potentially detrimental. Essential fatty-acids-derived immunoresolvents, namely, lipoxins, resolvins, protectins, and maresins, are anti-inflammatory compounds that are believed to have protective and beneficial effects in inflammatory disorders, including asthma and allergies. Accordingly, impaired biosynthesis and defective production of immunoresolvents could be involved in the development of chronic inflammation. In this review, recent evidence on the anti-inflam]matory effects of immunoresolvents, their enzymatic biosynthesis routes, as well as their receptors are discussed. 相似文献
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4.
Carl Bannerman Natalie Guzman Rachit Kumar Chelsea Nnebe Jordan Setayesh Amitej Venapally Jonathan H. Sussman 《Molecular biology of the cell》2020,31(24):2640
The importance of diversity is self-evident in medicine and medical research. Not only does diversity result in more impactful scientific work, but diverse teams of researchers and clinicians are necessary to address health disparities and improve the health of underserved communities. MD/PhD programs serve an important role in training physician-scientists, so it is critical to ensure that MD/PhD students represent diverse backgrounds and experiences. Groups who are underrepresented in medicine and the biomedical sciences include individuals from certain racial and ethnic backgrounds, individuals with disabilities, individuals from disadvantaged backgrounds, and women. However, underrepresented students are routinely discouraged from applying to MD/PhD programs due to a range of factors. These factors include the significant cost of applying, which can be prohibitive for many students, the paucity of diverse mentors who share common experiences, as well as applicants’ perceptions that there is inadequate support and inclusion from within MD/PhD programs. By providing advice to students who are underrepresented in medicine and describing steps programs can take to recruit and support minority applicants, we hope to encourage more students to consider the MD/PhD career path that will yield a more productive and equitable scientific and medical community. 相似文献
5.
Shirin Saberianpour Mohamad Hadi Saeed modaghegh Hamidreza Rahimi Mohammad Mahdi Kamyar 《Biophysical reviews》2021,13(1):139
Varicose veins are the most common vascular disease in humans. Veins have valves that help the blood return gradually to the heart without leaking blood. When these valves become weak, blood and fluid collect and pool by pressing against the walls of the veins, causing varicose veins. In the cardiovascular system, mechanical forces are important determinants of vascular homeostasis and pathological processes. Blood vessels are constantly exposed to a variety of hemodynamic forces, including shear stress and environmental strains caused by the blood flow. In varicose veins within the leg, venous blood pressure rises in the vein of the lower extremities due to prolonged standing, creating a peripheral tension in the vessel wall thereby causing mechanical stimulation of endothelial cells and vascular smooth muscle. Studies have shown that long-term increased exposure to vascular wall tension is associated with the overexpression of HIF-1α and HIF-2α and increased levels of MMP-2 and MMP-9, thereby reducing venous contraction and progressive venous dilatation, which is involved in the development of varicose veins. Following the expression of metalloproteinase, the expression of type 1 collagen increases, and the amount of type 3 collagen decreases. Therefore, collagen imbalance will cause the varicose veins to not stretch. Loss of structural proteins (type 3 collagen and elastin) in the vessel wall causes the loss of the biophysical properties of the varicose vein wall. This review article tries to elaborate on the effect of mechanical forces and sensors of these forces on the vascular wall in creating the mechanism of mechanosignaling, as well as the role of the onset of molecular signaling cascades in the pathology of varicose veins. 相似文献
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Different morphologies of Mucor hiemalis were induced and used for the production of ethanol and biomass from rice straw through a separate hydrolysis and fermentation process. The yield of enzymatic hydrolysis was improved from 40.4% for the untreated straw to 80–93% by employing sodium hydroxide and concentrated phosphoric acid pretreatments with or without ultrasonication. The best hydrolysis performance was achieved after pretreatment by sodium hydroxide assisted with ultrasonication. The ethanol yields from the hydrolysates were 0.39–0.44 g/g depending on the pretreatment method and the fungus morphology. The yeast‐like form of the fungus showed faster glucose assimilation and slightly higher ethanol yield compared to the other morphologies. The biomass yield of mostly yeast‐like cells was more than the other morphologies (0.202–0.282 g/g glucose). Moreover, the biomass of the yeast‐like cells had more protein content (46.7–52.4 %) compared to filamentous cells (37.7–46.3 %). The cell wall, alkali‐insoluble material (AIM) of the biomass, represented 16.3–20.1% of the biomass. On average, total chitin‐chitosan content of AIM of the biomass of purely filamentous, mostly filamentous, mostly yeast‐like, and purely yeast‐like forms of the fungus was 0.460, 0.373, 0.330, and 0.336 g/g AIM of the biomass, respectively. 相似文献
7.
Afrouz Farhad Paolo Rosa Ali Asghar Talebi Yaghoub Fathipour Hamidreza Hajiqanbar 《Journal of Asia》2019,22(4):1005-1012
The Iranian species in the Chrysis pulchella and C. varidens species groups are investigated. Six species are recognized, two of which are described for the first time: Chrysis gamberoonensis Farhad, Rosa and Talebi sp. nov. and Chrysis brunneamarginata Farhad, Rosa and Talebi sp. nov.. Chrysis gamberoonensis Farhad, Rosa and Talebi sp. nov. belongs to the C. pulchella group and is recognizable within this species group by its unique blue body coloration, scattered mesosoma punctures, polished pit row with elongated pits, and a small median tooth on the lateral edge of metasomal tergum 3. Chrysis brunneamarginata Farhad, Rosa and Talebi sp. nov. belongs to the C. varidens group and is recognizable by the usually colorless apical rim of metasomal tergum 3, and the unique shape of the anterior corners of the scutellum which are enlarged, thickened and directed backward covering the axillary trough. Dichotomous keys and distributional data for Iranian species included in these species groups are provided. Chrysis schwarzi Linsenmaier, 1968 is raised to species rank. The number of Iranian Chrysis species and subspecies is raised to 122.www.zoobank.org/urn:lsid:zoobank.org:pub:F419F860-3B90-4679-9A19-2CF5C255AE6B 相似文献
8.
Kamlesh Khunti Hamidreza Mani Felix Achana Nicola Cooper Laura J. Gray Melanie J. Davies 《PloS one》2015,10(9)
Background
Screening for type 2 diabetes (T2DM) and individuals at risk of diabetes has been advocated, yet information on the response rate and diagnostic yield of different screening strategies are lacking.Methods
Studies (from 1998 to March/2015) were identified through Medline, Embase and the Cochrane library and included if they used oral glucose tolerance test (OGTT) and WHO-1998 diagnostic criteria for screening in a community setting. Studies were one-step strategy if participants were invited directly for OGTT and two, three/four step if participants were screened at one or more levels prior to invitation to OGTT. The response rate and diagnostic yield were pooled using Bayesian random-effect meta-analyses.Findings
47 studies (422754 participants); 29 one-step, 11 two-step and seven three/four-step were identified. Pooled response rate (95% Credible Interval) for invitation to OGTT was 65.5% (53.7, 75.6), 63.1% (44.0, 76.8), and 85.4% (76.4, 93.3) in one, two and three/four-step studies respectively. T2DM yield was 6.6% (5.3, 7.8), 13.1% (4.3, 30.9) and 27.9% (8.6, 66.3) for one, two and three/four-step strategies respectively. The number needed to invite to the OGTT to detect one case of T2DM was 15, 7.6 and 3.6 in one, two, and three/four-step strategies. In two step strategies, there was no difference between the response or yield rates whether the first step was blood test or risk-score. There was evidence of substantial heterogeneity in rates across study populations but this was not explained by the method of invitation, study location (rural versus urban) and developmental index of the country in which the study was performed.Conclusions
Irrespective of the invitation method, developmental status of the countries and or rural/urban location, using a multi-step strategy increases the initial response rate to the invitation to screening for diabetes and reduces the number needed to have the final diagnostic test (OGTT in this study) for a definite diagnosis. 相似文献9.
Rocio Acuna-Hidalgo Denny Schanze Ariana Kariminejad Ann Nordgren Mohamad Hasan Kariminejad Peter Conner Giedre Grigelioniene Daniel Nilsson Magnus Nordenskjöld Anna Wedell Christoph Freyer Anna Wredenberg Dagmar Wieczorek Gabriele Gillessen-Kaesbach Hülya Kayserili Nursel Elcioglu Siavash Ghaderi-Sohi Payman Goodarzi Hamidreza Setayesh Maartje van de Vorst Marloes Steehouwer Rolph Pfundt Birgit Krabichler Cynthia Curry Malcolm G. MacKenzie Kym M. Boycott Christian Gilissen Andreas R. Janecke Alexander Hoischen Martin Zenker 《American journal of human genetics》2014
10.
Fasehee H Westers H Bolhuis A Antelmann H Hecker M Quax WJ Mirlohi AF van Dijl JM Ahmadian G 《Proteomics》2011,11(19):3905-3913
Sortases of Gram-positive bacteria catalyze the covalent C-terminal anchoring of proteins to the cell wall. Bacillus subtilis, a well-known host organism for protein production, contains two putative sortases named YhcS and YwpE. The present studies were aimed at investigating the possible sortase function of these proteins in B. subtilis. Proteomics analyses revealed that sortase-mutant cells released elevated levels of the putative sortase substrate YfkN into the culture medium upon phosphate starvation. The results indicate that YfkN required sortase activity of YhcS for retention in the cell wall. To analyze sortase function in more detail, we focused attention on the potential sortase substrate YhcR, which is co-expressed with the sortase YhcS. Our results showed that the sortase recognition and cell-wall-anchoring motif of YhcR is functional when fused to the Bacillus pumilus chitinase ChiS, a readily detectable reporter protein that is normally secreted. The ChiS fusion protein is displayed at the cell wall surface when YhcS is co-expressed. In the absence of YhcS, or when no cell-wall-anchoring motif is fused to ChiS, the ChiS accumulates predominately in the culture medium. Taken together, these novel findings show that B. subtilis has a functional sortase for anchoring proteins to the cell wall. 相似文献