全文获取类型
收费全文 | 1473篇 |
免费 | 131篇 |
出版年
2022年 | 8篇 |
2021年 | 15篇 |
2020年 | 11篇 |
2019年 | 22篇 |
2018年 | 30篇 |
2017年 | 19篇 |
2016年 | 32篇 |
2015年 | 50篇 |
2014年 | 65篇 |
2013年 | 88篇 |
2012年 | 118篇 |
2011年 | 88篇 |
2010年 | 59篇 |
2009年 | 60篇 |
2008年 | 91篇 |
2007年 | 84篇 |
2006年 | 76篇 |
2005年 | 56篇 |
2004年 | 55篇 |
2003年 | 67篇 |
2002年 | 50篇 |
2001年 | 33篇 |
2000年 | 30篇 |
1999年 | 28篇 |
1998年 | 13篇 |
1997年 | 11篇 |
1996年 | 9篇 |
1994年 | 8篇 |
1993年 | 7篇 |
1992年 | 14篇 |
1991年 | 15篇 |
1990年 | 17篇 |
1989年 | 11篇 |
1988年 | 19篇 |
1987年 | 16篇 |
1986年 | 9篇 |
1985年 | 19篇 |
1984年 | 11篇 |
1983年 | 14篇 |
1982年 | 10篇 |
1981年 | 14篇 |
1980年 | 9篇 |
1979年 | 15篇 |
1977年 | 8篇 |
1976年 | 13篇 |
1974年 | 9篇 |
1973年 | 11篇 |
1970年 | 12篇 |
1969年 | 7篇 |
1967年 | 7篇 |
排序方式: 共有1604条查询结果,搜索用时 62 毫秒
1.
2.
K Scheuch W D Pietruschka E Hentschel P Winiecki G Gruber 《Activitas nervosa superior》1988,30(3):169-173
Biochemical and immunological parameters, physical and mental performance, subjective complaints and behavioural characteristics were compared before and after 14 final examinations undertaken by 64 students during a three-month examination period. A decrease in cholesterol, triglycerides, HDL-cholesterol, physical performance and an increase in LDL/HDL cholesterol quotient, lactate level, mental performance were accompanied by a lower frequency of mental complaints and higher frequency of physical complaints. From the multidimensional variance and discriminant analysis 17 of the 44 variables discriminated between the state prior to and after the examination period. The results are interpreted in terms of the psychophysiological adaptation to adequate mental stress. 相似文献
3.
James J. Krupa Kevin R. Hopper Samuel B. Gruber Jason M. Schmidt James D. Harwood 《Ecology and evolution》2020,10(11):4762-4772
- Plant–animal interactions are diverse and widespread shaping ecology, evolution, and biodiversity of most ecological communities. Carnivorous plants are unusual in that they can be simultaneously engaged with animals in multiple mutualistic and antagonistic interactions including reversed plant–animal interactions where they are the predator. Competition with animals is a potential antagonistic plant–animal interaction unique to carnivorous plants when they and animal predators consume the same prey.
- The goal of this field study was to test the hypothesis that under natural conditions, sundews and spiders are predators consuming the same prey thus creating an environment where interkingdom competition can occur.
- Over 12 months, we collected data on 15 dates in the only protected Highland Rim Wet Meadow Ecosystem in Kentucky where sundews, sheet‐web spiders, and ground‐running spiders co‐exist. One each sampling day, we attempted to locate fifteen sites with: (a) both sheet‐web spiders and sundews; (b) sundews only; and (c) where neither occurred. Sticky traps were set at each of these sites to determine prey (springtails) activity–density. Ground‐running spiders were collected on sampling days. DNA extraction was performed on all spiders to determine which individuals had eaten springtails and comparing this to the density of sundews where the spiders were captured.
- Sundews and spiders consumed springtails. Springtail activity–densities were lower, the higher the density of sundews. Both sheet‐web and ground‐running spiders were found less often where sundew densities were high. Sheet‐web size was smaller where sundew densities were high.
- The results of this study suggest that asymmetrical exploitative competition occurs between sundews and spiders. Sundews appear to have a greater negative impact on spiders, where spiders probably have little impact on sundews. In this example of interkingdom competition where the asymmetry should be most extreme, amensalism where one competitor experiences no cost of interaction may be occurring.
4.
5.
Strategies for signal amplification in nucleic acid detection 总被引:3,自引:0,他引:3
S. Calin Andras J. Brian Power Edward C. Cocking Michael R. Davey 《Molecular biotechnology》2001,19(1):29-44
Many aspects of molecular genetics necessitate the detection of nucleic acid sequences. Current approaches involving target
amplification (in situ PCR, Primed in situ Labeling, Self-Sustained Sequence Replication, Strand Displacement Amplification), probe amplification (Ligase Chain Reaction,
Padlock Probes, Rolling Circle Amplification) and signal amplification (Tyramide Signal Amplification, Branched DNA Amplification)
are summarized in the present review, together with their advantages and limitations. 相似文献
6.
7.
Manfred Keller Andras Seregi Georg Hertting Rolf Jackisch 《Neurochemistry international》1987,10(4):433-443
Prostaglandin (PG) and thromboxane B2 (TXB2) biosynthesis was studied in cultured astrocytes from neonatal rat brain hemispheres. After two weeks of cultivation, prostanoids were formed with the spectrum: PGD2 > TXB2 > PGF2 > PGE2, as measured by specific radioimmunoassays. Under basal conditions PGD2 biosynthesis (9.55 ng/mg protein/15 min) was in the same order of magnitude as the sum of the other prostanoids. The formation of prostanoids was stimulated in a concentration dependent manner (up to 6–10 fold) by the calcium ionophore A 23187 (0.01–10 μM) as well as by melittin (0.01–5 μg/ml), phospholipase A2 (10–40 U/ml) and phospholipase C (0.01–1 U/ml). Basal and evoked PG and TXB2 biosynthesis depended on the availability of Ca2+, as demonstrated in Ca2+ free incubation medium containing Na2EDTA (1 μM), or with verapamil (100 μM) and 3,4,5-trimethoxybenzoic acid-8-(diethylamino)-octylester-HCl (TMB-8, 1–100 μM). Indomethacin (10 μM), mepacrine (100 μM) and p-bromophenacylbromide (50 μ M) inhibited basal and evoked PG formation. Thin-layer chromatography (TLC) detection after incubation of the cells with [3H]arachidonic acid (1 μCi/ml, for 60 min) confirmed the results obtained by radioimmunoassay. Incubation of [3H]arachidonic acid labelled cells with inonophore or phospholipases, followed by lipid extraction and TLC, showed that A 23187 liberated [3H]arachidonic acid predominantly from phosphatidylethanolamine, whereas phospholipase A2 and C reduced mainly the labelling of the phosphatidyl-inositol/-choline fraction. Potassium depolarization of the cells did not enhance prostanoid formation. Similarly, drugs with affinity to - or β-adrenoceptors, or to dopamine-, 5-hydroxytryptamine-, muscarine-, histamine-, glutamate-, aspartate-, GABA, adenosine- and opioid-receptors failed to stimulate prostanoid biosynthesis. Also compounds like angiotensin, bradykinin and thrombin were ineffective in this respect.
In conclusion, our results confirm that cultured astrocytes possess the complete pattern of enzymes necessary for prostanoid formation and hence might play a crucial role in brain prostanoid biosynthesis. Stimulation of prostanoid biosynthesis involves Ca2+-dependent activation of phospholipase A2, cyclooxygenase reaction and further PG metabolism. However, the endogenous stimulus for enhanced prostanoid synthesis in the brain still has to be established. 相似文献
8.
B. Mayr K. Gruber 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1987,73(6):832-835
Summary Ag-NOR staining and a counterstain enhanced fluorescence technique (chromomycin A3/distamycin A/DAPI-staining = CDD-method) and G-banding, respectively, have been applied to the zebu (Bos indicus L.) chromosomes. The nucleolus organizer regions (NORs) were found in the telomeric regions of chromosomes nos. 2, 3, 4, 11, and 28. CDD staining led to a well-defined R-banding pattern along the chromosome arms and to the visualization of centric heterochromatic bands of variable sizes. 相似文献
9.
The evolution of egg yolk proteins 总被引:13,自引:0,他引:13
10.
Experimentally produced monozygotic twins, natural opposite sex blood chimeras (freemartins), and several pedigrees were used to evaluate the genetic influences on the nucleolus organizer region (NOR) patterns in cattle. In monozygotic twins, the NOR patterns of both twins are extremely similar. In chimeras, NOR patterns of genetically identical, peripheral blood lymphocytes (PBL) from the two partners resemble each other. In contrast, genetically different PBL (sib organ) differ significantly in the same environment. A high heritability of the individual NOR patterns is also demonstrated in our 23 pedigrees. In conclusion, our data demonstrate that variation in NOR expression is predominantly due to genetic factors. 相似文献