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1.
2.
Characterization and cDNA cloning of phospholipase C-gamma, a major substrate for heparin-binding growth factor 1 (acidic fibroblast growth factor)-activated tyrosine kinase. 总被引:27,自引:5,他引:22
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W H Burgess C A Dionne J Kaplow R Mudd R Friesel A Zilberstein J Schlessinger M Jaye 《Molecular and cellular biology》1990,10(9):4770-4777
Heparin-binding growth factors (HBGFs) bind to high-affinity cell surface receptors which possess intrinsic tyrosine kinase activity. A Mr 150,000 protein phosphorylated on tyrosine in response to class 1 HBGF (HBGF-1) was purified and partially sequenced. On the basis of this sequence, cDNA clones were isolated from a human endothelial cell library and identified as encoding phospholipase C-gamma. Phosphorylation of phospholipase C-gamma in intact cells treated with HBGF-1 was directly demonstrated by using antiphospholipase C-gamma antibodies. Thus, HBGF-1 joins epidermal growth factor and platelet-derived growth factor, whose receptor activation leads to tyrosine phosphorylation and probable activation of phospholipase C-gamma. 相似文献
3.
Salas-Prato Milagros Tanguay Jean-Francois Lefebvre Yves Wojciechowicz Don Liem H. Heng Barnes David W. Ouellette Ginette Muller-Eberhard Ursula 《In vitro cellular & developmental biology. Plant》1988,24(5):470-470
In Vitro Cellular &; Developmental Biology - Plant - 相似文献
4.
F Bellot G Crumley J M Kaplow J Schlessinger M Jaye C A Dionne 《The EMBO journal》1991,10(10):2849-2854
Recent evidence shows that different fibroblast growth factors (FGF) bind with similar high affinities to two FGF receptors (FGFR) called flg and bek. In order to explore the mechanism of FGFR tyrosine autophosphorylation, we have generated cell lines which co-express a kinase-negative mutant of FGFR and an active form of FGFR. The following transfected NIH 3T3 cells were generated: (i) cells which express a shorter truncated form of bek (two Ig domains) together with a kinase-negative mutant of full length bek (bek K517A), (ii) cells which express wild-type bek together with kinase-negative flg (flg K514A) and (iii) cells co-expressing wild-type flg together with bek K517A. Immunoprecipitations with either bek-or flg-specific antisera followed by immunoblotting indicated that the double transfectants express the desired receptor species. The addition of acidic FGF (aFGF) to the various cell lines followed by immunoprecipitation with anti-FGFR antibodies and immunoblotting with anti-phosphotyrosine specific antibodies indicated that aFGF induces tyrosine phosphorylation of the kinase-negative FGFR mutants. These results show that tyrosine autophosphorylation of the kinase-negative FGFR is mediated by a transphosphorylation mechanism and that both homologous (bek----bek) and heterologous (bek----flg and flg----bek) transphosphorylation occurs in living cells. Recent evidence shows that tyrosine autophosphorylation of receptors with tyrosine kinase activities is essential for mediating interactions with signaling molecules. Therefore, heterologous transphosphorylation could amplify the response of cells to various forms of FGFs and their cognate receptors. 相似文献
5.
6.
Summary Newborn rat adipocyte precursors, isolated from inguinal fat pads of 2 day-old NBR rats proliferate and undergo adipose differentiation
in defined medium in the absence of serum when cultivated on polylysine coated dishes in DME-F12 medium supplemented with
fibronectin, insulin, transferrin and FGF. After 7 days in culture in these conditions, 90% of the cells have undergone differentiation
as measured by the increase of G3PDH specific activity and by the accumulation of triglycerides in their cytoplasm. In contrast,
the cells cultivated in the presence of 10% fetal bovine serum, have a limited ability to differentiate. These results indicate
that newborn rat adipocyte precursors from inguinal fat pads do not require the presence of an undefined adipogenic factor
in order to differentiate in culture. In contrast, proliferation and differentiation are dependent on the presence of insulin
in the culture medium. Moreover, the data presented in this paper show that the rat adipocyte precursor culture represents
a rapid and reproducible system for investigating the processes of adipose tissue development and for studying the negative
and positive regulators of the adipose differentiation in a controlled environment.
This work was supported by grants from the Juvenile Diabetes Foundation, File #185221 and from the National Institutes of
Health 1 PO1 CA37589.
Editor’s Statement This paper extends to primary cultures the serum-free methods previously applied to studies of adipocyte
differentiation in established lines. The observation that serum can block differentiation in this system suggests the existence
of previously unrecognized circulating plasma or platelet factors affecting adipocyte differentiation, and the model developed
provides an assay for the identification of these factors. 相似文献
7.
Lavoie J. M.; Dionne N.; Helie R.; Brisson G. R. 《Journal of applied physiology》1987,62(3):1084-1089
The purpose of this investigation was to evaluate the effects of 24-h carbohydrate-poor diet on metabolic and hormonal responses induced by prolonged exercise in both follicular (FP) and luteal (LP) phases of the menstrual cycle. At mid-FP and at mid-LP, seven eumenorrheic young women [means +/- SE; chronological age, 21.1 +/- 0.6 yr; O2 uptake (VO2) peak, 43.7 +/- 2.0 ml X kg-1 X min-1; body fat, 19.2 +/- 2.0%] were subjected to a 90-min bicycle exercise period at an intensity representing 63% of their measured VO2 peak. Venous blood samples obtained before and during exercise were analyzed for levels of substrates (glucose, lactate, free fatty acids, glycerol) and hormones (luteinizing hormone, progesterone, estradiol, insulin, glucagon, cortisol, catecholamines). Contrary to FP, a significant (P less than 0.01) decrease in blood glucose concentration was observed after 70 and 90 min of exercise during LP. Significant phase differences were also observed for blood lactate (highest in FP), cortisol (highest in LP), and progesterone (highest in LP). Although not significantly different, tendencies for menstrual phase dissociations were noticed for some of the other measured variables. Hence, a menstrual phase dissociation in circulating glucose level, unmasked by a prolonged exercise performed after a 24-h carbohydrate-poor diet, suggests to the authors a specific metabolic involvement for gonadotrophic and/or gonadal hormones. 相似文献
8.
Ginette Pauly Abdelhamid Yani Louis Piovetti Colette Bernard-Dagan 《Phytochemistry》1983,22(4):957-959
The essential oils of the leaves of Cupressus dupreziana and Cupressus sempervirens were compared. The composition of the hydrocarbon fraction showed a great similarity between the two species. 相似文献
9.
Suzanne A. Adjoa Claude Bouchard Pieter Coetzer Tim D. Noakes France T. Dionne 《Human genetics》1994,93(3):347-348
A DNA polymorphism of the nuclear-encoded subunit Va of the human cytochrome c oxidase (COX), a mitochondrial respiratory enzyme, is reported. No polymorphism was detected in genes for the subunits IV and Vb of the same enzyme. 相似文献
10.
Two alleles of 10.1 and 8.1 kb of the human glycogen synthase gene have been revealed with the restriction enzyme EcoRI. 相似文献