The adjuvant effect of selenium nanoparticles,Triton X-114 detergent micelles,and lecithin liposomes for <Emphasis Type="Italic">Escherichia coli</Emphasis> antigens

We studied the adjuvant properties of micelles from nonionogenic detergents, liposome, and selenium nanoparticles containing extracellular and intracellular vaccine antigens of a weakly virulent α-hemolytic Escherichia coli B-5 strain used for the immunization of experimental animals. Triton X-100 was used as a nonionogenic detergent for micelle preparation. The liposomes were obtained on the basis of lecithin from a chicken egg and E. coli B-5 membrane lipids. Native lipoproteins of E. coli B-5 cells and peptides for the proteolytic hydrolysis of toxin-containing culture liquid were used as antigens for micelles and liposomes. The obtained data suggested that micelles, liposomes, and selenium nanoparticles can be used for immunization with cellular and extracellular E. coli antigens.     

Phage Antibodies as Bioreceptors for Ampicillin Detection

Applied Biochemistry and Microbiology - Ampicillin-specific antibodies were obtained using a sheep display library of scFv fragments (Griffin.1, UK). Ampicillin was determined by dot-immunoassay...