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Wei-Hsin Chen Ching-Liang Hsieh Chun-Ping Huang Tzu-Jou Lin Jason TC Tzen Tin-Yun Ho Yi-Wen Lin 《Journal of biomedical science》2011,18(1):82
Background
Peripheral tissue inflammation initiates hyperalgesia accompanied by tissue acidosis, nociceptor activation, and inflammation mediators. Recent studies have suggested a significantly increased expression of acid-sensing ion channel 3 (ASIC3) in both carrageenan- and complete Freund's adjuvant (CFA)-induced inflammation. This study tested the hypothesis that acupuncture is curative for mechanical hyperalgesia induced by peripheral inflammation. 相似文献3.
Afjehi-Sadat L Shin JH Felizardo M Lee K Slavc I Lubec G 《Biochimica et biophysica acta》2005,1747(1):67-80
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Background
Owing to rapid expansion of protein structure databases in recent years, methods of structure comparison are becoming increasingly effective and important in revealing novel information on functional properties of proteins and their roles in the grand scheme of evolutionary biology. Currently, the structural similarity between two proteins is measured by the root-mean-square-deviation (RMSD) in their best-superimposed atomic coordinates. RMSD is the golden rule of measuring structural similarity when the structures are nearly identical; it, however, fails to detect the higher order topological similarities in proteins evolved into different shapes. We propose new algorithms for extracting geometrical invariants of proteins that can be effectively used to identify homologous protein structures or topologies in order to quantify both close and remote structural similarities. 相似文献5.
Fuhai Li Xiaobo Zhou Wanting Huang Chung-Che Chang Stephen TC Wong 《BMC bioinformatics》2010,11(1):200
Background
DNA copy number aberration (CNA) is very important in the pathogenesis of tumors and other diseases. For example, CNAs may result in suppression of anti-oncogenes and activation of oncogenes, which would cause certain types of cancers. High density single nucleotide polymorphism (SNP) array data is widely used for the CNA detection. However, it is nontrivial to detect the CNA automatically because the signals obtained from high density SNP arrays often have low signal-to-noise ratio (SNR), which might be caused by whole genome amplification, mixtures of normal and tumor cells, experimental noise or other technical limitations. With the reduction in SNR, many false CNA regions are often detected and the true CNA regions are missed. Thus, more sophisticated statistical models are needed to make the CNAs detection, using the low SNR signals, more robust and reliable. 相似文献6.
E. E. Hernández Cuadrado L. D. Solis Murgas V. de Oliveira Felizardo M. Rodrigues Ferreira E. de Souza Andrade 《Zeitschrift fur angewandte Ichthyologie》2014,30(2):329-333
The objective of this study was to evaluate the effects of six activating solutions on duration of sperm motility, fertilization rate (FR), and hatching rate (HR) of Prochilodus lineatus (Valenciennes, 1837). The activating solutions (SA) used were: SA0 (199 mOsm kg?1, pH 8.5), SA1 (138 mOsm kg?1, pH 7.5), SA2 (256 mOsm kg?1, pH 7.5), SA3 (131 mOsm kg?1, pH 10), NaCl (92 mOsm kg?1, pH 7.5) and distilled water (32 mOsm kg?1, pH 7.5). SA1 induced the highest motility, FR and HR, compared with the other activating solutions. The lowest motility was obtained with SA0, with no fertilization or hatching, whereas motility was zero with SA2 and SA3. It is possible to conclude that the solution SA1 can be used for the activation of gametes during fertilization in induced reproduction of curimba to achieve higher fertilization and hatching rates. Thus, it was found that the osmolality and pH of activating solutions, probably with the participation of dissolved substances therein, are the main factors acting on semen motility after activation. 相似文献
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Rafael Luiz Pereira Raphael José Ferreira Felizardo Marcos Ant?nio Cenedeze Meire Ioshie Hiyane ênio José Bassi Mariane Tami Amano Clarice Sylvia Taemi Origassa Reinaldo Correia Silva Cristhiane Fávero Aguiar Sylvia Mendes Carneiro Jo?o Bosco Pesquero Ronaldo Carvalho Araújo Alexandre de Castro Keller Renato C. Monteiro Ivan Cruz Moura Alvaro Pacheco-Silva Niels Olsen Saraiva Camara 《Disease models & mechanisms》2014,7(6):701-710
Focal and segmental glomerulosclerosis (FSGS) is one of the most important renal diseases related to end-stage renal failure. Bradykinin has been implicated in the pathogenesis of renal inflammation, whereas the role of its receptor 2 (B2RBK; also known as BDKRB2) in FSGS has not been studied. FSGS was induced in wild-type and B2RBK-knockout mice by a single intravenous injection of Adriamycin (ADM). In order to further modulate the kinin receptors, the animals were also treated with the B2RBK antagonist HOE-140 and the B1RBK antagonist DALBK. Here, we show that the blockage of B2RBK with HOE-140 protects mice from the development of FSGS, including podocyte foot process effacement and the re-establishment of slit-diaphragm-related proteins. However, B2RBK-knockout mice were not protected from FSGS. These opposite results were due to B1RBK expression. B1RBK was upregulated after the injection of ADM and this upregulation was exacerbated in B2RBK-knockout animals. Furthermore, treatment with HOE-140 downregulated the B1RBK receptor. The blockage of B1RBK in B2RBK-knockout animals promoted FSGS regression, with a less-inflammatory phenotype. These results indicate a deleterious role of both kinin receptors in an FSGS model and suggest a possible cross-talk between them in the progression of disease.KEY WORDS: Focal and segmental glomerulosclerosis, Bradykinin receptors, Inflammation, Podocyte, Fibrosis 相似文献
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Aberrant expression of cytoskeleton proteins in hippocampus from patients with mesial temporal lobe epilepsy 总被引:8,自引:0,他引:8
Summary. Mesial temporal lobe epilepsy (MTLE), the most common form of epilepsy, is characterised by cytoarchitectural abnormalities
including neuronal cell loss and reactive gliosis in hippocampus. Determination of aberrant cytoskeleton protein expression
by proteomics techniques may help to understand pathomechanism that is still elusive. We searched for differential expression
of hippocampal proteins by an analytical method based on two-dimensional gel electrophoresis (2-DE) coupled with mass spectrometry
unambiguously identifying 77 proteins analysed in eight control and eight MTLE hippocampi. Proteins were quantified and we
observed 18 proteins that were altered in MTLE. Cytoskeleton proteins tubulin α-1 chain, β-tubulin, profilin II, neuronal
tropomodulin were significantly reduced and one actin spot was missing, whereas ezrin and vinculin were significantly increased
in MTLE. Proteins of several classes as e.g. antioxidant proteins (peroxiredoxins 3 and 6), chaperons (T-complex protein 1-α,
stress-induced-phosphoprotein 1), signaling protein MAP kinase kinase 1, synaptosomal proteins (synaptotagmin I, α-synuclein),
NAD-dependent deacetylase sirtuin-2 and 26S protease regulatory subunit 7 protein, neuronal-specific septin 3 were altered
in MTLE. Taken together, the findings may represent or lead to cytoskeletal impairment; aberrant antioxidant proteins, chaperons,
MAP kinase kinase 1 and NAD-dependent deacetylase sirtuin-2 may have been involved in pathogenetic mechanisms and altered
synaptosomal protein expression possibly reflects synaptic impairment in MTLE.
J. W. Yang and T. Czech have equally contributed to the paper. 相似文献
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