全文获取类型
收费全文 | 2162篇 |
免费 | 146篇 |
出版年
2024年 | 2篇 |
2023年 | 12篇 |
2022年 | 29篇 |
2021年 | 75篇 |
2020年 | 38篇 |
2019年 | 45篇 |
2018年 | 60篇 |
2017年 | 47篇 |
2016年 | 67篇 |
2015年 | 116篇 |
2014年 | 114篇 |
2013年 | 164篇 |
2012年 | 206篇 |
2011年 | 217篇 |
2010年 | 115篇 |
2009年 | 107篇 |
2008年 | 141篇 |
2007年 | 117篇 |
2006年 | 97篇 |
2005年 | 96篇 |
2004年 | 95篇 |
2003年 | 74篇 |
2002年 | 61篇 |
2001年 | 24篇 |
2000年 | 22篇 |
1999年 | 15篇 |
1998年 | 14篇 |
1997年 | 6篇 |
1996年 | 8篇 |
1995年 | 12篇 |
1994年 | 2篇 |
1993年 | 6篇 |
1992年 | 12篇 |
1991年 | 9篇 |
1990年 | 12篇 |
1989年 | 12篇 |
1988年 | 5篇 |
1987年 | 5篇 |
1986年 | 9篇 |
1985年 | 5篇 |
1984年 | 7篇 |
1983年 | 2篇 |
1978年 | 6篇 |
1977年 | 4篇 |
1976年 | 2篇 |
1974年 | 3篇 |
1973年 | 5篇 |
1971年 | 1篇 |
1965年 | 1篇 |
1907年 | 1篇 |
排序方式: 共有2308条查询结果,搜索用时 15 毫秒
1.
2.
3.
Energy metabolism and lipid peroxidation of human erythrocytes as a function of increased oxidative stress. 总被引:5,自引:0,他引:5
B Tavazzi D Di Pierro A M Amorini G Fazzina M Tuttobene B Giardina G Lazzarino 《European journal of biochemistry》2000,267(3):684-689
To study the influence of oxidative stress on energy metabolism and lipid peroxidation in erythrocytes, cells were incubated with increasing concentrations (0.5-10 mM) of hydrogen peroxide for 1 h at 37 degrees C and the main substances of energy metabolism (ATP, AMP, GTP and IMP) and one index of lipid peroxidation (malondialdehyde) were determined by HPLC on cell extracts. Using the same incubation conditions, the activity of AMP-deaminase was also determined. Under nonhaemolysing conditions (at up to 4 mM H2O2), oxidative stress produced, starting from 1 mM H2O2, progressive ATP depletion and a net decrease in the intracellular sum of adenine nucleotides (ATP + ADP + AMP), which were not paralleled by AMP formation. Concomitantly, the IMP level increased by up to 20-fold with respect to the value determined in control erythrocytes, when cells were challenged with the highest nonhaemolysing H2O2 concentration (4 mM). Efflux of inosine, hypoxanthine, xanthine and uric acid towards the extracellular medium was observed. The metabolic imbalance of erythrocytes following oxidative stress was due to a dramatic and unexpected activation of AMP-deaminase (a twofold increase of activity with respect to controls) that was already evident at the lowest dose of H2O2 used; this enzymatic activity increased with increasing H2O2 in the medium, and reached its maximum at 4 mM H2O2-treated erythrocytes (10-fold higher activity than controls). Generation of malondialdehyde was strictly related to the dose of H2O2, being detectable at the lowest H2O2 concentration and increasing without appreciable haemolysis up to 4 mM H2O2. Besides demonstrating a close relationship between lipid peroxidation and haemolysis, these data suggest that glycolytic enzymes are moderately affected by oxygen radical action and strongly indicate, in the change of AMP-deaminase activity, a highly sensitive enzymatic site responsible for a profound modification of erythrocyte energy metabolism during oxidative stress. 相似文献
4.
It has long been debated whether the mind consists of specialized and independently evolving modules, or whether and to what extent a general factor accounts for the variance in performance across different cognitive domains. In this study, we used a hierarchical Bayesian model to re-analyse individual level data collected on seven primate species (chimpanzees, bonobos, orangutans, gorillas, spider monkeys, brown capuchin monkeys and long-tailed macaques) across 17 tasks within four domains (inhibition, memory, transposition and support). Our modelling approach evidenced the existence of both a domain-specific factor and a species factor, each accounting for the same amount (17%) of the observed variance. In contrast, inter-individual differences played a minimal role. These results support the hypothesis that the mind of primates is (at least partially) modular, with domain-specific cognitive skills undergoing different evolutionary pressures in different species in response to specific ecological and social demands. 相似文献
5.
6.
A Bellelli M Brunori S G Condò B Giardina 《The Journal of biological chemistry》1987,262(6):2624-2629
The properties of human hemoglobin reacted with 2-nor-2-formylpyridoxal 5'-phosphate, a bifunctional derivative of pyridoxal 5'-phosphate, have been investigated both from an equilibrium and kinetic point of view. The experimental data, interpreted in terms of the two-state allosteric model, indicate that a perturbed R state is characteristic of this modified low ligand affinity hemoglobin. In flash photolysis experiments, a quickly reacting component is always observed, in spite of the lack of dissociation into free dimers; this kinetic behavior is thought to reflect the presence of functionally independent alpha beta dimers, still connected by the flexible cross-link but forming an open hemoglobin tetramer. Two possible models for the interpretation of the kinetics of CO and/or haptoglobin binding are presented and discussed. 相似文献
7.
G De Sanctis G Falcioni B Giardina F Ascoli M Brunori 《Journal of molecular biology》1986,188(1):73-76
A domain of 108 amino acid residues (32 to 139), obtained by digestion of horse heart apomyoglobin with clostripain, was found to bind protoheme in a 1 to 1 molar ratio. This domain is 33 amino acid residues larger than the protein segment encoded by the central exon in seal myoglobin. Flash photolysis experiments have shown that reconstituted "mini-myoglobin" is very similar to myoglobin in the combination reaction with carbon monoxide and with oxygen, and in the oxygen replacement reaction by carbon monoxide. These experiments provide for the first time direct evidence for the presence of a structural and functional domain, closely corresponding to the segment encoded by the central exon of the myoglobin gene, which contains the information for binding the natural heme and for maintaining the native folding typical of a respiratory protein. 相似文献
8.
Sergio Sgorbati Marisa Levi Elio Sparvoli Federica Trezzi Giovanni Lucchini 《Physiologia plantarum》1986,68(3):471-476
Cytometry and flow cytometry were used to study characteristics of fluorescence of the DNA-DAPI complex in nuclei released from different fresh and formaldehyde-fixed pea ( Pisum sativum L. cv. Lincoln) tissues. The two methods of isolation are compared and discussed as well as their possible use for quantitative analysis of DNA in plant tissues. With fixed tissues it is possible to obtain a number of nuclei sufficient for the flow cytometric analysis, even using small amounts of plant tissue. 相似文献
9.
10.
Ole Brix Astrid Bårdgard Svein Mathisen Nicholas Tyler Matti Nuutinen Sarveriò G. Condo Bruno Giardina 《Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology》1990,159(6):655-660
Summary The oxygen binding of whole blood from humans and two arctic mammals, reindeer and muskox, has been studied as a function of carbon dioxide and temperature. All bloods display a marked Bohr effect with Bohr coefficients in the range –0.44––0.73. The Bohr effect is more pronounced at 20°C. The temperature sensitivity of reindeer and muskox blood expressed by the apparent heat of oxygenation, H, is almost three times lower than that of human HbA under the same experimental conditions. This thermodynamic difference gives special benefits to arctic mammals with large heterothermy by safeguarding oxygen unloading at very low ambient temperatures. 相似文献