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排序方式: 共有105条查询结果,搜索用时 31 毫秒
1.
To assess the dynamics of synthesis of the wall by regenerating Candida albicans protoplasts deposition of chitin and mannoproteins were investigated ultrastructurally using wheat germ agglutinin conjugated with either horseradish peroxidase or colloidal gold, and Concanavalin A coupled to ferritin respectively.Freshly prepared protoplasts lacked wheat germ agglutinin receptor sites but after 1–2 h of regeneration, they were detected. After 4–5 h of regeneration, the cell wall showed a discrete structure which was only labelled with wheat germ agglutinin in thin sections. At this stage of regeneration the outermost layer of the wall was labelled with clusters of Concanavalin A-ferritin particles.After 8 h regeneration, the cell wall appeared compact, and homogenously marked with wheat germ agglutinin whereas only the surface layers appeared consistently labelled with Concanavalin A-ferritin.From these observations we conclude that C. albicans protoplasts are able to regenerate in liquid medium a cell wall consisting of a network of chitin fibrils and mannoproteins at least (glucan polymers were not determined in the present cytological study). The former are the fundamental component of the inner layers at early stages of regeneration, whereas the latter molecules are predominant in the outer layers of the wall.Abbreviations WGA-HRP
wheat germ agglutinin conjugated with horseradish peroxidase
- WGA-Au
wheat germ agglutinin conjugated with colloidal gold
- Con A-ferritin
Concanavalin A coupled to ferritin 相似文献
2.
Hortensia Rico Enrique Herrero Fernando Miragall Rafael Sentandreu 《Archives of microbiology》1991,156(2):111-114
Depending upon growth temperature, Candida albicans can exhibit two different morphologies, a budding yeast or a mycelium. By studying the distribution of concanavalin A-ferritin particles on the cell wall surface during bud and germ tube formation, we have elucidated the way cell wall extension occurs. Both processes initially require the localized lysis of the wall in order to allow the incorporation of the newly synthesized material. Later on, the cell wall behaves as an elastic structure, allowing extension by an intosusception process and, as a consequence, cell growth.Abbreviation Con A
concanavalin A 相似文献
3.
Maria Concepción Escorial Hortensia Sixto José-Maria García-Baudin Mariá-Cristina Chueca 《Plant Cell, Tissue and Organ Culture》1996,46(3):179-186
In vitro culture of barley calluses has been used to produce plants with increased glyphosate tolerance. Calluses from immature embryos of barleyHordeum vulgare L. (Jeff) were cultured on Murashige and Skoog medium with 10-6, 10-5, 10-4, 5×10-4, 10-3, or 10-2M glyphosate for one, four or thirty months. Plants were regenerated from calluses maintained in glyphosate medium at 10-6, 10-5 or 10-4M for four months, at 10-5 or 5×10-4M for one month and at 10-5M for thirty months. The progeny of each regenerated plant was analyzed for response to glyphosate. Some progenies showed increased tolerance to glyphosate. 相似文献
4.
Barragán-Ocaña Alejandro Reyes-Ruiz Gerardo Olmos-Peña Samuel Gómez-Viquez Hortensia 《Transgenic research》2019,28(3-4):391-399
Transgenic Research - Transgenic crops have been the recipient of strong support as well as vigorously opposed opinions since their appearance. In any case, their growth throughout the world has... 相似文献
5.
The RhoA effector mDia is induced during T cell activation and regulates actin polymerization and cell migration in T lymphocytes 总被引:4,自引:0,他引:4
Vicente-Manzanares M Rey M Pérez-Martínez M Yáñez-Mó M Sancho D Cabrero JR Barreiro O de la Fuente H Itoh K Sánchez-Madrid F 《Journal of immunology (Baltimore, Md. : 1950)》2003,171(2):1023-1034
Regulation of actin polymerization is critical for many different functions of T lymphocytes, including cell migration. Here we show that the RhoA effector mDia is induced in vitro in activated PBL and is highly expressed in vivo in diseased tissue-infiltrating activated lymphocytes. mDia localizes at the leading edge of polarized T lymphoblasts in an area immediately posterior to the leading lamella, in which its effector protein profilin is also concentrated. Overexpression of an activated mutant of mDia results in an inhibition of both spontaneous and chemokine-directed T cell motility. mDia does not regulate the shape of the cell, which involves another RhoA effector, p160 Rho-coiled coil kinase, and is not involved in integrin-mediated cell adhesion. However, mDia activation blocked CD3- and PMA-mediated cell spreading. mDia activation increased polymerized actin levels, which resulted in the blockade of chemokine-induced actin polymerization by depletion of monomeric actin. Moreover, mDia was shown to regulate the function of the small GTPase Rac1 through the control of actin availability. Together, our data demonstrate that RhoA is involved in the control of the filamentous actin/monomeric actin balance through mDia, and that this balance is critical for T cell responses. 相似文献
6.
Muelas S Suárez M Pérez R Rodríguez H Ochoa C Escario JA Gómez-Barrio A 《Memórias do Instituto Oswaldo Cruz》2002,97(2):269-272
Cytotoxicity assays of 24 new 3,5-disubstituted-tetrahydro-2H-1,3,5-thiadiazin-2-thione derivatives were performed. The 17 compounds with higher anti-epimastigote activity and lower cytotoxicity were, thereafter, screened against amastigote of Trypanosoma cruzi. Out of these 17 derivatives S-2d was selected to be assayed in vivo, because of its remarkable trypanocidal properties. To determine toxicity against J774 macrophages, a method based on quantification of cell damage, after 24 h, was used. Cell respiration, an indicator of cell viability, was assessed by the reduction of MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] to formazan. Anti-amastigote activity was estimated after 48 h by microscopic counts of May Grünwald-Giemsa-stained monolayers. Nifurtimox and benznidazole were used as reference drugs. For the in vivo experiences, mice were infected with 10(4) blood trypomastigotes and then treated during 15 days with S-2d or nifurtimox by oral route. All of the compounds were highly toxic at 100 micro g/ml for macrophages and a few of them maintained this cytotoxicity even at 10 microg/ml. Of the derivatives assayed against amastigotes 3k and S-2d showed an interesting activity, that was held even at 1microg/ml. It is demonstrated that the high anti-epimastigote activity previously reported is mainly due to the non-specific toxicity of these compounds. In vivo assays assessed a reduction of parasitemia after administration of S-2d to infected mice. 相似文献
7.
Recent developments in the characterization and biotechnological production of sweet-tasting proteins 总被引:12,自引:1,他引:11
Faus I 《Applied microbiology and biotechnology》2000,53(2):145-151
The state of the art regarding the six known sweet-tasting proteins (thaumatin, monellin, mabinlin, pentadin, brazzein and
curculin) and the taste-modifying protein miraculin is reviewed. Their biochemical properties, molecular genetics and biotechnological
production are assessed. All of these proteins have been isolated from plants that grow in tropical rainforests. They share
no sequence homology or structural similarities. Nonetheless, one of them, thaumatin, shares extensive homology with certain
non-sweet proteins found in other plants. The potential industrial applications of the sweet-tasting proteins are also discussed,
placing special emphasis on the barriers that a recombinant product of these characteristics will have to overcome before
it reaches the market.
Received: 14 June 1999 / Received revision: 30 August 1999 / Accepted: 3 September 1999 相似文献
8.
Previous studies have shown the presence of neuronal perikarya in the primate ovary, but not in the ovary from Sprague-Dawley rats. We report here that while such intrinsic neurons are indeed absent in this strain of rats, they can be visualized in the ovary from Wistar rats. The neurons, identified by their morphology and by the expression of NeuN (a neuron-specific nuclear protein), were detected at all postnatal intervals examined, from 14 h after birth to 50 days of age. While they were present in the ovarian hilum and medulla at all ages studied, neurons first appeared in the ovarian cortex during the juvenile period (postnatal days 10-20). In all cases, the size of the neuronal soma increased significantly during prepubertal development, reaching maximal values before puberty. Some neurons were catecholaminergic, as indicated by their content of immunoreactive tyrosine hydroxylase (TH), the rate-limiting enzyme of catecholamine biosynthesis. Some showed neuropeptide Y (NPY) immunoreactivity. TH-positive neurons were seen either in isolation or clustered in ganglion-like structures in both the ovarian cortex and medulla. These results indicate that ovarian neurons are not present in all strains of rats, but when present, the chemical phenotype of some of them is of a sympathetic nature, similar to that described in primates. 相似文献
9.
Hortensia Rodríguez Margarita Suarez Fernando Albericio 《Journal of peptide science》2010,16(3):136-140
Structural modification of the peptide backbone via N‐methylation is a powerful tool to modulate the pharmacokinetic profile and biological activity of peptides. Here we describe a rapid and highly efficient microwave(MW)‐assisted Fmoc/tBu solid‐phase method to prepare short chain N‐methyl‐rich peptides, using Rink amide p‐methylbenzhydrylamine (MBHA) resin as solid‐phase support. This method produces peptides in high yield and purity, and reduces the time required for Fmoc‐N‐methyl amino acid coupling. Copyright © 2010 European Peptide Society and John Wiley & Sons, Ltd. 相似文献
10.
Carmen Martínez José A Molina Hortensia Alonso-Navarro Félix J Jiménez-Jiménez José AG Agúndez Elena García-Martín 《BMC neurology》2010,10(1):71