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ElisabethSalier HeikeLaue Hans R. SchläfliOppenberg Alasdair M.Cook 《FEMS microbiology letters》1995,130(1):97-102
Abstract Inducible (1 R ,2 S )-1,2-dihydroxy-3,5-cyclohexadiene-l,4-dicarboxylate (diene-diol) dehydrogenase was found in extracts of Comamonas testosteroni T-2 grown in p -toluate-or terephthalate-salts medium and it was purified using anion exchange, hydrophobic interaction and gel filtration chromatography. The enzyme is a homodimer with subunit M r 39000. It had a specific activity of 500 mkat/kg of protein and was activated by the addition of Fe2+ . The dehydrogenase converted 1 mol diene-diol and 1 mol NAD+ to 1 mol protocatechuic acid, 1 mol NADH and 1 mol CO2 . Apparent K m -values of 43 μM (NAD+ ) and about 90 μM (diene-diol) were determined. The hydride ion was transferred to the si face of NAD+ . 相似文献
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