Cultivation of Mycobacterium tuberculosis in cell culture

The method of the cultivation of M .tuberculosis in cell subcultures was tested. Five consecutive passages of 2 M. tuberculosis strains were carried out with similar inoculation and cultivation conditions. Mycobacterial cells preserved their morphological characteristics in the course of all passages. The method of M. tuberculosis cultivation in subcultured cells made it possible to study of the physiology of M. tuberculosis under conditions, most approximated to the natural tuberculosis infection in humans.     

Adaptation of Bio-Layer Interferometry for Quantitative Assessment of the Vascular Endothelial Growth Factor Content in Cell-Conditioned Culture Medium

Biophysics - Quantitative determination of cytokines, chemokines, growth factors, and other soluble protein molecules in various biological fluids is a routine task of modern diagnostics and...     

Isolation and molecular characterization of the influenza virus A/H5N1 strains isolated during outbreak of avian influenza among birds in the European part of Russia in 2005: strain with ozeltamivir-resistance mutation was found

Isolation and characterization of the influenza virus A/H5N1 strains, isolated from chicken in the Yandovka village (Tula Region) and from wild swan near the orifice of the Volga River that died during an outbreak of avian flu in autumn 2005, were carried out. Genetic and phylogenetic analyses were performed. The goals of the analysis were to determine possible geographical origin of the strain, genetic similarity of isolated strains to earlier sequenced isolates, epidemic potential, existence of pathogenicity markers, and resistance to antiviral drugs. It was shown that the isolated influenza virus belonged to highly pathogenic variants of China origin by a reassortment of viruses genotypes Z and V circulated in poultry and wild birds. A number of molecular markers of pathogenicity to gallinaceous birds and mammals were found out. Mutations in the hemagglutinin gene promoting potentially high rate of replication in humans as well as mutations causing the resistance to amantadine/rimantadine were not found. The strain isolated from wild swan had the mutation causing resistance to tamiflu/ozeltamivir.     

Circulation of type A influenza viruses in poultry during the pre-epizootic period of 2005 in Novosibirsk region

Work is devoted to studying of circulation of avian flu influenza A (H5N1) virus at poultry in territory of Novosibirsk region in preepizootic period. 1901 sample from clinically healthy poultry is collected and investigated. On chicken embryos it has been isolated 28 virions, including from poultry of the industrial maintenance--16, from the poultry contained in private farmsteads--12. At the analysis of isolates it has been shown, that among poultry in preepizootic period circulated various serovars of influenza viruses namely, HI, H4 and H3 serogroups. The percent of isolates from geese of the industrial maintenance increased to 0.6% depending on area. The percent of isolates from a bird contained in private farmsteads, increased to 7.5%. The greatest percent of isolates was observed from the samples taken from hens--77. Thus, it is shown, that among poultry circulation of not pathogenic influenza viruses of type A is the usual phenomenon, and asymptomatic carriers 7.5% of a livestock. Also it has been established, that in preepizootic period 2005 (January-June) at poultry it is not revealed influenza viruses of H5 serotype, caused epizootia at Summer of 2005 in territory of Western Siberia.     

Genetical features of influenza virus A (H1N1) strain that caused the 2009 pandemic

Genetical features of the A(H1N1) influenza virus strain that caused the 2009 pandemic are analyzed in the review. Mutations typical for this strain, unique and similar to influenza viruses of swine, avian and seasonal types, and phenotypic (pathologic) features associated with them, that are experimentally confirmed, are described. A possibility of reassortation of avian and swine influenza viruses and possible epidemiologic consequences are discussed.     

Study of highly pathogenic H5N1 influenza virus isolated from sick and dead birds in Western Siberia

The mass destruction of domestic birds has been registered in July, 2005 in Novosibirsk region. Influenza virus H5N1 have been isolated from bodies of the lost birds on developing chicken embryos and identified by serological and molecular biological methods. M-gene and genes coding hemagglutinin and neurominidase were in part sequening. The phylogenetic analysis of hemagglutinin gene has shown, that the isolated viruses are forming a claster with strains, isolated from birds during outbreak of the bird's flu on lake Tsinghai (Qinghai) in China in 2005, in Japan in 2004, and also with H5N1 strains, isolated from the person and birds in the countries of Southeast Asia during ipizootia in 2003-2004. The site of the restriction which associated with pathogenicity of isolated avian influenza viruses H5 serogroup, corresponds to sequence of high pathogenic strains, circulating in the countries of Southeast Asia. The test for pathogenicity with use of chickens has confirmed, that researched strains were high pathogenic for birds.