排序方式: 共有7条查询结果,搜索用时 15 毫秒
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Darawan Rinchai Donporn Riyapa Surachat Buddhisa Kusumawadee Utispan Richard W Titball Mark P Stevens Joanne M Stevens Michinaga Ogawa Isei Tanida Masato Koike Yasuo Uchiyama Manabu Ato Ganjana Lertmemongkolchai 《Autophagy》2015,11(5):748-755
Neutrophils play a key role in the control of Burkholderia pseudomallei, the pathogen that causes melioidosis. Here, we show that survival of intracellular B. pseudomallei was significantly increased in the presence of 3-methyladenine or lysosomal cathepsin inhibitors. The LC3-flux was increased in B. pseudomallei-infected neutrophils. Concordant with this result, confocal microscopy analyses using anti-LC3 antibodies revealed that B. pseudomallei-containing phagosomes partially overlapped with LC3-positive signal at 3 and 6 h postinfection. Electron microscopic analyses of B. pseudomallei-infected neutrophils at 3 h revealed B. pseudomallei-containing phagosomes that occasionally fused with phagophores or autophagosomes. Following infection with a B. pseudomallei mutant lacking the Burkholderia secretion apparatus Bsa Type III secretion system, neither this characteristic structure nor bacterial escape into the cytosol were observed. These findings indicate that human neutrophils are able to recruit autophagic machinery adjacent to B. pseudomallei-containing phagosomes in a Type III secretion system-dependent manner. 相似文献
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Maskiet Boonyareth Jureepan Saranak Darawan Pinthong Yupin Sanvarinda Kenneth W. Foster 《Biologia》2009,64(6):1058-1065
Chlamydomonas reinhardtii swims toward or away from light (phototaxis) in a graded way depending on various conditions. Activation of rhodopsin provides
signals to control the steering of this unicellular organism relative to a light source and to up-regulate rhodopsin biosynthesis.
Intracellular cAMP and cGMP concentrations were measured in positive (1117, swims toward light) and negative (806, swims away
from light) phototactic strains with and without light stimulation or 3-isobutyl-1-methylxanthine (IBMX). In the dark, the
levels of cAMP and cGMP were significantly higher in the strain with positive phototaxis than in the strain with negative
phototaxis. To test whether either cyclic nucleotide influenced the direction, their pre-stimulus levels were pharmacologically
manipulated. Higher pre-stimulus levels of cAMP biased the cells to swim toward green light and lower levels biased the cells
to swim away. In addition, green-light activation of rhodopsin or addition of IBMX causes a sustained increase in cAMP in
both strains. As a consequence of this increase in cAMP, carotenogenesis is induced, as shown by recovery of phototaxis in
a carotenoid mutant. Thus, two functions for cAMP were identified: high pre-stimulus level biases swimming toward a light
source and sustained elevation following rhodopsin activation increases rhodopsin biosynthesis. 相似文献
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Suputtamongkol Y Premasathian N Bhumimuang K Waywa D Nilganuwong S Karuphong E Anekthananon T Wanachiwanawin D Silpasakorn S 《PLoS neglected tropical diseases》2011,5(5):e1044
Background
Strongyloidiasis, caused by an intestinal helminth Strongyloides stercoralis, is common throughout the tropics. It remains an important health problem due to autoinfection, which may result in hyperinfection and disseminated infection in immunosuppressed patients, especially patients receiving chemotherapy or corticosteroid treatment. Ivermectin and albendazole are effective against strongyloidiasis. However, the efficacy and the most effective dosing regimen are to be determined.Methods
A prospective, randomized, open study was conducted in which a 7-day course of oral albendazole 800 mg daily was compared with a single dose (200 microgram/kilogram body weight), or double doses, given 2 weeks apart, of ivermectin in Thai patients with chronic strongyloidiasis. Patients were followed-up with 2 weeks after initiation of treatment, then 1 month, 3 months, 6 months, 9 months, and 1 year after treatment. Combination of direct microscopic examination of fecal smear, formol-ether concentration method, and modified Koga agar plate culture were used to detect strongyloides larvae in two consecutive fecal samples in each follow-up visit. The primary endpoint was clearance of strongyloides larvae from feces after treatment and at one year follow-up.Results
Ninety patients were included in the analysis (30, 31 and 29 patients in albendazole, single dose, and double doses ivermectin group, respectively). All except one patient in this study had at least one concomitant disease. Diabetes mellitus, systemic lupus erythrematosus, nephrotic syndrome, hematologic malignancy, solid tumor and human immunodeficiency virus infection were common concomitant diseases in these patients. The median (range) duration of follow-up were 19 (2–76) weeks in albendazole group, 39 (2–74) weeks in single dose ivermectin group, and 26 (2–74) weeks in double doses ivermectin group. Parasitological cure rate were 63.3%, 96.8% and 93.1% in albendazole, single dose oral ivermectin, and double doses of oral ivermectin respectively (P = 0.006) in modified intention to treat analysis. No serious adverse event associated with treatment was found in any of the groups.Conclusion/Significance
This study confirms that both a single, and a double dose of oral ivermectin taken two weeks apart, is more effective than a 7-day course of high dose albendazole for patients with chronic infection due to S. stercoralis. Double dose of ivermectin, taken two weeks apart, might be more effective than a single dose in patients with concomitant illness.Trial Registration
ClinicalTrials.gov NCT00765024相似文献5.
Arnone Nithichanon Darawan Rinchai Alessandro Gori Patricia Lassaux Claudio Peri Oscar Conchillio-Solé Mario Ferrer-Navarro Louise J. Gourlay Marco Nardini Jordi Vila Xavier Daura Giorgio Colombo Martino Bolognesi Ganjana Lertmemonkolchai 《PLoS neglected tropical diseases》2015,9(7)
Burkholderia pseudomallei is a Gram-negative bacterium responsible for melioidosis, a serious and often fatal infectious disease that is poorly controlled by existing treatments. Due to its inherent resistance to the major antibiotic classes and its facultative intracellular pathogenicity, an effective vaccine would be extremely desirable, along with appropriate prevention and therapeutic management. One of the main subunit vaccine candidates is flagellin of Burkholderia pseudomallei (FliCBp). Here, we present the high resolution crystal structure of FliCBp and report the synthesis and characterization of three peptides predicted to be both B and T cell FliCBp epitopes, by both structure-based in silico methods, and sequence-based epitope prediction tools. All three epitopes were shown to be immunoreactive against human IgG antibodies and to elicit cytokine production from human peripheral blood mononuclear cells. Furthermore, two of the peptides (F51-69 and F270-288) were found to be dominant immunoreactive epitopes, and their antibodies enhanced the bactericidal activities of purified human neutrophils. The epitopes derived from this study may represent potential melioidosis vaccine components. 相似文献
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Praphathip Eamsobhana Darawan Wanachiwanawin Naowarat Dechkum Anchana Parsartvit Hoi Sen Yong 《Memórias do Instituto Oswaldo Cruz》2013,108(1):116-118
Cerebrospinal fluid (CSF) samples from clinically diagnosed patients with detectable Angiostrongylus canto-nensis-specific antibodies (n = 10), patients with clinically suspected cases that tested negative for A. cantonensis-an-tibodies (n = 5) and patients with cerebral gnathostomiasis (n = 2) and neurocysticercosis (n = 2) were examined by a single-step polymerase chain reaction (PCR) method using the AC primers for the 66-kDa native protein gene. The PCR method detected A. cantonensis DNA in CSF samples from four of 10 serologically confirmed angiostrongyliasis cases. The PCR results were negative for the remaining CSF samples. The nucleotide sequences of three positive CSF-PCR samples shared 98.8-99.2% similarity with the reference sequence of A. cantonensis. These results indicate the potential application of this PCR assay with clinical CSF samples for additional support in the confirmation of eosinophilic meningitis due to A. cantonensis. 相似文献
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