排序方式: 共有17条查询结果,搜索用时 343 毫秒
1.
Vazquez-Marquez Alicia Monserrat Zepeda-Gómez Carmen Burrola-Aguilar Cristina Bernabé-Antonio Antonio Nieto-Trujillo Aurelio Cruz-Sosa Francisco Rodríguez-Monroy Mario Estrada-Zúñiga María Elena 《Plant Cell, Tissue and Organ Culture》2019,139(1):155-166
Plant Cell, Tissue and Organ Culture (PCTOC) - Shake-flask in vitro culture of Buddleja cordata cells produces large amounts of biomass and synthetizes verbascoside (VB), linarin and... 相似文献
2.
J. L. Trejo-Espino M. Rodríguez-Monroy E. J. Vernon-Carter F. Cruz-Sosa 《Acta Physiologiae Plantarum》2011,33(5):1687-1695
This study presents a protocol for the establishment of Prosopis laevigata cell suspension culture as a strategy to obtain an in vitro mesquite gum productive cell line. The callus used for this purpose
was obtained with hypocotyls from 15-day-old plantlets, placed on Murashige–Skoog medium with two different plant growth regulators
(PGRs), 2,4,5-trichlorophenoxy acetic acid (2,4,5-T; 5.0 μM) and kinetin (KIN; 5.0 μM). With this PGRs treatment, after four
subcultures (30 days each) an exuded gum-like substance was observed on the callus surface. The growth kinetics of the cell
suspension culture showed a specific cell growth rate (μ) of 0.14 d−1 and doubling time (t
d) of 6.6 days, respectively. The gum-like substance from callus culture and the broth from cell suspension culture were subjected
to chemical analysis and compared with the mesquite gum exuded from wild trees. Both, gum-like substance from callus culture
and the broth from cell suspension culture showed the presence of Arabinogalactan-proteins, and their polysaccharide fraction
presented the same monosaccharides as those isolated from mesquite gum. In addition, the emulsifying properties of gum-like
substance from callus culture and the broth from cell suspension culture were compared to those of mesquite gum and all three
samples exhibited similar emulsifying capacity and emulsification stability. 相似文献
3.
G. Salcedo-Morales A. R. Jiménez-Aparicio F. Cruz-Sosa G. Trejo-Tapia 《Biologia Plantarum》2014,58(1):164-168
In vitro induction of haustoria from Castilleja tenuiflora Benth. was achieved by applying 25 μM catechin, 25 μM vanillin, or 25 μM H2O2. Of the treatments tested, 25 μM vanillin was the strongest inducer of haustoria in C. tenuiflora roots in vitro (up to 3 haustoria per root). Haustorium development occurred laterally and was observable 14 d after inducer application. It was characterized by elongation of the epidermal cells and division of the inner cortical cells which also possessed abundant nuclei. Histochemical analysis using 3,3-diaminobenzidine (DAB) and diphenylboric acid 2-aminoethyl ester (DBPA) indicated that the formation of haustoria was associated with the accumulation of H2O2 and flavonoids. 相似文献
4.
Juan Orozco-Villafuerte Leticia Buendía-González Francisco Cruz-Sosa Eduardo J Vernon-Carter 《Plant Physiology and Biochemistry》2005,43(8):802-807
Prosopis laevigata nodal explants cultures were established in Murashige and Skoog medium. Simultaneously these cultures were subjected to stress with biotic elicitors and an environmental factor (temperature increase to promote heat stress) in order to promote and increase exuded mesquite gum production. The biotic elicitors were: Aspergillus nidulans and Pseudomonas pseudoalcaligenes both used in concentrations of 10, 20 and 30 mg, whereas the environmental condition was different incubation temperatures (25, 35 and 40 degrees C). The greatest gum production (approximately 13 mg of pooled gum from 100 explants after 14 days incubation) took place when the culture medium was added 10, 20 and 30 mg of autoclaved fungal mycelium of A. nidulans or 30 mg of autoclaved bacterial biomass of P. pseudoalcaligenes in combination with an incubation temperature of 35 degrees C. These treatments were non-significantly different among themselves (P < 0.05), but were significantly different to the rest of the treatments (P > 0.05). 相似文献
5.
María del Pilar Nicasio-Torres Mariana Meckes-Fischer Lucía Aguilar-Santamaría María Luisa Garduño-Ramírez Víctor Manuel Chávez-Ávila Francisco Cruz-Sosa 《Acta Physiologiae Plantarum》2012,34(1):307-316
The antidiabetic properties of Cecropia obtusifolia are attributed to chlorogenic acid (CGA) and isoorientin (ISO) phenolic compounds; both compounds possess hypoglycemic, hypolipidemic,
and antioxidant properties. As a potential strategy for an adequate supply of authentic plant raw material, the aim of this
study was to establish in vitro conditions for the development of cell suspension cultures that produce these bioactive compounds.
Callus cultures of leaf explants from acclimatized tree and in vitro plantlets were set up using different auxin levels; treatments
with 2,4-dichlorophenoxyacetic acid (2,4-D) and α-naphthalene acetic acid (NAA) to 8.92 μM with 6-benzylaminopurine (BAP)
at 2.22 μM stimulate highest callus production. Seedling cotyledon, hypocotyl, leaf, and stem explants developed calli bearing
roots with 2,4-D. With NAA, hypocotyl, cotyledon, and leaf explants developed morphogenic calli; 75% of stem explants formed
calli, and the remaining calli developed shoots. Determined CGA concentrations in calli were similar to those detected in
the leaves of wild trees, and ISO was not produced. Cell suspension cultures were established from leaf explants friable calli
with 8.92 μM 2,4-D in combination with 2.22 μM BAP, employing 4 and 5% inocula in fresh weight; CGA levels were maintained
and ISO was produced only at the end of logarithmic growth. On diminishing nitrate content in Murashige and Skoog (MS) medium
to 8.0 mM, maximum cell biomasses diminished, CGA production is increased and twice with 16.0 and, instead of CGA production
is tripled and quadrupled with 16.0 and 8.0 mM nitrates, respectively, and ISO synthesis was induced earlier and for a longer
time period, increasing its levels at the end of culture. Two compounds with ultraviolet spectra similar to those of caffeic
and ferulic acids were formed. Our results offer a protocol of cell suspension cultures for C. obtusifolia bioactive production and hypoglycemic property conservation. 相似文献
6.
Nadia Tapia Alejandro Zamilpa Mercedes Bonfill Elsa Ventura Delia Cruz-Vega Alma Del Villar Francisco Cruz-Sosa Lidia Osuna 《Acta Physiologiae Plantarum》2013,35(12):3447-3455
Taxus globosa is the only species of the Taxus genus that grows in Mexico. In this study, callus cultures from leaves and young shoots of T. globosa were established in Gamborg’s B5 medium supplemented with 2,4-dichlorophenoxiacetic acid (2 mg/L), kinetin (0.5 mg/L) and gibberellic acid (0.25 mg/L). Callus growth and taxane production were evaluated using two culture media: Woody Plant Medium and Gamborg’s B5 supplemented with picloram (2 mg/L), kinetin (0.1 mg/L) and gibberellic acid (0.5 mg/L). The effect of the inoculum size (50, 100 and 150 g FW/L) and culture media (Woody Plant Medium and Gamborg’s B5) with and without the presence of methyl jasmonate (100 μM) on T. globosa cell suspensions was assessed. Taxane analysis revealed that the calli in Gamborg’s B5 produced taxol (50 μg/g DW), baccatin III, 10-deacetyl baccatin III and 10-deacetyl taxol. Woody Plant Medium also induced the production of taxol, although to a lesser extent. The optimum inoculum size was 50 g FW/L. In cell suspension cultures, both media had a significant effect on taxane production when supplemented with methyl jasmonate. In Woody Plant Medium, at day 14, a total concentration of 197.999 μg/L of taxol, 160.622 μg/L of baccatin III, 633.724 μg/L of 10-deacetyl baccatin III and 229.611 μg/L 10-deacetyl taxol were obtained, with total excretion of baccatin III and 10-deacetyl taxol to the culture medium. In Gamborg’s B5, cephalomanine was obtained at a concentration of 91.428 μg/L without elicitation, and all taxanes were excreted to the medium to a variable extent. 相似文献
7.
M. E. Estrada-Zúñiga F. Cruz-Sosa M. Rodríguez-Monroy J. R. Verde-Calvo E. J. Vernon-Carter 《Plant Cell, Tissue and Organ Culture》2009,97(1):39-47
Plant tissue cultures represent a potential source for producing secondary metabolites. In this work, Buddleja cordata tissue cultures were established in order to produce phenylpropanoids (verbascoside, linarin and hydroxycinnamic acids),
as these metabolites are credited with therapeutic properties. Highest callus induction (76.4–84.3%) was obtained in five
treatments containing 2,4-Dichlorophenoxyacetic acid (2,4-d: 0.45–9.05 μM) with Kinetin (KIN: 2.32, 4.65 μM), whereas highest root induction (79.6%) corresponded to the α-Naphthaleneacetic
acid (9.05 μM) with KIN (2.32 μM) treatment. Verbascoside was the major phenylpropanoid produced in in vitro cultures (root,
white and green callus) [66.24–86.26 mg g−1 dry weight (DW)], while linarin and hydroxycinnamic acid production was low (0.95–3.01 mg g−1 DW). Verbascoside and linarin production were improved in cell suspension culture (116 mg g−1 DW and 8.12 mg g−1 DW, respectively). 相似文献
8.
Reynoso-Cuevas L Gallegos-Martínez ME Cruz-Sosa F Gutiérrez-Rojas M 《Bioresource technology》2008,99(14):6379-6385
The effect of a hydrocarbon mixture (HCM) of three polycyclic aromatic hydrocarbons (PAH) and Maya crude oil on germination, growth and survival of four grasses (Bouteloua curtipendula, Cenchrus ciliaris, Echinochloa crusgalli and Rhynchelytrum repens) was studied and compared to a control (Festuca arundinacea) under in vitro conditions. The species were cultured on MS medium with different HCM initial concentrations. Germination was not affected for any assayed concentration; however, the length of the stems and roots decreased when HCM increased and the survival of the four species also diminished. Except for F. arundinacea, a direct link between hydrocarbon concentration and plant survival was observed. In vitro studies are clean and easy to handle techniques allowing isolation of the plant activity from that derived from associations with microorganisms in non-sterile cultures. To our knowledge, this is the first work towards phytoremediation assisted by in vitro plant cultivation. 相似文献
9.
María del Pilar Nicasio-Torres Juanita Pérez-Hernández Manasés González-Cortazar Mariana Meckes-Fischer Jaime Tortoriello Francisco Cruz-Sosa 《Acta Physiologiae Plantarum》2016,38(8):209
Sphaeralcea angustifolia is used in Mexican traditional medicine to treat inflammatory processes. SCopoletin (SC), TOmentin (TO), and sphaeralcic acid (SA) were reported as the main anti-inflammatory compounds in this species. The aim of this study was to establish in vitro conditions for the development of calli and cell suspension cultures that are the producers of these active compounds. Callus cultures of plant leaf explants were set up using different auxin levels of α-naphthalene acetic acid (NAA) in combination with a constant concentration (0.1 mg L?1) of Kinetin (Kn) in Murashige and Skoog (MS) medium. Optimal combinations for callus induction were 1.0 and 2.0 mg L?1 of NAA. SC, TO, and SA were not detected in callus tissues. Employing a 4 % inoculum in fresh biomass, cell suspension was established from friable callus with 1.0 mg L?1 of NAA in combination with 0.1 mg L?1 of Kn in MS liquid medium (27.4 mM nitrate). The cellular suspension synthesized SC and SA, SC was excreted into the culture medium, while SA was excreted into the culture medium and accumulated in biomass. To improve SC and SA production, total nitrate content was reduced in MS medium. On diminishing nitrate content to 2.74 mM, cellular suspension growth was not modified. SC concentration (0.04 %) was 60-fold higher than that detected in the wild plant (0.00067 %), TO was produced (0.096 %), and SA content (0.0036 %) was not improved. SA production in MS medium with 0.274 mM nitrate (0.004 %) was enriched 12-fold (0.0003 %) in relation to that of the wild plant. The anti-inflammatory effects at 5 h of intraperitoneal (i.p.) administration (100 mg per kg BW) of dichloromethane extracts from the medium (42 ± 3 %) and biomass (39 ± 9.3 %) of S. angustifolia cell suspensions cultivated in MS with 2.74 mM nitrate were similar. The effect of the biomass dichloromethane extract was dose dependent with a median Effective Dose (ED50) of 137.63 mg per kg BW. 相似文献
10.
Gum arabic-chitosan complex coacervation 总被引:1,自引:0,他引:1
Espinosa-Andrews H Báez-González JG Cruz-Sosa F Vernon-Carter EJ 《Biomacromolecules》2007,8(4):1313-1318
The formation of electrostatic complexes of gum Arabic (GA) with chitosan (Ch), two oppositely charged polysaccharides, as a function of the biopolymers ratio (RGA/Ch), total biopolymers concentration (TBconc), pH, and ionic strength, was investigated. The conditions under which inter-biopolymer complexes form were determined by using turbidimetric and electrophoretic mobility measurements in the equilibrium phase and by quantifying mass in the precipitated phase. Results indicated that optimum coacervate yield was achieved at RGA/Ch = 5, independently of TBconc at the resulting pH of solutions under mixing conditions. High coacervate yields occurred in a pH range from 3.5 to 5.0 for RGA/Ch = 5. Coacervate yield was drastically diminished at pH values below 3.5 due to a low degree of ionization of GA molecules, and at pH values above 5 due to a low solubility of chitosan. Increasing ionic strength decreased coacervate yield due to shielding of ionized groups. 相似文献