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1.
We compare the chromosomal 28S and 5S rDNA patterns of the spined loach C. taenia (2n = 48) from an exclusively diploid population and from a diploid–polyploid population using 28S and 5S rDNA probe preparation
and labelling, and fluorescence in situ hybridization (FISH). The 5S rDNA was located in two to three chromosome pairs, and separated from the 28S loci for the males
and one female (F1) from the diploid population. Loaches from a diploid–polyploid population, and one female (F2) from the
diploid population were characterized by at least one chromosome pair with 5S and 28S overlapping signals. The fishes differed
mainly in their number of 28S rDNA loci, located on 3–6 chromosomes. All individuals from both populations were characterized
by one acrocentric chromosome bearing a 28S rDNA signal on the telomeres of its long arm. The number of major ribosomal DNA
in the karyotype of C. taenia by FISH was always higher than the number of Ag-NORs. Our data confirm the extensive polymorphism of NORs in both populations,
as already has been observed in closely related Cobitis species, and less polymorphic 5S rDNA pattern. However, this preliminary result highlights the need for a wider scale study. 相似文献
2.
Hong H Coutanceau E Leclerc M Caleechurn L Leadlay PF Demangel C 《PLoS neglected tropical diseases》2008,2(10):e325
Background
Buruli ulcer (BU) is a progressive disease of subcutaneous tissues caused by Mycobacterium ulcerans. The pathology of BU lesions is associated with the local production of a diffusible substance, mycolactone, with cytocidal and immunosuppressive properties. The defective inflammatory responses in BU lesions reflect these biological properties of the toxin. However, whether mycolactone diffuses from infected tissues and suppresses IFN-γ responses in BU patients remains unclear.Methodology/Principal Findings
Here we have investigated the pharmacodistribution of mycolactone following injection in animal models by tracing a radiolabeled form of the toxin, and by directly quantifying mycolactone in lipid extracts from internal organs and cell subpopulations. We show that subcutaneously delivered mycolactone diffused into mouse peripheral blood and accumulated in internal organs with a particular tropism for the spleen. When mice were infected subcutaneously with M. ulcerans, this led to a comparable pattern of distribution of mycolactone. No evidence that mycolactone circulated in blood serum during infection could be demonstrated. However, structurally intact toxin was identified in the mononuclear cells of blood, lymph nodes and spleen several weeks before ulcerative lesions appear. Importantly, diffusion of mycolactone into the blood of M. ulcerans–infected mice coincided with alterations in the functions of circulating lymphocytes.Conclusion
In addition to providing the first evidence that mycolactone diffuses beyond the site of M. ulcerans infection, our results support the hypothesis that the toxin exerts immunosuppressive effects at the systemic level. Furthermore, they suggest that assays based on mycolactone detection in circulating blood cells may be considered for diagnostic tests of early disease. 相似文献3.
Marsollier L Aubry J Coutanceau E André JP Small PL Milon G Legras P Guadagnini S Carbonnelle B Cole ST 《Cellular microbiology》2005,7(7):935-943
Mycobacterium ulcerans was first identified as the causative agent of Buruli ulcer; this cutaneous tissue-destructive process represents the third most important mycobacterial disease in humans after tuberculosis and leprosy. More recently other life traits were documented. M. ulcerans is mainly detected in humid tropical zones as part of a complex ecosystem comprising algae, aquatic insect predators of the genus Naucoris, and very likely their vegetarian preys. Coelomic plasmatocytes could be the first cells of Naucoris cimicoides to be involved in the infection process, acting as shuttle cells that deliver M. ulcerans to the salivary glands as suggested by both in vitro and in vivo approaches. Furthermore, a key element for the early and long-term establishment of M. ulcerans in Naucoridae is demonstrated by the fact that only mycolactone toxin-producing M. ulcerans isolates are able to invade the salivary glands, a site where they proliferate. Later, the raptorial legs of Naucoris are covered by M. ulcerans-containing material that displays features of biofilms. 相似文献
4.
Federico Mazzei Laura Ghigliotti Guillaume Lecointre Catherine Ozouf-Costaz Jean-Pierre Coutanceau William Detrich III Eva Pisano 《Polar Biology》2006,29(12):1071-1076
Using comparative cytogenetic techniques, we characterized the chromosomes of fishes from the family Bovichtidae, the basal lineage of the largely Antarctic suborder Notothenioidei. We focused on three Sub-Antarctic species of the genus Bovichtus that differ greatly in their circumpolar distributions: B. diacanthus (Tristan da Cunha Island Group), B. variegatus (New Zealand) and B. angustifrons (Tasmania). Chromosomes were analyzed both by conventional karyotyping and by cytogenetic mapping of ribosomal genes using fluorescence in situ hybridization. The three species displayed a strongly conserved karyotype consisting entirely of telocentric chromosomes (diploid number = 48; Fundamental Number = 48), in agreement with our previously published hypothesis that the bovichtid karyotype is the basal state for notothenioid fishes. The chromosomal distribution of ribosomal genes differed from those of most notothenioid species studied to date, with the 45S and 5S genes separated on two different chromosome pairs. Separation of two classes of ribosomal genes is the most widespread condition in teleosts, including the bovichtids. Most notothenioid lineages on the other hand exhibit a derived consolidation of these genes. 相似文献
5.
Coutanceau E Legras P Marsollier L Reysset G Cole ST Demangel C 《Microbes and infection / Institut Pasteur》2006,8(8):2075-2081
Buruli ulcer, a disease caused by Mycobacterium ulcerans, is emerging as an increasingly important cause of morbidity throughout the world, for which surgery is the only efficient treatment to date. The aim of this work was to identify potential vaccine candidates in an experimental model of mouse infection. In BALB/c mice infected with M. ulcerans subcutaneously, Hsp65 appeared to be an immunodominant antigen eliciting both humoral and cellular responses. However, vaccination of mice with a DNA vector encoding Mycobacterium leprae Hsp65 only poorly limited the progression of M. ulcerans infection. In contrast, a substantial degree of protection was conferred by subcutaneous vaccination with BCG, suggesting that BCG antigens that are conserved in M. ulcerans, such as TB10.4, the 19 kDa antigen, PstS3 and Hsp70, may be interesting to consider as subunit vaccines in future prospects. 相似文献
6.
Pisano E Coscia MR Mazzei F Ghigliotti L Coutanceau JP Ozouf-Costaz C Oreste U 《Genetica》2007,130(1):9-17
The chromosomal location of the IgH locus has been analyzed in several bony fish of the Antarctic perciform group Notothenioidei.
Two IgH probes were prepared from the species Trematomus bernacchii (family Nototheniidae, tribe Trematominae) and mapped onto the chromosomes of ten species belonging to the same genus (Trematomus) and in two outgroups, through one-color and two-color FISH. A single location of the IgH locus was found in the majority
of the species examined, including the outgroups, whereas in four of them the IgH genes splited to two chromosomal loci. RT-PCR
experiments revealed the presence of three allelic sequences in T. newnesi, a species in which the IgH genes were organized in two chromosomal loci. Possible pathways leading to IgH genes duplication
during the diversification of trematomine fishes were inferred from the analysis of the FISH patterns in a phylogenetic context.
The present work provides the first comprehensive picture of IgH genes organization at chromosomal level in a bony fish group. 相似文献
7.
Lee BY Coutanceau JP Ozouf-Costaz C D'Cotta H Baroiller JF Kocher TD 《Marine biotechnology (New York, N.Y.)》2011,13(3):557-562
Identification of the sex-determining genes of the Nile tilapia (Oreochromis niloticus) has important implications for commercial aquaculture. We previously identified an XX/XY sex-determining locus in this species
within a 10-cM interval between markers GM201 and UNH995 on linkage group one (LG1). In order to refine this region, we developed
new AFLP markers using bulked segregant analysis of the mapping families. We identified three AFLP markers that showed a sex-specific
pattern of segregation. All three mapped near, but just outside, the previously identified sex-determining region on LG1.
Hybridization of BAC clones containing these markers to chromosome spreads confirmed that the XX/XY sex-determining locus
is on one of the small chromosomes in O. niloticus. 相似文献
8.
Géant E Mouchel-Vielh E Coutanceau JP Ozouf-Costaz C Deutsch JS 《Development genes and evolution》2006,216(7-8):443-449
The “hopeful monster” has haunted evolutionary thinking since Richard Goldschmidt coined the phrase in 1933. The phrase is directly related to genetic mechanisms in development and evolution. Cirripedes are peculiar crustaceans in that they all lack abdomens as adults. In a previous study aimed at describing the repertoire of Hox genes of the Cirripedia, we failed to isolate the abdominal-A gene in three species representative of all three cirripede orders. To address the question of whether the cirripede ancestor could have been a “hopeful monster” arising from a rearrangement of the Hox complex, we have performed a cytogenetic analysis of the Hox complex of the cirripede Sacculina carcini. We present here molecular and cytogenetic evidence for the grouping of the Hox genes on a single chromosome. This is the first direct evidence reported for the grouping of Hox genes on the same chromosome in a non-insect arthropod species. 相似文献
9.
Because of its unusual high degree of compaction and paucity of repetitive sequences, the genome of the smooth pufferfish Tetraodon nigroviridis is the subject of a well-advanced sequencing project. An astonishing diversity of transposable elements not found in the human and the mouse has been observed in the genome of T. nigroviridis. Due to the difficulty of assembling repeat-rich regions, the whole genome shotgun sequencing approach will probably fail to reveal the general organisation of this compact vertebrate genome. Therefore, in order to gain new insights into the global distribution pattern of repeated DNA in the genome of T. nigroviridis, we have reconstructed partial/complete repetitive sequences from data generated by the genome project and performed double-colour fluorescent in situ hybridization (FISH) analysis for representatives of three major categories of repeated sequences including two minisatellites (ms100 and ms104), two DNA transposons (Tol2 and Buffy1) and two non-long terminal repeat (LTR) retrotransposons (Rex3 and Babar). We show that DNA transposons and retroelements very frequently colocalize with minisatellites and mostly accumulate within heterochromatic regions. These results, which have not been reported so far for the fugu Takifugu rubripes, show that repeated elements are generally excluded from gene-rich regions in T. nigroviridis and underline the extreme degree of compartmentalization of this compact genome. The genome organization of the pufferfish is clearly different from that observed in humans, where repeated sequences make up an important fraction of euchromatic DNA, and is more similar to that observed in the fruit fly Drosophila melanogaster. 相似文献
10.