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Philippe-Alexandre Gilbert Lacrimioara Comanita John Barrett Andrew Peters Marta Szabat Grant McFadden Gregory A. Dekaban 《Cytotechnology》2005,48(1-3):79-88
In light of the recent detection of BSE in North America and its endemic nature in other regions of the world, there is a
real need to employ cell culture conditions that do not require any animal-derived material. Here we report the use of an
ultra-low protein serum-free medium (VP-SFM, Invitrogen) for the amplification of poxviruses in primary chicken embryo fibroblasts
(CEF). We compared the amplification of four different poxviruses (canarypox, modified Ankara Virus (MVA), vaccinia virus
strain Copenhagen and myxoma strain Lausanne) in three different media: DMEM 10%, DMEM 2% and serum-free medium VP-SFM. VP-SFM
is a serum-free, ultra-low protein medium containing no proteins or peptides of human or animal origin designed to support
the replication of viruses and the production of recombinant proteins and monoclonal antibodies. Our results show that high
titre poxvirus stocks can be prepared in VP-SFM equivalent to that prepared in serum containing medium. 相似文献
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CPG ODN allows lower dose of antigen against hepatitis B surface antigen in BALB/c mice 总被引:6,自引:0,他引:6
We have evaluated alum, immunostimulatory cytosine guanine dinucleotide-containing oligodeoxy-nucleotides (CPG ODN), and an alum/CPG ODN combination as adjuvants with hepatitis B surface antigen, to compare their potential to allow lower doses of antigen to be used for induction of humoral responses. BALB/c mice were immunized by intramuscular injection with 0.01, 0.1 or 1.0 micro g recombinant hepatitis B surface antigen without adjuvant or with alum and/or CPG ODN added. When given without adjuvant or with alum, each 10-fold decrease in amount of antigen resulted in a similarly reduced titre of antibody against hepatitis B surface antigen. In contrast, CPG ODN, on its own or combined with alum, allowed high anti-hepatitis B surface antigen titres even with a 1000-fold reduction in amount of antigen. These findings may have important immunological and economical consequences for vaccine development. 相似文献
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