On line continuous monitoring of blood lactate in men by a wearable device based upon an enzymatic amperometric lactate sensor

A wearable device for the continuous measurement of lactate in the blood was constructed by the combination of continuous blood sampling employing a double lumen catheter with an amperometric lactate sensor. In vitro, the lactate sensor turned out to have a linear concentration range between 0 and 15 mmol/l. The response time of the sensor itself amounted to 100 sec, whereas the lag time for blood sampling amounted to 2·2 min. In vivo, the lactate sensor was successfully used for the detection of changes of the blood lactate concentration following strenuous exercise in 7 healthy volunteers, in two cases up to 22 h. In conclusion, the technique of continuous blood sampling by the use of a double lumen catheter in combination with the amperometric lactate sensor is feasible and simplifies frequent blood lactate estimations.     

Lactobacillus lactis protoplasts at different stages of cell cultivation

Abstract Protein profiles of both protoplasts and cell homogenates of Lactobacillus lactis prepared in the course of cell multiplication were compared. The sodium dodecyl sulfate acrylamide gel electrophoretograms of whole cell homogenates and protoplasts show almost the same complexity. Protoplast protein profiles after 4 hours of growth exhibit new bands in both the low and high molecular mass region of the gel. The changes in protein composition during conversion of the cells into protoplasts are predominantly quantitative in nature.     

Sleep measurements in women with dysthymic disorder and insomnia

Sleep and Biological Rhythms - To determine the effects of trazodone, mirtazapine and hydroxyzine on sleep profiles of dysthymic disorder (DD) women with selective serotonin reuptake inhibitor...