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Cyanobacteria belonging mainly to the genera Anabaena and Oscillatoria were isolated from water-bloom spots of a sulfur spring in Staraya Matsesta. Their suspensions evolved O2 at a rate of 6--8 nM/min per 1 mg of dry cell weight at an intensity of solar radiation being 60--75 mV/cm2 per 1 sec. The cells were also capable of CO2 photoassimilation in the presence of solfide at a rate of 10(-4) mg C per mg per hour. DCMU at a concentration of 10(-5) M completely inhibited O2 evolution and inhibited CO2 fixation by 80%. Oxygen assimilation in dark by the suspensions did not depend on the addition of cyanide and was caused apparently by nonenzymatic reduction of O2 with sulfide dissolved in the spring water. Oxygen assimilation by the suspensions in light in the presence of DCMU was by 20--30% greater than in dark. Therefore, the cells of cyanobacteria are characterized by photorespiration at the level of photosystem I. Presumably, sulfide at a concentration of 9 mM cannot significantly inhibit the photosynthetic processes in cyanobacteria producing water-bloom spots in the sulfur spring. 相似文献
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Levashov PA Popov DV Popova VM Zhilenkov EL Morozova OA Belogurova NG Sedov SA Dyatlov IA Klyachko NL Levashov AV 《Biochemistry. Biokhimii?a》2010,75(9):1160-1164
Bacteriophage enzyme preparations exolysin and endolysin were studied. Exolysin (a phage-associated enzyme) was obtained from
tail fraction and endolysin from phage-free cytoplasmic fraction of disintegrated Salmonella enteritidis cells. A new method for purification of these enzymes was developed, and their molecular masses were determined. The main
catalytic properties of the studied enzymes (pH optimum and specificity to bacterial substrates) were found to be similar.
Both enzymes lyse Escherichia coli cells like chicken egg lysozyme, but more efficiently lyse S. enteritidis cells and cannot lyse Micrococcus luteus, a good substrate for chicken egg lysozyme. Similar properties of exolysin and endolysin suggest that these enzymes are structurally
similar or even identical. 相似文献
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S. A. Sedov N. G. Belogurova S. V. Shipovskov M. V. Semenova M. M. Gitinov A. V. Levashov P. A. Levashov 《Russian Journal of Bioorganic Chemistry》2012,38(3):274-281
Bacteriolytic factors from the blood plasma of healthy sheep have been studied. Three enzymes not described earlier in the literature have been identified and characterized. Two enzymes exhibit activity toward Escherichia coli and have molecular weights of 15 ± 2 kDa. The third enzyme that exhibits activity toward E. coli and Micrococcus luteus has a molecular weight of 34 ± 4 kDa. The kinetic parameters of bacterial lysis for all enzymes have been determined; in particular, optimal pH values for each of the substrates used have been found. For the identification of the enzymes, trypsinolysis and a mass-spectroscopic study of fragments have been carried out. The results were compared with the data on sheep proteins available in the Swiss-Prot, NCBI, and MSDB databases. 相似文献
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E. P. Delver N. G. Belogurova E. E. Tupikova S. D. Varfolomeyev A. A. Belogurov 《Molecular & general genetics : MGG》1996,253(1-2):166-172
The 1882-bp nucleotide sequence of the cryptic plasmid pNB2 isolated from the thermophilic bacterium Clostridium thermosaccharolyticum was determined. pNB2 DNA has very low GC content (27%) and may serve as a model for studying the modes of maintenance and
replication of AT-rich DNA under conditions of thermophilic growth. The plasmid sequence revealed three open reading frames
(ORFs) which would encode polypeptides of 289, 68 and 59 amino acids, respectively, and these proteins were synthesized in
E. coli extracts primed with the plasmid. We found that the product of ORF289 may be initiated at the non-ATG start codon, TTG, and
has similarities with the conserved motifs of Rep proteins encoded by rolling circle (RC) plasmids of the pC194/pUB110 family.
Southern hybridization analysis of lysates of C. thermosaccharolyticum cells harboring pNB2 revealed single-stranded intermediates, suggesting that this plasmid is able to replicate in clostridial
cells via the RC mechanism. The most significant similarities are found between pNB2 Rep protein and the Rep proteins of three
RC plasmids of the pC194 family (pTB913, pBC1 and pST1) isolated from thermophilic bacteria. Comparative analysis of these
Rep proteins showed that despite the significant level of divergence, these Rep proteins share a high degree of similarity
in the regions of five well-known conserved domains of RC Rep proteins and fall into two groups in accordance with the similarities
found in their active sites.
Received: 25 April 1996 / Accepted: 20 June 1996 相似文献
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E. P. Delver N. G. Belogurova E. E. Tupikova S. D. Varfolomeyev A. A. Belogurov 《Molecular genetics and genomics : MGG》1996,253(1-2):166-172
The 1882-bp nucleotide sequence of the cryptic plasmid pNB2 isolated from the thermophilic bacterium Clostridium thermosaccharolyticum was determined. pNB2 DNA has very low GC content (27%) and may serve as a model for studying the modes of maintenance and replication of AT-rich DNA under conditions of thermophilic growth. The plasmid sequence revealed three open reading frames (ORFs) which would encode polypeptides of 289, 68 and 59 amino acids, respectively, and these proteins were synthesized in E. coli extracts primed with the plasmid. We found that the product of ORF289 may be initiated at the non-ATG start codon, TTG, and has similarities with the conserved motifs of Rep proteins encoded by rolling circle (RC) plasmids of the pC194/pUB110 family. Southern hybridization analysis of lysates of C. thermosaccharolyticum cells harboring pNB2 revealed single-stranded intermediates, suggesting that this plasmid is able to replicate in clostridial cells via the RC mechanism. The most significant similarities are found between pNB2 Rep protein and the Rep proteins of three RC plasmids of the pC194 family (pTB913, pBC1 and pST1) isolated from thermophilic bacteria. Comparative analysis of these Rep proteins showed that despite the significant level of divergence, these Rep proteins share a high degree of similarity in the regions of five well-known conserved domains of RC Rep proteins and fall into two groups in accordance with the similarities found in their active sites. 相似文献
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Del'ver EP Sobennikova MV Belogurova NG Nekrasov SV Kolesnikova MD Agafonova OV Belogurov AA 《Molekuliarnaia biologiia》2002,36(6):1062-1067
A study was made of the functional role of the ArdA antirestriction motif (130-LLADVPETVALYFD-143) conserved among all known Ard (alleviation of restriction of DNA) proteins, which are encoded by self-transmissible plasmids and specifically inhibit type I restriction-modification systems. Conserved residues of the motif were individually changed, and the resulting mutants tested for in vivo activity. Hydrophobic L130, L131, and V138 were substituted with negatively charged E; negatively charged D133, E136, and D143 substituted with hydrophobic V; and D127, D150, and D154 neighboring the antirestriction motif substituted with V. Four substitutions (L130E, L131E, V138E, and D143V) substantially (25-1000 times) reduced the ArdA activity. The other substitutions within or beyond the motif had no appreciable effect. Substitutions L130A and L131A each reduced the ArdA activity 10- to 20-fold, indicating that high hydrophobicity of L130 and L131 is important for the ArdA function. Thus, the antirestriction role of ArdA is indeed due to its conserved motif. 相似文献
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P. A. Levashov S. A. Sedov N. G. Belogurova S. V. Shipovskov A. V. Levashov 《Biochemistry. Biokhimii?a》2012,77(11):1312-1314
In this paper we report the discovery of bacteriolytic activity of an immune system cytokine mediator, interleukin-2. Bacteriolytic activity of interleukin-2 was compared with a well-known bacteriolytic enzyme — chicken egg white lysozyme — by monitoring the lysis of the Gram-negative bacterium Escherichia coli, the Gram-positive coccus Micrococcus luteus, and the Gram-positive spore-forming bacillus Bacillus subtilis. It was found that interleukin-2 has greater specificity to the Gram-negative bacterium E. coli than does lysozyme. In contrast to chicken egg white lysozyme, interleukin-2 does not lyse the Gram-positive coccus M. luteus and the Gram-positive spore-forming bacillus B. subtilis. These results give a new understanding of the biological functions of interleukin-2, a regulatory protein that plays a role in oncological and infectious diseases. 相似文献