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1.
The oxidants of the SH groups (o-iodozobenzoate, oxidized glutathione, etc.) and the divalent cations of some metals (Zn2+ and Cd2+) significantly slow down the rate of inactivation by the protein inhibitor of the isolated F1-ATPase and ATPase in submitochondrial particles. Modification of SH groups in the ATPase does not change the rate of inactivation but completely prevents the effect of oxidants. 相似文献
2.
W. Kelley Thomas J. T. Vida Linda M. Frisse Manuel Mundo James G. Baldwin 《Journal of nematology》1997,29(3):250-254
To effectively integrate DNA sequence analysis and classical nematode taxonomy, we must be able to obtain DNA sequences from formalin-fixed specimens. Microdissected sections of nematodes were removed from specimens fixed in formalin, using standard protocols and without destroying morphological features. The fixed sections provided sufficient template for multiple polymerase chain reaction-based DNA sequence analyses. 相似文献
3.
Paola A Gilardoni Stefan Schuck Ruth Jüngling Björn Rotter Ian T Baldwin Gustavo Bonaventure 《BMC plant biology》2010,10(1):66
Background
Plants trigger and tailor defense responses after perception of the oral secretions (OS) of attacking specialist lepidopteran larvae. Fatty acid-amino acid conjugates (FACs) in the OS of the Manduca sexta larvae are necessary and sufficient to elicit the herbivory-specific responses in Nicotiana attenuata, an annual wild tobacco species. How FACs are perceived and activate signal transduction mechanisms is unknown. 相似文献4.
5.
6.
Cell-wall lysing enzymes and products of cell-wall digestion elicit ethylene in citrus 总被引:2,自引:0,他引:2
Ethylene production was induced in Valencia oranges [ Citrus sinensis (L.) Osbcck] by injection of the fungal enzyme mixture Pectolyase ( Aspergillus japonicus ) which contains pectolytic enzymes into the peel. The mixture also stimulated production of 1-aminocyclopropane-1-carboxylic acid (ACC). Cycloheximide partially inhibited the Pectolyase-induced ethylene response. Pectin fragments, resulting from partial acid hydrolysis or Pectolyase digestion, caused an increase in ethylene production when injected into the peel of intact orange fruits. Pectic fragments produced by fungal enzymes are known to be elicitors of phytoalexins and in this study are shown to elicit ethylene in citurs. 相似文献
7.
The three-dimensional structure of the deoxycholate-treated form of purple membrane has been determined to a resolution of about 6 Å. Using low temperature electron diffraction data, room temperature electron microscope images and improved methods of data analysis, higher resolution has been reached than was obtained using native membranes of the same size. Statistical analysis of the data shows that the new map is considerably better than earlier maps. The map indicates the probable sites for the lipid molecules that remain in the deoxycholate-treated membranes; some of these sites differ from those suggested by the projection map of Glaeser et al. (1985). Comparison of the bacteriorhodopsin structures now determined independently from three crystal forms shows that the monomer structure is independent of the detailed contacts with lipid molecules. The average of the three structures gives a picture with very little noise showing seven similar rod-like features which are clearly best interpreted as -helices; there is no indication that part of the structure is -sheet as suggested by Jap et al. (1983). Phases from the averaged structure at 6 Å resolution will enable better refinement of the parameters that will be required in the analysis of higher resolution images from tilted specimens needed to extend the projection map at 3.5 Å resolution (Henderson et al. 1986) to produce a three-dimensional atomic resolution map. 相似文献
8.
Summary The root systems ofEucalyptus grandis W. Hill ex Maiden, irrigated with recycled municipal effluent at two sites in north-western Victoria, Australia, were studied
by excavation and coring. Trees at Robinvale were four years-old and were irrigated using micro-sprays that covered only 70%
of the ground surface area, whereas at Mildura, effuent was uniformly was uniformly applied to six years-old trees by flood
and sprinkler irrigation.
At Mildura where roots were excavated from a 2.80×2.80×1.20 m block of soil, a total root length of 1193 m.m−2 and a total root weight of 3.1 kg m−2 were estimated in the top metre. For roots >1 mm diameter, 77% of intercepts were at 0–30 cm, whereas only 50% were in the
50–100 cm soil horizon.
At both sites where roots in the top 30 cm were studied by coring, the vertical distributions of root intercepts, length and
weight were similar. Root length was greatest in the 0–10 cm soil horizon at both sites, and intercepts of roots <1 mm diameter
comprised 73% and 81% of all roots at Mildura and Robinvale respectively. Roots <1 mm diameter contributed 85% of total length
at both sites, but only 19% and 21% of total weight at Mildura and Robinvale respectively. The horizontal distribution of
roots differed at the two sites. With uniform application of effuent at Mildura, root intercepts and length were concentrated
in the centre of the irrigation bay, but at Robinvale, the concentration occurred closer to the tree row due mainly to the
different method of irrigation. Root weight at both sites was highest within 50 cm of the tree row.
Root densities of 0.11 to 0.57 cm cm−3 were estimated in the two plantations; these were similar to root densities measured inPinus radiata D. Don plantations up to 46 months old, but were considerably lower than those estimated for pastures. The implications of
the results for the management of irrigated plantations of eucalypts are discussed. 相似文献
9.
Action mechanism of Escherichia coli DNA photolyase. II. Role of the chromophores in catalysis 总被引:3,自引:0,他引:3
DNA photolyase repairs pyrimidine dimers in DNA in a reaction that requires visible light. Photolyase from Escherichia coli is normally isolated as a blue protein and contains 2 chromophores: a blue FAD radical plus a second chromophore that exhibits an absorption maximum at 360 nm when free in solution. Oxidation of the FAD radical is accompanied by a reversible loss of activity which is proportional to the fraction of the enzyme flavin converted to FADox. Quantitative reduction of the radical to fully reduced FAD causes a 3-fold increase in activity. The results show that a reduced flavin is required for activity and suggest that flavin may act as an electron donor in catalysis. Comparison of the absorption spectrum calculated for the protein-bound second chromophore (lambda max = 390 nm) with fluorescence data and with the relative action spectrum for dimer repair indicates that the second chromophore is the fluorophore in photolyase and that it does act as a sensitizer in catalysis. On the other hand, enzyme preparations containing diminished amounts of the second chromophore do not exhibit correspondingly lower activity. This suggests that reduced flavin may also act as a sensitizer in catalysis. The blue color of the enzyme is lost upon reduction of the FAD radical. The fully reduced E. coli enzyme exhibits absorption and fluorescence properties very similar to yeast photolyase. This indicates that the two enzymes probably contain similar chromophores but are isolated in different forms with respect to the redox state of the flavin. 相似文献
10.
Structure of cDNA clones coding for human type II procollagen. The alpha 1(II) chain is more similar to the alpha 1(I) chain than two other alpha chains of fibrillar collagens. 总被引:8,自引:0,他引:8
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C T Baldwin A M Reginato C Smith S A Jimenez D J Prockop 《The Biochemical journal》1989,262(2):521-528
Overlapping cDNA clones were isolated for human type II procollagen. Nucleotide sequencing of the clones provided over 2.5 kb of new coding sequences for the human pro alpha 1(II) gene and the first complete amino acid sequence of type II procollagen from any species. Comparison with published data for cDNA clones covering the entire lengths of the human type I and type III procollagens made it possible to compare in detail the coding sequences and primary structures of the three most abundant human fibrillar collagens. The results indicated that the marked preference in the third base codons for glycine, proline and alanine previously seen in other fibrillar collagens was maintained in type II procollagen. The domains of the pro alpha 1(II) chain are about the same size as the same domains of the pro alpha chains of type I and type III procollagens. However, the major triple-helical domain is 15 amino acid residues less than the triple-helical domain of type III procollagen. Comparison of hydropathy profiles indicated that the alpha chain domain of type II procollagen is more similar to the alpha chain domain of the pro alpha 1(I) chain than to the pro alpha 2(I) chain or the pro alpha 1(III) chain. The results therefore suggest that selective pressure in the evolution of the pro alpha 1(II) and pro alpha 1(I) genes is more similar than the selective pressure in the evolution of the pro alpha 2(I) and pro alpha 1(III) genes. 相似文献