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新疆块根芍药(Paeonia anomala)内生细菌XJU-PA-1的鉴定及特性分析 总被引:1,自引:0,他引:1
目的:从新疆块根芍药(Paeonia anomala)内生菌中筛选出1株对植物病原菌有抑菌活性的内生菌并对其进行鉴定及特性分析.方法:采用琼脂扩散法进行抑菌实验,基于形态特征,生理生化特性,16S rDNA序列分析并G+C mol%含量对XJU-PA-1进行鉴定.结果:XJU-PA-1对玉米小班病菌和苹果半点落叶病菌有抑菌活性,抑菌圈直径都超过20mm.XJU-PA-1与DQ010109 Bacillus subtilis的16S rDNA序列的同源性达到100%,G+Cmol%含量为54.5%.结论:XJU-PA-1对两种致病菌有较强的抑菌能力,被鉴定为枯草芽孢杆菌(Bacillus subtilis) ,在GenBank数据库的注册号为EU741698. 相似文献
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Xiaoning Han Michael Chen Fushun Wang Martha Windrem Su Wang Steven Shanz Qiwu Xu Nancy Ann Oberheim Lane Bekar Sarah Betstadt Alcino J. Silva Takahiro Takano Steven A. Goldman Maiken Nedergaard 《Cell Stem Cell》2013,12(3):342-353
Download : Download video (32MB) 相似文献
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Isolated ACTH deficiency is an uncommon cause of secondary adrenocortical insufficiency and accompaniment with primary empty sella has been reported in several cases. We present a case of isolated ACTH deficiency associated with empty sella. A sixty-two year old woman was admitted to our endocrine clinic with complaints of weakness, fatigue, weight loss, nausea, vomiting, and lack of appetite for about one month. Physical examination indicated orthostatic hypotension and epigastric tenderness. Laboratory investigations revealed hypoglycemia, hyponatremia and anemia, in addition low plasma cortisole and ACTH levels. Serum cortisole responses to short and prolonged ACTH stimulation were tested and partial and accurate responses were obtained, respectively. Plasma ACTH and serum cortisole levels failed to respond after intravenous injection of human corticotropin releasing hormone. Other hypophysial hormone levels were within the normal reference ranges. Although cranial and abdominal computerized tomography images were evaluated as normal, cranial magnetic resonance imaging of the pituitary gland revealed 'primary empty sella turcica'. Replacement therapy with methylprednisolon resulted in the improvement of hypoglycemia, hyponatremia and clinical symptoms. Based on these results, the patient was diagnosed as isolated ACTH deficiency and was scheduled for follow up by our outpatient clinic. Our report is consistent with other reports pointing out that primary empty sella may be responsible for pathogenesis of isolated ACTH deficiency. 相似文献
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Interleukin-10 (IL-10) gene polymorphism as a potential host susceptibility factor in tuberculosis 总被引:5,自引:0,他引:5
Oral HB Budak F Uzaslan EK Baştürk B Bekar A Akalin H Ege E Ener B Göral G 《Cytokine》2006,35(3-4):143-147
Several genes encoding for different cytokines may play crucial roles in host susceptibility to tuberculosis (TB), since the cytokine production capacity varies among individuals and depends on the cytokine gene polymorphism. The association of the cytokine gene polymorphisms with the development of TB was investigated in this study. DNA samples were obtained from a Turkish population of 81 patients with the different clinical forms of TB, and 50 healthy control subjects. All genotyping (IL-6, IL-10, IFN-gamma, TGF-beta and TNF-alpha) experiments were performed using sequence-specific primers PCR (PCR-SSP). Analysis of allele frequencies showed that IL-10 -1082 G allele frequency was significantly more common in TB patients than healthy controls (37.7% vs 23.0%, p: 0.014). No statistically significant differences were observed between the different clinical forms of the disease. These results suggest that the polymorphisms in IL-10 gene may affect susceptibility to TB and increase risk of developing the disease. To confirm the biological significance of our results, further studies should be performed on other population groups. 相似文献
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Listeria monocytogenes, although uncommon as a cause of illness in the general population, can result in serious illness when it affects pregnant women, neonates, the elderly, and immunocompromised individuals. Typically, it is a food-borne organism. This report describes a case of brain-stem listeriosis in a previously healthy 51-year-old woman. The diagnosis was based on clinical findings, the results of cerebrospinal fluid (CSF) analysis, CSF culture, and magnetic resonance imaging (MRI) findings. MRI demonstrated upper brain stem and cerebellar peduncle involvement. In addition, Tc-99m exametazime (HMPAO)-labeled single photon emission computed tomography (SPECT) of the brain revealed bilateral cerebellar hypoperfusion. Antibiotic therapy resulted in partial clinical recovery after 3 weeks. At the end of 6 months, brain-stem findings had nearly resolved. However, although minimal residual findings were observed on MRI at 6 months, bilateral diffuse cerebellar hypoperfusion remained on Tc-99m HMPAO brain SPECT. 相似文献
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Exonuclease 1 (Exo 1) is an important nuclease involved in mismatch repair system that contributes to maintain genomic stability,
to modulate DNA recombination, and to mediate cell cycle arrest. A guanine (G)/adenine (A) common single nucleotide polymorphism
at first position of codon 589 in Exo 1 gene determines a glutamic acid (Glu, E) to lysine (Lys, K) (K589E) aminoacidic substitution
which may alter cancer risk by influencing the activity of Exo 1 protein. Exo 1 K589E polymorphism has been studied in various
cancers, but its association with hepatocellular carcinoma (HCC) has yet to be investigated. To determine the association
of the Exo 1 K589E polymorphism with the risk of HCC development in a Turkish population, a hospital-based case–control study
was designed consisting of 224 subjects with HCC and 224 cancer-free control subjects matched for age, gender, smoking and
alcohol status. The genotype frequency of the Exo 1 K589E polymorphism was determined by using a polymerase chain reaction–restriction
fragment length polymorphism assay. Our data shows that the Lys/Lys genotype of the Exo 1 K589E polymorphism is associated
with increased risk of HCC development in this Turkish population [odds ratio (OR) = 2.15, 95% confidence interval (CI): 1.13–4.09,
P = 0.02]. Furthermore, according to stratified analysis, a significant association was observed between the homozygote Lys/Lys
genotype and HCC risk in the subgroups of male gender (OR = 2.67, 95% CI: 1.27–5.61, P = 0.009) and patients with non-viral-related HCC (OR = 3.14, 95% CI: 1.09–8.99, P = 0.03). Because our results suggest for the first time that the Lys/Lys homozygote genotype of Exo 1 K589E polymorphism
may be a genetic susceptibility factor for HCC in the Turkish population, further independent studies are required to validate
our findings in a larger series, as well as in patients of different ethnic origins. 相似文献
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Sümbül AT Akkız H Bayram S Bekar A Akgöllü E Sandıkçı M 《Molecular biology reports》2012,39(2):1639-1647
The tumor suppressor p53 gene plays a crucial role in preventing carcinogenesis through its ability to induce cell cycle arrest
and apoptosis following DNA damage and oncogene activation. A guanine (G)/cytosine (C) common single nucleotide polymorphism
(SNP) at second position of codon 72 in exon 4 of p53 gene determines a arginine (Arg) to proline (Pro) (Arg72Pro) aminoacidic
substitution within the proline-rich domain of p53 protein. Arg72 and Pro72 allele are different from a biochemical and biological
point of view and many reports suggest that they can modulate individual cancer susceptibility. To determine the association
of the p53 Arg72Pro polymorphism with the risk of hepatocellular carcinoma (HCC) development in a Turkish population, a hospital-based
case–control study was designed consisting of 119 subjects with HCC and 119 cancer-free control subjects matched for age,
gender, smoking and alcohol status. The genotype frequency of the p53 Arg72Pro polymorphism was determined by using a polymerase
chain reaction-restriction fragment length polymorphism (PCR-RFLP) assay. Our data shows that the Pro/Pro genotype of the
p53 Arg72Pro polymorphism is associated with increased risk of HCC development in this Turkish population (OR = 3.20, 95%
CI: 1.24–8.22, P = 0.02). Furthermore, according to stratified analysis, a significant association was observed between the homozygote Pro/Pro
genotype and HCC risk in the subgroups of male gender (OR = 3.01, 95% CI: 1.14–7.97, P = 0.03) and patients with hepatitis B virus (HBV)-related HCC (OR = 4.04, 95% CI: 1.46–11.15, P = 0.007). Because our results suggest for the first time that the Pro/Pro homozygote of p53 Arg72Pro polymorphism may be
a genetic susceptibility factor for HCC (especially in the male gender and HBV-infected patients) in the Turkish population,
further independent studies are required to validate our findings in a larger series, as well as in patients of different
ethnic origins. 相似文献
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