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S. J. O'Brien P. Joslin G. L. Smith R. Wolfe N. Schaffer E. Heath J. Ott-Joslin P. P. Rawal K. K. Bhattacharjee J. S. Martenson 《Zoo biology》1987,6(2):99-116
The Asiatic lion (Panthera leo persica) exists in the wild as a single relict population of approximately 250 individuals in the protected Gir Forest Sanctuary in western India. In 1981, a species survival plan (SSP) for the Asiatic lion was established by the American Association of Zoological Parks and Aquariums to manage the 200 + descendants of Asiatic lions in captivity in western zoological facilities. This captive population was derived from seven founders. In order to compare the genetic structure of the Gir Forest population with that of the captive SSP population, a genetic survey of 46 electrophoretic allozyme systems resolved from extracts of lion blood was undertaken by using 29 SSP Asiatic lions and 28 wild-caught or captive-bred lions maintained at the Sakkarbaug Zoo in India but originally derived from the Gir Forest. The Gir lion population was found to be genetically monomorphic at each of 46 allozyme loci. This was in contrast to several African lion (Panthera leo leo) populations, which show moderate levels of allozyme variation at the same loci. The SSP lion population was polymorphic at three allozyme loci (IDHI, TF, and PTI) for alleles that were previously found only in African lion populations. Pedigree analysis of the genetic transmission of these three biochemical loci demonstrated that two of the five primary founder animals of the SSP Asiatic lion population (a breeding pair originally imported from the Trivandrum Zoo in southern India) were descendants of the African subspecies. Three other founder animals were pure Asian. A retrospective SSP pedigree analysis of two morphologic characters (prominent abdominal fold and pairing of infraorbital foramen) that are partially diagnostic for persica vs leo was consistent with this conclusion as well. The implications for the management of small captive populations of threatened species and of the Asiatic lion SSP population are discussed. 相似文献
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MNNG-induced killing of V79 cells has been found to be enhanced on inhibition of topoisomerase II activity by nalidixic acid and poly(ADP-ribose) polymerase synthesis by benzamide. Using these 2 inhibitors in conjunction after MNNG treatment, some overlap in the functions of these 2 enzymes was observed. Nalidixic acid and benzamide were found to suppress the yields of mutations and SCEs induced by MNNG. Benzamide was more effective in suppressing the mutation yield whereas nalidixic acid was more effective in suppressing SCEs. A model based on the relative requirement of topoisomerase and poly(ADP-ribose) for the repair of different types of damage has been proposed to explain the results. 相似文献
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An in vitro procedure for large scale multiplication of Sterculia urens Roxb. (Gum Kadaya Tree) has been developed using cotyledonary node segments. An average of 4.0 shoots per node were obtained on Murashige and Skoog's (MS) medium containing 2.0 mgl–1 6-benzyl amino-purine (BAP) within 21 days of initial culture. Upon subsequent subculture 16 shoots/node could be harvested every three weeks and upto three times. Sixty per cent of the shoots were successfully rooted. Rooted plantlets were transferred to plastic pots containing soil under mist house conditions before they were finally exposed to an external environment. Fifty seven per cent of the plantlets survived in nursery sheds. 相似文献
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Protoplasma - Picrorhiza kurroa Royle ex Benth is a valuable medicinal herb of North-Western Himalayas due to presence of two major bioactive compounds, picroside-I and picroside-II used in the... 相似文献
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B. Bhattacharjee Gynheung An H. S. Gupta 《Journal of plant biochemistry and biotechnology.》1997,6(2):69-73
We report production of fertile transgenic Indica rice plants by transferring a chimaeric construct consisting of promoter, first exon and intron of maize ubiquitin gene (Ubi-1) and the coding sequences of the bar gene from Streptomyces hygroscopicus to the rice protoplasts through electroporation. In total, 11 plants were regenerated. All of them were fertile and set seeds on maturity. These plants were resistant to high concentration of PPT (400 mg l?1) which was otherwise toxic to the untransformed controls. The gene was inherited to the progenies of the five plants in Mendelian ratio. 相似文献
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Shah, Ashish R., Thomas G. Keens, and David Gozal.Effect of supplemental oxygen on supramaximal exercise performance and recovery in cystic fibrosis. J. Appl.Physiol. 83(5): 1641-1647, 1997.The effects ofsupplemental O2 on recovery fromsupramaximal exercise and subsequent performance remain unknown. Ifrecovery from exercise could be enhanced in individuals with chroniclung disease, subsequent supramaximal exercise performance could also be improved. Recovery from supramaximal exercise and subsequent supramaximal exercise performance were assessed after 10 min of breathing 100% O2 or room air(RA) in 17 cystic fibrosis (CF) patients [25 ± 10 (SD) yrold, 53% men, forced expired volume in 1 s = 62 ± 21%predicted] and 17 normal subjects (25 ± 8 yr old, 59% men,forced expired volume in 1 s = 112 ± 15% predicted). Supramaximalperformance was assessed as the work of sustained bicycling at a loadof 130% of the maximum load achieved during a graded maximal exercise.Peak minute ventilation(E) andheart rate (HR) were lower in CF patients at the end of eachsupramaximal bout than in controls. In CF patients, single-exponentialtime decay constants indicated faster recovery of HR(HR = 86 ± 8 and 73 ± 6 s in RA and O2,respectively, P < 0.01). Similarly, fast and slow time constants of two-exponential equations providing thebest fit for ventilatory recovery were improved in CF patients duringO2 breathing ( = 132.1 ± 10.5 vs. 82.5 ± 10.4 s; = 880.3 ± 300.1 vs. 368.6 ± 107.1 s,P < 0.01). However, no such improvements occurred in controls. Supramaximal performance after O2 improved in CF patients (109 ± 6% of the 1st bout after O2 vs. 94 ± 6% in RA, P < 0.01).O2 supplementation had no effect on subsequent performance in controls (97 ± 3% inO2 vs. 93 ± 3% in RA). Weconclude that supplemental O2after a short bout of supramaximal exercise accelerates recovery andpreserves subsequent supramaximal performance in patients with CF. 相似文献
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Mausumi Mutsuddi Debasish Mutsuddi A. S. Mukherjee Ashish K. Duttagupta 《Chromosoma》1984,89(1):55-62
The chromosome complement of hybrid males from the cross between Drosophila miranda female and D. persimilis male provides an interesting chromosomal situation where an autosome, the 3rd chromosome of D. persimilis, coexists with a homologue that developed into a sex chromosome, the X2 in D. miranda. Except for certain inversions and a few minor translocations, these two chromosomes (X2 and the 3rd) still look alike as polytene elements. However, in hybrid males pairing of the two chromosomes, the X2 and 3rd, is rare, while in female hybrids it occurs frequently. — 3H-TdR labeling shows that while the X2 and 3rd chromosomes replicate synchronously in hybrid female, in the hybrid male the former completes its replication earlier than the 3rd chromosome, as do the two arms of the X1 (XL and XR). The frequency and relative intensity of 3H-TdR labeling of each site of the X2 and that of the 3rd chromosome in hybrid males closely agree with those of the corresponding sites in the X2 of the miranda male and the 3rd chromosome of the persimilis male (or female), respectively. The results suggest that timing and rate of replication of the X2 are determined autonomously and follow the pattern in the respective parental species. 相似文献