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排序方式: 共有134条查询结果,搜索用时 15 毫秒
1.
Summary DNA haplotype constellations of the β-globin gene cluster have been analyzed in German families with hemoglobinopathies (Hb
Freiburg, Hb K?ln, Hb Presbyterian) and β-thalassemias. The polymorphis patterns obtained were compared to those found in
families from Greece, Italy, and Turkey affected by β-thalassemia syndromes. With the combined analysis of seven restriction
site polymorphisms a DNA-diagnostic prediction for additional offspring could be made with an overall frequency of 75% in
the four ethnic groups. 相似文献
2.
The effects of intraperitoneally or intracerebrally (DA A-10 area) administered substance P (SP) on locomotor activity of rats were studied in an exact 12-h light/12-h dark cycle changing from dark to light at 6 a.m. SP was administered either at 11 a.m. (light phase, minimal locomotor activity) or at 7 p.m. (dark phase, maximal locomotor activity). The effects of 12.5 micrograms/kg SP intracerebral and 125 micrograms/kg SP intraperitoneal were very similar. In the light phase SP produces excitation but inhibition of locomotion in darkness. Hence, the effect of SP depends on the internal mechanisms controlling motor activity and tends to level off the spontaneous circadian oscillation. We found a long lasting SP effect during both the light and dark period. The present experiments led us to the conclusion that SP has a levelling effect on locomotor activity. Probably this effect might be explained as SP's action on the dopaminergic pathway or dopamine metabolism, because the dopamine content in neurons also has a circadian rhythm. 相似文献
3.
Hans Oehme 《Journal of Ornithology》1959,100(4):363-396
Zusammenfassung Die aerodynamischen Verhältnisse des Schwingenfluges bei verschiedenen Kleinvögeln werden beschrieben. Es wird der unbeschleunigte Horizontalflug bei Mauersegler, Rauchschwalbe und rundflügeligen kleinen Sperlingsvögeln verglichen. Als Kenngrößen des Kraftfluges werden der Fortschrittsgrad, das Verhältnis Hub/Schub an der Flügelspitze (A/V) und das Gesamtauftriebs-Vortriebs-Verhältnis (Ages/V) abgeleitet. An Hand dieser Kenngrößen wird gezeigt, daß es möglich ist, einen Hubflug von einem Schubflug zu unterscheiden. Diese Unterscheidung ist weder an eine bestimmte Art der Flügelbewegung gebunden, noch charakterisiert sie als alleinige Flugweise eine bestimmte Vogelart. Die Besonderheiten des Schnellfluges und des Fluges auf der Stelle bei den untersuchten Arten werden dargestellt, wobei der Unterschied zwischen Platzrüttelflug und Platztrillerflug betont wird.Es wird gezeigt, daß die Flugeigenschaften der besprochenen Arten von ihrer Lebensweise her, besonders von der Art des Nahrungserwerbs verstanden werden können.Der Zusammenhang zwischen Flugweise und Bau des Flügels wird an einigen Beispielen erörtert. Dabei können Beziehungen zwischen den aerodynamischen Eigenschaften des Flügels und dem Bau der Federn bis hin zu deren Feinstrukturen gezeigt werden. Skelett und Muskulatur lassen zwar auch gewisse funktionell bedingte Eigenarten erkennen, doch sind eindeutige Beziehungen manchmal nicht herzustellen.Es zeigt sich, daß es möglich ist, bestimmte Struktureigenschaften aus ihrer Funktion zu begreifen, daß aber die Herleitung bestimmter Flugmodi aus Strukturmerkmalen des Flügels oft große Schwierigkeiten bereitet, und daß es hier als unmöglich angesehen werden muß, von einigen morphologischen Eigenschaften ausgehend zu richtigen Schlüssen zu kommen. 相似文献
4.
N V Komissarova W E Siems O A Gomazkov P Oehme K D Jentzsch 《Biulleten' eksperimental'no? biologii i meditsiny》1982,93(6):3-5
Substance "P" (SP) and its derivatives inhibited activity of angiotensin-converting enzyme (ACE) in human blood serum, I50 for SP was 31 microM. The same results were obtained for N-terminal fragments of the SP molecule: Arg-Pro-Lys-Pro and Lys-Pro. The C-terminal heptapeptide and the dipeptide Arg-Pro negligibly inhibited ACE activity. A possible significance of the interaction between SP and ACE in the regulation of the microhemodynamics is discussed. 相似文献
5.
Under the condition of receptor blockade produced by continuous presence of an agonist in the organ bath, it was attempted to determine the dissociation constants for C-terminal partial sequences of the substance P at an isolated guinea pig ileum, by analogy with the method involving irreversible antagonists, and to compare them with the biological activity at the guinea pig ileum and the rat colon (ED50 values). Differentiation of the biological activity at the guinea pig ileum into affinity and "intrinsic efficacy" allows one to explain quantitative differences in determining the biological value on both isolated organs, and to reveal the contribution of the individual amino acids to affinity and "intrinsic efficacy". 相似文献
6.
Adem Yildirim Sina Mozaffari-Jovin Ann-Kathrin Wallisch Jessica Schfer Sebastian E J Ludwig Henning Urlaub Reinhard Lührmann Uwe Wolfrum 《Nucleic acids research》2021,49(10):5845
Splicing is catalyzed by the spliceosome, a compositionally dynamic complex assembled stepwise on pre-mRNA. We reveal links between splicing machinery components and the intrinsically disordered ciliopathy protein SANS. Pathogenic mutations in SANS/USH1G lead to Usher syndrome—the most common cause of deaf-blindness. Previously, SANS was shown to function only in the cytosol and primary cilia. Here, we have uncovered molecular links between SANS and pre-mRNA splicing catalyzed by the spliceosome in the nucleus. We show that SANS is found in Cajal bodies and nuclear speckles, where it interacts with components of spliceosomal sub-complexes such as SF3B1 and the large splicing cofactor SON but also with PRPFs and snRNAs related to the tri-snRNP complex. SANS is required for the transfer of tri-snRNPs between Cajal bodies and nuclear speckles for spliceosome assembly and may also participate in snRNP recycling back to Cajal bodies. SANS depletion alters the kinetics of spliceosome assembly, leading to accumulation of complex A. SANS deficiency and USH1G pathogenic mutations affects splicing of genes related to cell proliferation and human Usher syndrome. Thus, we provide the first evidence that splicing dysregulation may participate in the pathophysiology of Usher syndrome. 相似文献
7.
8.
It is well-kown that sensory information influences the way we execute motor responses. However, less is known about if and how sensory and motor information are integrated in the subsequent process of response evaluation. We used a modified Simon Task to investigate how these streams of information are integrated in response evaluation processes, applying an in-depth neurophysiological analysis of event-related potentials (ERPs), time-frequency decomposition and sLORETA. The results show that response evaluation processes are differentially modulated by afferent proprioceptive information and efference copies. While the influence of proprioceptive information is mediated via oscillations in different frequency bands, efference copy based information about the motor execution is specifically mediated via oscillations in the theta frequency band. Stages of visual perception and attention were not modulated by the interaction of proprioception and motor efference copies. Brain areas modulated by the interactive effects of proprioceptive and efference copy based information included the middle frontal gyrus and the supplementary motor area (SMA), suggesting that these areas integrate sensory information for the purpose of response evaluation. The results show how motor response evaluation processes are modulated by information about both the execution and the location of a response. 相似文献
9.
Laura David Peter Schwan Martin Lobedann Sven-Oliver Borchert Bastian Budde Maike Temming Mike Kuerschner Francisca Maria Alberti Aguilo Kerstin Baumarth Tobias Thüte Benjamin Maiser Andreas Blank Viktorija Kistler Nils Weber Heiko Brandt Martin Poggel Klaus Kaiser Karl Geisen Felix Oehme Gerhard Schembecker 《Biotechnology and bioengineering》2020,117(4):1024-1036
Continuous processing is the future production method for monoclonal antibodies (mAbs). A fully continuous, fully automated downstream process based on disposable equipment was developed and implemented inside the MoBiDiK pilot plant. However, a study evaluating the comparability between batch and continuous processing based on product quality attributes was not conducted before. The work presented fills this gap comparing both process modes experimentally by purifying the same harvest material (side-by-side comparability). Samples were drawn at different time points and positions in the process for batch and continuous mode. Product quality attributes, product-related impurities, as well as process-related impurities were determined. The resulting polished material was processed to drug substance and further evaluated regarding storage stability and degradation behavior. The in-process control data from the continuous process showed the high degree of accuracy in providing relevant process parameters such as pH, conductivity, and protein concentration during the entire process duration. Minor differences between batch and continuous samples are expected as different processing conditions are unavoidable due to the different nature of batch and continuous processing. All tests revealed no significant differences in the intermediates and comparability in the drug substance between the samples of both process modes. The stability study of the final product also showed no differences in the stability profile during storage and forced degradation. Finally, online data analysis is presented as a powerful tool for online-monitoring of chromatography columns during continuous processing. 相似文献
10.
Marco Lodrini Ina Oehme Christina Schroeder Till Milde Marie C. Schier Annette Kopp-Schneider Johannes H. Schulte Matthias Fischer Katleen De Preter Filip Pattyn Mirco Castoldi Martina U. Muckenthaler Andreas E. Kulozik Frank Westermann Olaf Witt Hedwig E. Deubzer 《Nucleic acids research》2013,41(12):6018-6033