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Nande R Di Benedetto A Aimola P De Carlo F Carper M Claudio CD Denvir J Valluri J Duncan GC Claudio PP 《PloS one》2012,7(5):e37743
Fibrosarcoma is a deadly disease in cats and is significantly more often located at classical vaccine injections sites. More rare forms of spontaneous non-vaccination site (NSV) fibrosarcomas have been described and have been found associated to genetic alterations. Purpose of this study was to compare the efficacy of adenoviral gene transfer in NVS fibrosarcoma. We isolated and characterized a NVS fibrosarcoma cell line (Cocca-6A) from a spontaneous fibrosarcoma that occurred in a domestic calico cat. The feline cells were karyotyped and their chromosome number was counted using a Giemsa staining. Adenoviral gene transfer was verified by western blot analysis. Flow cytometry assay and Annexin-V were used to study cell-cycle changes and cell death of transduced cells. Cocca-6A fibrosarcoma cells were morphologically and cytogenetically characterized. Giemsa block staining of metaphase spreads of the Cocca-6A cells showed deletion of one of the E1 chromosomes, where feline p53 maps. Semi-quantitative PCR demonstrated reduction of p53 genomic DNA in the Cocca-6A cells. Adenoviral gene transfer determined a remarkable effect on the viability and growth of the Cocca-6A cells following single transduction with adenoviruses carrying Mda-7/IL-24 or IFN-γ or various combination of RB/p105, Ras-DN, IFN-γ, and Mda-7 gene transfer. Therapy for feline fibrosarcomas is often insufficient for long lasting tumor eradication. More gene transfer studies should be conducted in order to understand if these viral vectors could be applicable regardless the origin (spontaneous vs. vaccine induced) of feline fibrosarcomas. 相似文献
2.
Torres CE Gibello A Nande M Martin M Blanco A 《Applied microbiology and biotechnology》2008,78(5):889-897
Slime formation is a serious problem nowadays in the paper industry. Some enterobacteria are associated with the formation
of slime deposits in paper and board mills. Detection and characterization of slime forming bacteria, belonging to the genus
Enterobacter, Raoultella, and Klebsiella have been achieved by fluorescence in situ hybridization (FISH), using one probe based on the enterobacterial repetitive intergenic consensus sequence and other two
rRNA targeted oligonucleotide probes. The effects of three kinds of antimicrobiological products (biocides, dispersants, and
enzymes) on these enterobacterial cells were analyzed by flow cytometry (FC). Biocides Butrol 1009 and 1072 were the most effective microbiocides against all enterobacterial cells analyzed, reaching 90% of dead
bacteria after 24 h. However, the enzymatic treatment (Buzyme) was not equally efficient on enterobacteria and its microbiocide
capacity varied depending on the type of microorganism. FISH and FC were effective tools to detect important slime forming
enterobacteria and to select specific treatments to control microbial problems in the paper industry. 相似文献
3.
Sarah E. Mathis Anthony Alberico Rounak Nande Walter Neto Logan Lawrence Danielle R. McCallister James Denvir Gerrit A. Kimmey Mark Mogul Gerard Oakley Krista L. Denning Thomas Dougherty Jagan V. Valluri Pier Paolo Claudio 《PloS one》2014,9(8)
Administration of ineffective anticancer therapy is associated with unnecessary toxicity and development of resistant clones. Cancer stem-like cells (CSLCs) resist chemotherapy, thereby causing relapse of the disease. Thus, development of a test that identifies the most effective chemotherapy management offers great promise for individualized anticancer treatments. We have developed an ex vivo chemotherapy sensitivity assay (ChemoID), which measures the sensitivity of CSLCs as well as the bulk of tumor cells to a variety of chemotherapy agents. Two patients, a 21-year old male (patient 1) and a 5-month female (patient 2), affected by anaplastic WHO grade-III ependymoma were screened using the ChemoID assay. Patient 1 was found sensitive to the combination of irinotecan and bevacizumab, which resulted in a prolonged disease progression free period of 18 months. Following recurrence, the combination of various chemotherapy drugs was tested again with the ChemoID assay. We found that benzyl isothiocyanate (BITC) greatly increased the chemosensitivity of the ependymoma cells to the combination of irinotecan and bevacizumab. After patient 1 was treated for two months with irinotecan, bevacizumab and supplements of cruciferous vegetable extracts containing BITC, we observed over 50% tumoral regression in comparison with pre-ChemoID scan as evidenced by MRI. Patient 2 was found resistant to all treatments tested and following 6 cycles of vincristine, carboplatin, cyclophosphamide, etoposide, and cisplatin in various combinations, the tumor of this patient rapidly progressed and proton beam therapy was recommended. As expected animal studies conducted with patient derived xenografts treated with ChemoID screened drugs recapitulated the clinical observation. This assay demonstrates that patients with the same histological stage and grade of cancer may vary considerably in their clinical response, suggesting that ChemoID testing which measures the sensitivity of CSLCs as well as the bulk of tumor cells to a variety of chemotherapy agents could lead to more effective and personalized anticancer treatments in the future. 相似文献
4.
The aim of this study was to investigate PEGylated rosin derivatives (PRDs) as microencapsulating materials for sustained
drug delivery. PRDs (D1, D2, and D3) composed of a constant weight of rosin and varied amounts of polyethylene glycol (PEG)
400 and maleic anhydride were synthesized in the laboratory. Microparticles were prepared by the O/O solvent evaporation technique
using the acetone/paraffin system. Diclofenac sodium (DFS) and diltiazem hydrochloride (DLTZ) were used as model drugs. The
effect of the type of PRD, drug, PRD:drug ratio, viscosity of external phase, stirring speed, concentration of magnesium stearate
(droplet stabilizer), and method of preparation on particle size, drug loading, and drug release profiles of microparticles
was investigated. PRDs could produce discrete and spherical microspheres (with DFS) and microcapsules (with DLTZ). The drug
loading value for microparticles was found to be in the range of 37.21% to 87.90%. The microparticle size range was 14 to
36 μm. The particle size and drug loadings of microparticles were substantially affected by the concentration of magnesium
stearate and the type of drug, respectively. Most of the formulations could sustain the DFS and DLTZ release for 20 hours.
DFS and DLTZ release from PRD microparticles followed Hixson-Crowell and first-order kinetics, respectively. The results suggest
that PRDs can be used successfully to prepare discrete and spherical microparticles with DFS and DLTZ for sustained drug delivery.
Published: June 22, 2007 相似文献
5.
Anjalika Nande Ben Adlam Justin Sheen Michael Z. Levy Alison L. Hill 《PLoS computational biology》2021,17(2)
In the absence of pharmaceutical interventions, social distancing is being used worldwide to curb the spread of COVID-19. The impact of these measures has been inconsistent, with some regions rapidly nearing disease elimination and others seeing delayed peaks or nearly flat epidemic curves. Here we build a stochastic epidemic model to examine the effects of COVID-19 clinical progression and transmission network structure on the outcomes of social distancing interventions. Our simulations show that long delays between the adoption of control measures and observed declines in cases, hospitalizations, and deaths occur in many scenarios. We find that the strength of within-household transmission is a critical determinant of success, governing the timing and size of the epidemic peak, the rate of decline, individual risks of infection, and the success of partial relaxation measures. The structure of residual external connections, driven by workforce participation and essential businesses, interacts to determine outcomes. We suggest limited conditions under which the formation of household “bubbles” can be safe. These findings can improve future predictions of the timescale and efficacy of interventions needed to control second waves of COVID-19 as well as other similar outbreaks, and highlight the need for better quantification and control of household transmission. 相似文献
6.
The purpose of the present study was to investigate the potential use of two PEGylated derivatives of rosin (PD) as sustained
release film forming materials. The derivatives differed chemically by their acid numbers—PD-1 with 120.93 and PD-2 with 88.19.
The derivative films were characterized for surface morphology, water uptake-weight loss, angle of contact, water vapor transmission
rate, mechanical properties and permeability study. Dissolution of diclofenac sodium (DS) and propranolol hydrochloride (PHL)
as model drugs was studied from coated pellets. The films of derivatives with and without plasticizers were smooth and continuous.
PD-2 films developed greater numbers of pores when in contact with phosphate buffer pH 6.8. The low weight loss, low angles
of contact and high water vapor transmission rate of PD-2 films were related to presence of higher concentration of PEG esters.
Higher tensile strength and percent elongation of PD-2 films was due to greater degree of internal plasticization of the derivative.
The permeability of films to model drugs propranolol hydrochloride and diclofenac sodium was inversely proportional to the
film thickness and dibutyl phthalate concentration in them; the permeability being greatest in PD-2 films containing 10% PEG
200. Dissolution rate of propranolol hydrochloride was higher from the coated pellets. The dissolution data followed zero
order, Baker–Lonsdale equation and Hixon–Crowell equation of release kinetics with high correlation coefficients. The mechanism
of drug release from these coated systems however followed class II transport (n > 1.0). The derivatives investigated could successfully retard release of the model drugs and offers an alternative to the
conventionally used polymers. 相似文献
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